UL48 (NC_001798) Virus Tagged ORF Clone

CAT#: VC100913

Myc-DDK-tagged ORF clone of viral ORF for transactivating tegument protein VP16 [Human herpesvirus 2], codon optimized for human cell expression, NP_044518

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UL48
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100913 represents NCBI reference of NP_044518 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCTGCTCGTCGACGATTTGTTTGCCGATGCGGACGGAGTATCTCCTCCCCCACCACGCCCGGCAG
  GCGGCCCAAAGAATACCCCAGCCGCCCCTCCCCTGTATGCGACTGGCCGGCTGTCACAGGCTCAGCTTAT
  GCCCAGCCCACCTATGCCCGTCCCACCCGCCGCCCTGTTCAATAGGCTCCTCGATGACCTCGGGTTCTCT
  GCCGGCCCTGCCCTGTGCACGATGCTGGATACATGGAACGAAGATCTTTTTTCCGGATTCCCAACAAACG
  CTGACATGTACAGGGAGTGCAAATTTCTGAGCACCCTCCCCTCTGACGTGATAGACTGGGGAGACGCTCA
  TGTCCCCGAGAGGTCCCCTATTGATATCAGAGCTCATGGCGATGTCGCTTTCCCAACGCTGCCTGCTACA
  AGAGATGAACTGCCTAGCTACTATGAGGCCATGGCTCAGTTTTTCAGGGGTGAACTGCGCGCTCGAGAGG
  AGTCCTACAGAACCGTCCTGGCCAATTTCTGCAGCGCCCTTTATCGCTACCTTCGCGCCAGCGTGAGGCA
  GCTTCATAGGCAGGCGCATATGCGGGGCAGGAACAGGGATCTGCGGGAAATGCTGAGAACGACAATCGCC
  GACAGATATTACAGGGAAACAGCAAGGTTGGCCAGGGTGTTGTTCCTGCACCTCTACCTGTTTCTGTCTC
  GAGAAATCCTGTGGGCCGCCTATGCTGAACAGATGATGCGGCCTGATCTGTTCGACGGGCTTTGCTGTGA
  TCTGGAATCCTGGAGGCAGCTGGCTTGCCTCTTCCAGCCCCTGATGTTTATAAACGGCTCCCTGACCGTT
  AGGGGGGTCCCGGTGGAGGCCAGACGACTGAGGGAACTGAATCATATTAGAGAGCATCTCAACCTGCCCC
  TGGTGCGGAGCGCAGCCGCAGAAGAACCTGGTGCTCCACTTACAACTCCCCCCGTCCTGCAAGGAAACCA
  AGCTCGCAGCTCAGGATACTTTATGCTCCTGATTAGAGCAAAACTGGACTCATATTCTAGTGTGGCCACA
  AGTGAGGGAGAGAGCGTTATGCGGGAGCACGCATACAGCCGGGGTAGAACAAGGAACAATTATGGGAGCA
  CTATTGAGGGACTCCTCGATCTTCCGGACGACGACGATGCACCCGCAGAAGCCGGACTGGTGGCCCCTCG
  GATGTCCTTTCTCTCTGCTGGTCAGCGACCCAGGCGACTGTCCACCACCGCTCCCATCACTGACGTGTCA
  TTGGGCGATGAGCTCCGGCTTGACGGTGAAGAGGTAGACATGACACCTGCCGATGCACTCGACGACTTTG
  ACCTGGAAATGCTTGGCGACGTGGAATCCCCAAGTCCCGGGATGACTCATGACCCCGTAAGTTACGGAGC
  CCTGGATGTTGATGATTTCGAATTCGAGCAAATGTTCACTGACGCTATGGGCATCGATGACTTCGGAGGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100913 representing NP_044518
  Red=Cloning sites Green=Tags
  
  MDLLVDDLFADADGVSPPPPRPAGGPKNTPAAPPLYATGRLSQAQLMPSPPMPVPPAALFNRLLDDLGFS
  AGPALCTMLDTWNEDLFSGFPTNADMYRECKFLSTLPSDVIDWGDAHVPERSPIDIRAHGDVAFPTLPAT
  RDELPSYYEAMAQFFRGELRAREESYRTVLANFCSALYRYLRASVRQLHRQAHMRGRNRDLREMLRTTIA
  DRYYRETARLARVLFLHLYLFLSREILWAAYAEQMMRPDLFDGLCCDLESWRQLACLFQPLMFINGSLTV
  RGVPVEARRLRELNHIREHLNLPLVRSAAAEEPGAPLTTPPVLQGNQARSSGYFMLLIRAKLDSYSSVAT
  SEGESVMREHAYSRGRTRNNYGSTIEGLLDLPDDDDAPAEAGLVAPRMSFLSAGQRPRRLSTTAPITDVS
  LGDELRLDGEEVDMTPADALDDFDLEMLGDVESPSPGMTHDPVSYGALDVDDFEFEQMFTDAMGIDDFGG
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001798
• ORF Size: 1470 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001798.1, NP_044518
• RefSeq ORF: 1470
• Locus ID: 1487335
• MW: 54.6 kDa