UL52 (NC_001798) Virus Tagged ORF Clone

CAT#: VC100918

Myc-DDK-tagged ORF clone of viral ORF for helicase-primase primase subunit [Human herpesvirus 2], codon optimized for human cell expression, NP_044523

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UL52
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100918 represents NCBI reference of NP_044523 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGACAGAGGATTGTGACCACGAAGGACGCAGCGTCGCAGCCCCCGTCGAAGTCACAGCCCTGTATG
  CGACCGATGGCTGTGTTATCACTAGCAGCCTGGCACTGCTGACAAATTGTTTGCTTGGGGCAGAACCACT
  GTATATCTTCTCATATGACGCATATCGCAGCGATGCCCCCAACGGTCCTACAGGGGCTCCAACCGAGCAG
  GAAAGGTTCGAGGGGTCTCGGGCTCTCTATAGGGATGCCGGTGGTCTGAACGGTGATTCTTTCAGAGTGA
  CATTCTGCCTCCTGGGAACCGAAGTAGGCGTCACCCACCACCCAAAGGGGCGGACAAGACCCATGTTCGT
  CTGCAGATTTGAGAGGGCAGATGACGTCGCTGTGCTGCAGGATGCGTTGGGCAGGGGGACCCCCCTGCTG
  CCAGCTCATGTGACTGCAACCCTCGATCTGGAGGCAACCTTTGCTCTGCACGCAAATATTATAATGGCAC
  TGACAGTGGCTATTGTGCACAACGCTCCTGCACGCATTGGTTCTGGCTCCACAGCTCCTTTGTACGAACC
  TGGAGAGAGTATGCGATCTGTGGTCGGACGAATGAGCCTTGGACAGCGGGGACTGACCACACTCTTCGTG
  CACCACGAAGCCCGGGTCCTCGGCGCATACCGCCGGGCATATTATGGAAGTGCACAGAGCCCGTTTTGGT
  TTCTGTCCAAGTTTGGCCCGGATGAGAAGAGCCTCGTTCTCGCTGCACGATACTATCTGTTGCAGGCGCC
  TCGACTCGGCGGCGCAGGGGCCACCTATGACTTGCAGGCTGTCAAGGATATCTGTGCCACTTATGCTATA
  CCCCACGATCCCCGACCTGACACTCTCTCAGCAGCTAGTCTGACGAGTTTTGCTGCGATTACCAGGTTCT
  GCTGCACCTCCCAGTATTCAAGAGGGGCTGCCGCGGCAGGCTTCCCCTTGTATGTCGAAAGAAGGATCGC
  CGCTGACGTTAGGGAGACTGGTGCCCTCGAGAAGTTCATCGCGCATGACAGAAGCTGCTTGCGAGTGAGT
  GATAGGGAGTTTATTACTTATATATACTTGGCACATTTTGAGTGCTTTAGTCCCCCAAGACTGGCAACCC
  ACCTGCGGGCGGTGACCACACATGATCCTAGTCCTGCTGCAAGTACAGAGCAGCCATCCCCCTTGGGACG
  GGAGGCCGTTGAGCAGTTTTTTCGGCATGTACGAGCCCAGCTCAACATTCGCGAGTATGTTAAGCAAAAT
  GTGACACCTCGCGAAACGGCACTGGCCGGAGACGCCGCCGCTGCCTACCTGAGGGCCAGAACATATGCTC
  CAGCCGCTCTGACCCCTGCACCTGCCTATTGTGGGGTGGCCGATTCCTCTACAAAAATGATGGGGAGGCT
  GGCTGAAGCCGAGAGACTTCTGGTGCCTCATGGGTGGCCTGCATTCGCTCCTACTACCCCAGGTGATGAC
  GCCGGTGGCGGGACAGCTGCACCTCAGACATGCGGGATCGTTAAAAGGCTCCTGAAGTTGGCAGCCACAG
  AGCAACAGGGTACTACCCCCCCAGCTATTGCAGCGCTGATGCAGGACGCATCAGTGCAAACACCTCTGCC
  AGTATACAGAATTACCATGAGTCCAACTGGGCAGGCTTTCGCTGCCGCAGCCAGAGACGACTGGGCTAGG
  GTGACAAGAGATGCAAGACCCCCGGAAGCCACGGTCGTCGCAGATGCTGCTGCCGCCCCAGAGCCAGGAG
  CACTGGGAAGACGCTTGACGCGGAGAATTTGTGCACGGGGACCTGCCCTCCCTCCAGGGGGTCTTGCCGT
  AGGAGGCCAGATGTACGTTAACAGAAATGAGATTTTCAATGCCGCGCTGGCAGTAACTAACATTATCCTG
  GATTTGGATATCGCACTGAAGGAGCCCGTGCCTTTTCCTCGCCTGCACGAAGCCCTCGGCCATTTTCGCA
  GAGGTGCCCTCGCCGCCGTACAACTTTTGTTCCCAGCAGCTCGGGTGGATCCTGACGCCTATCCATGTTA
  TTTCTTCAAGAGTGCGTGCAGGCCTAGAGCTCCCCCAGTTTGTGCTGGGGACGGTCCGTCCGCCGGTGGA
  GATGATGGTGATGGGGATTGGTTCCCAGATGCAGGCGGTCCTGGAGACGAGGAATGGGAAGAGGACACAG
  ACCCCATGGATACCACTCACGGCCCCCTTCCCGATGATGAAGCCGCCTATCTCGACTTGTTGCATGAGCA
  GATTCCCGCCGCCACCCCTTCCGAGCCAGACAGTGTGGTGTGCTCATGCGCCGACAAAATCGGCCTCCGA
  GTATGCTTGCCCGTGCCCGCACCCTATGTTGTTCACGGGTCCCTGACCATGCGAGGGGTTGCGCGGGTAA
  TTCAGCAGGCAGTGCTCCTGGATCGAGATTTTGTAGAAGCCGTGGGCTCCCATGTCAAAAACTTCCTCCT
  CATCGACACAGGGGTGTATGCCCACGGTCATAGCTTGAGACTGCCGTACTTCGCCAAGATCGGTCCCGAT
  GGTAGTGCATGTGGGCGACTCCTGCCCGTGTTCGTCATTCCCCCTGCCTGCGAGGATGTTCCAGCATTTG
  TTGCTGCCCATGCAGATCCCAGGAGATTTCACTTTCACGCCCCACCTATGTTTTCAGCTGCGCCCAGGGA
  GATCAGGGTGCTCCATTCACTCGGCGGGGATTATGTCTCCTTCTTTGAAAAGAAGGCCAGCCGGAACGCT
  CTGGAGCACTTTGGAAGGCGCGAGACCCTCACAGAGGTCCTGGGCAGGTACGATGTGCGGCCAGATGCCG
  GAGAAACGGTGGAAGGCTTTGCTAGCGAACTTTTGGGCAGAATTGTGGCATGTATAGAGGCCCACTTCCC
  TGAACACGCCAGAGAATACCAAGCAGTTTCCGTCAGACGCGCAGTAATCAAGGACGATTGGGTTTTGCTC
  CAGCTGATCCCCGGTCGAGGCGCCTTGAACCAGTCTCTGTCCTGTCTCAGGTTTAAACATGGTCGAGCTA
  GTAGAGCTACAGCCCGAACATTTCTGGCCTTGTCCGTAGGCACAAATAACAGGCTGTGTGCATCCTTGTG
  CCAGCAATGTTTCGCCACCAAGTGCGATAATAACAGATTGCACACGCTCTTCACAGTGGACGCCGGCACC
  CCTTGCTCTCGGTCCGCCCCCTCCTCTACCTCACGCCCCTCCTCTAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100918 representing NP_044523
  Red=Cloning sites Green=Tags
  
