UL42 (NC_001798) Virus Tagged ORF Clone

CAT#: VC100907

Myc-DDK-tagged ORF clone of viral ORF for DNA polymerase processivity subunit [Human herpesvirus 2], codon optimized for human cell expression, NP_044512

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UL42
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100907 represents NCBI reference of NP_044512 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCACTTGCCCGGCGGGGCCGCTGCAGCCCCACTGTCTGAAGACGCGATCCCTAGCCCCCGCGAAC
  GCACCGAGGACTGGCCTCCTTGTCAGATCGTTCTGCAGGGCGCTGAGCTTAATGGAATACTGCAAGCCTT
  CGCACCCCTCAGGACTAGCCTCCTGGATAGTTTGCTTGTGGTTGGCGATAGGGGCATCCTGGTTCACAAC
  GCGATCTTCGGAGAACAGGTCTTTTTGCCACTGGATCATTCTCAATTTTCACGCTACAGGTGGGGAGGTC
  CAACAGCTGCTTTCTTGAGTCTCGTAGATCAGAAGCGGAGCCTGTTGTCCGTGTTTCGGGCCAATCAGTA
  TCCCGACCTGAGGCGCGTTGAACTGACCGTGACAGGGCAGGCACCCTTCAGAACATTGGTCCAGCGCATC
  TGGACTACAGCAAGTGATGGAGAGGCCGTGGAGCTTGCCTCCGAAACTCTGATGAAACGGGAACTCACTA
  GCTTTGCTGTTTTGCTTCCCCAGGGGGACCCAGACGTGCAACTGAGACTCACAAAACCTCAGCTGACCAA
  GGTCGTCAACGCGGTGGGCGATGAGACTGCCAAGCCCACTACGTTTGAGCTCGGCCCCAATGGAAAGTTC
  AGCGTTTTTAATGCTCGCACATGCGTTACTTTCGCAGCTCGGGAAGAAGGGGCCAGTAGCTCAACATCTG
  CGCAGGTCCAGATCCTCACCTCAGCCTTGAAGAAGGCTGGCCAGGCGGCCGCCAACGCCAAAACCGTGTA
  TGGCGAAAACACCCACCGAACCTTCTCCGTGGTTGTGGACGATTGTTCTATGAGAGCGGTTCTGCGGCGG
  CTGCAGGTGGGAGGCGGTACCCTCAAGTTCTTCCTCACCGCCGATGTGCCATCCGTCTGCGTCACTGCCA
  CAGGACCGAATGCGGTTTCCGCCGTGTTCCTGCTCAAGCCTCAACGAGTGTGCCTGAATTGGCTCGGGAG
  GTCTCCCGGTTCTAGCACAGGATCACTTGCCTCACAGGACTCTCGCGCAGGCCCTACCGATAGCCAAGAT
  AGCTCAAGTGAGCCCGACGCCGGCGATCGGGGCGCTCCAGAAGAGGAAGGCCTTGAAGGTCAAGCTCGGG
  TACCCCCGGCCTTTCCAGAACCGCCCGGCACAAAGCGAAGACATCCAGGCGCCGAGGTTGTACCCGCCGA
  TGACGCCACCAAACGACCCAAAACCGGAGTGCCCGCAGCTCCGACTCGGGCTGAGAGTCCTCCCTTGAGT
  GCCAGGTACGGACCCGAAGCCGCTGAGGGAGGCGGCGATGGGGGACGATATGCATGTTATTTCAGAGACC
  TCCAGACAGGAGATGCCAGCCCTTCTCCTCTGAGTGCCTTCAGAGGCCCACAACGGCCGCCCTACGGTTT
  TGGCCTGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100907 representing NP_044512
  Red=Cloning sites Green=Tags
  
  MAHLPGGAAAAPLSEDAIPSPRERTEDWPPCQIVLQGAELNGILQAFAPLRTSLLDSLLVVGDRGILVHN
  AIFGEQVFLPLDHSQFSRYRWGGPTAAFLSLVDQKRSLLSVFRANQYPDLRRVELTVTGQAPFRTLVQRI
  WTTASDGEAVELASETLMKRELTSFAVLLPQGDPDVQLRLTKPQLTKVVNAVGDETAKPTTFELGPNGKF
  SVFNARTCVTFAAREEGASSSTSAQVQILTSALKKAGQAAANAKTVYGENTHRTFSVVVDDCSMRAVLRR
  LQVGGGTLKFFLTADVPSVCVTATGPNAVSAVFLLKPQRVCLNWLGRSPGSSTGSLASQDSRAGPTDSQD
  SSSEPDAGDRGAPEEEGLEGQARVPPAFPEPPGTKRRHPGAEVVPADDATKRPKTGVPAAPTRAESPPLS
  ARYGPEAAEGGGDGGRYACYFRDLQTGDASPSPLSAFRGPQRPPYGFGLP
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001798
• ORF Size: 1410 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001798.1, NP_044512
• RefSeq ORF: 1410
• Locus ID: 1487329
• MW: 49.9 kDa