US3 (NC_001798) Virus Tagged ORF Clone

CAT#: VC100928

Myc-DDK-tagged ORF clone of viral ORF for serine/threonine protein kinase US3 [Human herpesvirus 2], codon optimized for human cell expression, NP_044533

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: US3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100928 represents NCBI reference of NP_044533 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTGTCGCAAATTTTGCGGTGTGTACCGCAGACCCGACAAGCGCCAGGAAGCATCCGTTCCGCCCG
  AAACCAACACGGCACCCGCATTCCCTGCTAGCACTTTCTACACACCAGCAGAGGATGCGTATTTGGCCCC
  CGGGCCTCCCGAGACAATTCATCCCTCAAGACCTCCGTCCCCCGGGGAGGCAGCCCGACTGTGCCAGCTC
  CAGGAAATTCTGGCCCAGATGCATTCCGACGAGGATTATCCAATCGTTGATGCTGCCGGCGCCGAGGAGG
  AGGACGAAGCTGACGATGATGCCCCGGACGATGTCGCCTACCCCGAAGACTACGCCGAAGGACGGTTCCT
  GTCAATGGTCTCTGCTGCTCCACTGCCTGGTGCTTCTGGGCACCCACCTGTTCCTGGACGGGCAGCGCCT
  CCTGATGTTAGGACTTGCGATACCGGGAAGGTCGGTGCCACCGGGTTTACTCCGGAAGAGTTGGACACCA
  TGGATCGGGAGGCGCTGCGCGCTATTTCTCGCGGGTGCAAGCCACCCTCTACACTTGCTAAGCTGGTGAC
  CGGGCTCGGATTCGCAATTCATGGTGCACTGATCCCAGGGAGCGAGGGATGCGTCTTTGACAGTTCTCAC
  CCAAACTATCCTCACAGGGTCATTGTGAAGGCCGGGTGGTACGCCAGTACAAGTCATGAAGCAAGGCTGC
  TCCGCAGACTTAATCATCCTGCCATCCTGCCCCTGCTCGACCTGCATGTAGTCTCTGGCGTTACATGTCT
  GGTTCTCCCTAAGTACCACTGCGACTTGTACACGTACCTGAGCAAGCGACCTAGCCCTCTGGGGCACCTG
  CAGATTACCGCCGTGAGCAGGCAGCTCCTGTCCGCGATTGACTACGTCCACTGTAAAGGCATAATCCATA
  GGGATATCAAAACAGAAAATATCTTCATAAACACTCCCGAAAACATTTGCCTCGGAGATTTTGGTGCCGC
  TTGCTTCGTGCGCGGTTGCAGGTCTAGCCCATTCCACTATGGCATTGCCGGAACCATTGATACTAATGCA
  CCCGAGGTTCTGGCCGGCGACCCCTACACCCAGGTGATAGACATTTGGAGCGCGGGCCTTGTTATCTTCG
  AGACTGCCGTGCATACGGCCAGCTTGTTCTCAGCCCCAAGAGATCCAGAGAGGCGGCCTTGTGACAATCA
  AATAGCCAGGATCATCCGGCAGGCGCAGGTGCATGTCGATGAGTTCCCAACACACGCCGAGTCTCGCTTG
  ACGGCCCACTACCGGTCTCGCGCTGCCGGTAATAATCGCCCAGCGTGGACCAGGCCTGCTTGGACCAGGT
  ACTACAAAATCCATACAGACGTGGAGTATCTCATCTGTAAAGCCCTGACCTTTGATGCCGCACTGCGGCC
  CAGTGCAGCTGAGCTGCTGCGACTTCCCCTTTTTCACCCAAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100928 representing NP_044533
  Red=Cloning sites Green=Tags
  
  MACRKFCGVYRRPDKRQEASVPPETNTAPAFPASTFYTPAEDAYLAPGPPETIHPSRPPSPGEAARLCQL
  QEILAQMHSDEDYPIVDAAGAEEEDEADDDAPDDVAYPEDYAEGRFLSMVSAAPLPGASGHPPVPGRAAP
  PDVRTCDTGKVGATGFTPEELDTMDREALRAISRGCKPPSTLAKLVTGLGFAIHGALIPGSEGCVFDSSH
  PNYPHRVIVKAGWYASTSHEARLLRRLNHPAILPLLDLHVVSGVTCLVLPKYHCDLYTYLSKRPSPLGHL
  QITAVSRQLLSAIDYVHCKGIIHRDIKTENIFINTPENICLGDFGAACFVRGCRSSPFHYGIAGTIDTNA
  PEVLAGDPYTQVIDIWSAGLVIFETAVHTASLFSAPRDPERRPCDNQIARIIRQAQVHVDEFPTHAESRL
  TAHYRSRAAGNNRPAWTRPAWTRYYKIHTDVEYLICKALTFDAALRPSAAELLRLPLFHPK
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001798
• ORF Size: 1443 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001798.1, NP_044533
• RefSeq ORF: 1443
• Locus ID: 1487355
• MW: 52.7 kDa