  MGTEDCDHEGRSVAAPVEVTALYATDGCVITSSLALLTNCLLGAEPLYIFSYDAYRSDAPNGPTGAPTEQ
  ERFEGSRALYRDAGGLNGDSFRVTFCLLGTEVGVTHHPKGRTRPMFVCRFERADDVAVLQDALGRGTPLL
  PAHVTATLDLEATFALHANIIMALTVAIVHNAPARIGSGSTAPLYEPGESMRSVVGRMSLGQRGLTTLFV
  HHEARVLGAYRRAYYGSAQSPFWFLSKFGPDEKSLVLAARYYLLQAPRLGGAGATYDLQAVKDICATYAI
  PHDPRPDTLSAASLTSFAAITRFCCTSQYSRGAAAAGFPLYVERRIAADVRETGALEKFIAHDRSCLRVS
  DREFITYIYLAHFECFSPPRLATHLRAVTTHDPSPAASTEQPSPLGREAVEQFFRHVRAQLNIREYVKQN
  VTPRETALAGDAAAAYLRARTYAPAALTPAPAYCGVADSSTKMMGRLAEAERLLVPHGWPAFAPTTPGDD
  AGGGTAAPQTCGIVKRLLKLAATEQQGTTPPAIAALMQDASVQTPLPVYRITMSPTGQAFAAAARDDWAR
  VTRDARPPEATVVADAAAAPEPGALGRRLTRRICARGPALPPGGLAVGGQMYVNRNEIFNAALAVTNIIL
  DLDIALKEPVPFPRLHEALGHFRRGALAAVQLLFPAARVDPDAYPCYFFKSACRPRAPPVCAGDGPSAGG
  DDGDGDWFPDAGGPGDEEWEEDTDPMDTTHGPLPDDEAAYLDLLHEQIPAATPSEPDSVVCSCADKIGLR
  VCLPVPAPYVVHGSLTMRGVARVIQQAVLLDRDFVEAVGSHVKNFLLIDTGVYAHGHSLRLPYFAKIGPD
  GSACGRLLPVFVIPPACEDVPAFVAAHADPRRFHFHAPPMFSAAPREIRVLHSLGGDYVSFFEKKASRNA
  LEHFGRRETLTEVLGRYDVRPDAGETVEGFASELLGRIVACIEAHFPEHAREYQAVSVRRAVIKDDWVLL
  QLIPGRGALNQSLSCLRFKHGRASRATARTFLALSVGTNNRLCASLCQQCFATKCDNNRLHTLFTVDAGT
  PCSRSAPSSTSRPSSS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001798
• ORF Size: 3198 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001798.1, NP_044523
• RefSeq ORF: 3198
• Locus ID: 1487341
• MW: 114.7 kDa