UL10 (NC_001798) Virus Tagged ORF Clone

CAT#: VC100874

Myc-DDK-tagged ORF clone of viral ORF for envelope glycoprotein M [Human herpesvirus 2], codon optimized for human cell expression, NP_044479

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UL10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100874 represents NCBI reference of NP_044479 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTAGAAGAGCTCCACGCGGCAGCCCAGAGGCTGCTCCAGGGGCAGATGTTGCTCCGGGGGCACGCG
  CCGCTTGGTGGGTGTGGTGTGTACAGGTGGCAACCTTCATCGTGAGCGCGATCTGTGTTGTCGGATTGCT
  CGTGCTTGCCTCCGTGTTCAGAGACCGCTTTCCATGTCTCTACGCGCCAGCAACCAGCTACGCTAAAGCC
  AACGCCACAGTGGAGGTGCGAGGGGGGGTGGCCGTGCCTCTTAGACTCGACACACAGTCTCTGTTGGCTA
  CCTATGCCATTACAAGTACCCTGCTGCTCGCCGCCGCCGTATACGCAGCCGTGGGTGCGGTAACATCTAG
  GTATGAGCGCGCCCTGGACGCTGCCAGGCGGCTGGCTGCTGCCAGAATGGCAATGCCTCACGCTACTCTC
  ATCGCTGGTAACGTATGTGCCTGGCTTCTGCAGATTACGGTCCTCCTTCTGGCCCATAGGATCAGCCAAC
  TTGCTCACCTCATTTACGTGCTCCACTTTGCCTGCCTTGTGTATTTGGCTGCTCATTTCTGTACCCGCGG
  GGTACTCTCCGGCACTTACCTTAGGCAGGTCCATGGATTGATAGACCCGGCCCCTACCCACCATCGCATT
  GTGGGACCCGTACGCGCTGTGATGACAAATGCCCTGTTGTTGGGCACTTTGCTCTGCACAGCCGCTGCCG
  CCGTGAGTCTCAATACAATCGCAGCCTTGAACTTTAATTTCTCTGCTCCTTCCATGCTCATTTGCCTGAC
  AACCCTTTTTGCATTGCTTGTGGTATCACTCCTGCTTGTCGTGGAAGGCGTCCTGTGCCACTACGTGCGC
  GTCCTGGTTGGTCCACATCTGGGAGCCATTGCCGCCACAGGCATTGTCGGACTTGCGTGTGAGCACTATC
  ATACTGGAGGATATTATGTAGTCGAGCAGCAATGGCCAGGGGCTCAGACTGGTGTTCGCGTGGCTCTGGC
  GCTTGTGGCAGCATTCGCCCTCGCCATGGCAGTTTTGAGATGCACACGCGCCTATCTCTATCATAGGCGC
  CACCATACAAAGTTCTTTGTACGCATGCGGGATACCAGACATCGCGCCCATAGCGCTTTGAGAAGGGTGC
  GCTCATCTATGAGGGGCTCCCGAAGAGGCGGCCCGCCTGGGGACCCAGGCTACGCAGAAACTCCATATGC
  TTCTGTCTCTCATCACGCTGAAATAGATCGATACGGCGATAGCGATGGAGATCCAATATACGACGAAGTG
  GCACCTGATCACGAGGCAGAACTTTACGCCAGAGTGCAGAGACCCGGACCAGTCCCTGACGCAGAGCCTA
  TTTACGATACGGTTGAGGGGTATGCTCCAAGAAGTGCGGGCGAGCCGGTGTACTCTACAGTGAGGAGGTG
  G
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100874 representing NP_044479
  Red=Cloning sites Green=Tags
  
  MGRRAPRGSPEAAPGADVAPGARAAWWVWCVQVATFIVSAICVVGLLVLASVFRDRFPCLYAPATSYAKA
  NATVEVRGGVAVPLRLDTQSLLATYAITSTLLLAAAVYAAVGAVTSRYERALDAARRLAAARMAMPHATL
  IAGNVCAWLLQITVLLLAHRISQLAHLIYVLHFACLVYLAAHFCTRGVLSGTYLRQVHGLIDPAPTHHRI
  VGPVRAVMTNALLLGTLLCTAAAAVSLNTIAALNFNFSAPSMLICLTTLFALLVVSLLLVVEGVLCHYVR
  VLVGPHLGAIAATGIVGLACEHYHTGGYYVVEQQWPGAQTGVRVALALVAAFALAMAVLRCTRAYLYHRR
  HHTKFFVRMRDTRHRAHSALRRVRSSMRGSRRGGPPGDPGYAETPYASVSHHAEIDRYGDSDGDPIYDEV
  APDHEAELYARVQRPGPVPDAEPIYDTVEGYAPRSAGEPVYSTVRRW
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001798
• ORF Size: 1401 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001798.1, NP_044479
• RefSeq ORF: 1401
• Locus ID: 1487293
• MW: 50.4 kDa