UL22 (NC_001798) Virus Tagged ORF Clone

CAT#: VC100886

Myc-DDK-tagged ORF clone of viral ORF for envelope glycoprotein H [Human herpesvirus 2], codon optimized for human cell expression, NP_044491

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UL22
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100886 represents NCBI reference of NP_044491 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGACCTGGTCTCTGGGTGGTGATGGGGGTGCTGGTGGGAGTGGCAGGAGGGCACGATACTTACTGGA
  CTGAACAGATTGACCCATGGTTTCTCCACGGCCTCGGATTGGCTCGCACATACTGGCGCGATACCAACAC
  CGGACGCCTCTGGCTGCCCAATACACCTGATGCGTCTGACCCTCAGCGGGGGCGACTTGCTCCACCCGGC
  GAGCTGAATCTGACGACTGCTTCAGTCCCCATGCTTAGGTGGTATGCTGAAAGGTTCTGTTTCGTGCTCG
  TCACCACAGCCGAATTTCCTCGAGATCCAGGCCAGCTTTTGTATATCCCTAAAACATATCTGCTGGGTAG
  ACCACGCAATGCGAGCCTTCCGGAGCTCCCGGAGGCCGGCCCCACTAGCCGACCACCAGCCGAGGTTACA
  CAGCTCAAGGGCCTGTCCCACAACCCAGGGGCCTCTGCTCTCCTGAGGTCAAGAGCATGGGTTACATTCG
  CAGCCGCACCGGATCGGGAGGGGCTTACTTTTCCTCGCGGGGACGATGGGGCTACTGAGCGGCACCCAGA
  CGGACGGAGGAATGCACCCCCCCCCGGTCCTCCCGCTGGAACCCCTAGACATCCTACGACTAATCTCAGC
  ATCGCGCATCTCCACAATGCATCTGTCACATGGCTCGCCGCCAGAGGTCTTCTGAGAACCCCAGGGAGAT
  ACGTTTATCTTTCCCCGAGTGCTTCAACATGGCCCGTTGGAGTGTGGACGACTGGAGGCCTGGCCTTTGG
  TTGTGATGCTGCACTGGTGCGGGCCAGGTATGGAAAGGGCTTTATGGGCCTGGTGATCAGCATGAGAGAT
  AGCCCTCCCGCTGAAATCATCGTAGTCCCCGCAGACAAAACCCTCGCCAGAGTCGGTAACCCAACAGATG
  AGAACGCCCCCGCCGTGCTGCCAGGCCCCCCAGCTGGGCCGCGGTACAGGGTGTTTGTGTTGGGTGCCCC
  TACCCCCGCTGATAATGGTAGCGCTCTCGATGCACTCCGACGGGTGGCAGGGTACCCCGAAGAATCCACC
  AATTACGCCCAGTACATGTCAAGGGCTTACGCCGAGTTCCTCGGAGAGGACCCTGGGTCAGGAACGGACG
  CCCGGCCTAGTCTGTTTTGGCGGTTGGCTGGGCTGCTCGCTAGCTCCGGCTTCGCGTTTGTGAATGCAGC
  GCACGCACACGATGCCATACGCCTTTCTGATCTGTTGGGATTCCTGGCTCACAGCCGCGTCCTGGCCGGA
  CTTGCTGCCCGAGGCGCTGCTGGGTGCGCTGCTGACTCCGTATTCCTCAACGTCAGCGTTCTGGACCCAG
  CCGCAAGGCTCCGGCTTGAAGCACGACTGGGACACCTCGTCGCCGCAATCCTCGAGCGGGAGCAGAGCCT
  CGTCGCTCATGCACTCGGTTACCAGCTTGCGTTTGTGCTGGATAGCCCTGCCGCATACGGCGCTGTAGCG
  CCATCCGCAGCCAGACTTATCGACGCCCTGTACGCCGAATTTCTGGGTGGCCGCGCCCTGACTGCTCCCA
  TGGTGCGCCGCGCACTCTTTTACGCGACCGCAGTTCTGAGAGCACCTTTTCTGGCTGGAGCACCCAGCGC
  TGAACAGAGAGAGAGGGCCAGGCGAGGACTGCTGATCACCACTGCTCTGTGTACTTCTGATGTTGCAGCC
  GCCACCCATGCTGATTTGAGAGCGGCACTTGCGAGGACCGACCACCAGAAGAACCTGTTCTGGCTTCCGG
  ACCACTTCTCACCTTGCGCAGCCTCCCTTCGCTTCGATTTGGCCGAGGGTGGCTTCATTCTGGATGCCCT
  GGCTATGGCGACCAGGTCCGACATACCAGCCGACGTGATGGCACAGCAGACAAGGGGGGTAGCTTCAGTG
  TTGACAAGGTGGGCGCATTACAACGCTCTGATCCGCGCATTCGTACCCGAGGCTACCCATCAGTGTTCCG
  GCCCTAGTCACAATGCCGAGCCCAGGATTCTCGTGCCTATTACGCATAATGCCTCTTATGTGGTGACACA
  CACACCACTGCCCCGGGGCATCGGTTACAAACTGACTGGAGTCGACGTGAGAAGACCACTTTTCATTACA
  TACCTTACAGCCACCTGCGAGGGGCACGCGCGCGAAATAGAACCCAAGAGGTTGGTGAGAACCGAAAACC
  GACGGGACCTGGGACTGGTGGGGGCCGTCTTTCTTAGGTACACCCCGGCCGGAGAGGTGATGAGCGTGCT
  CTTGGTTGACACTGACGCAACACAGCAGCAGCTGGCACAGGGACCAGTGGCGGGAACACCTAATGTGTTT
  AGCTCCGACGTCCCGTCCGTGGCCCTGCTTTTGTTCCCTAACGGAACAGTAATCCACTTGCTCGCATTCG
  ATACACTGCCTATAGCCACGATCGCCCCCGGGTTTCTGGCCGCCTCAGCACTCGGGGTAGTGATGATCAC
  CGCGGCTCTCGCAGGCATTCTCCGCGTAGTGAGGACATGTGTCCCCTTTCTGTGGCGCCGAGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100886 representing NP_044491
  Red=Cloning sites Green=Tags
  
  MGPGLWVVMGVLVGVAGGHDTYWTEQIDPWFLHGLGLARTYWRDTNTGRLWLPNTPDASDPQRGRLAPPG
  ELNLTTASVPMLRWYAERFCFVLVTTAEFPRDPGQLLYIPKTYLLGRPRNASLPELPEAGPTSRPPAEVT
  QLKGLSHNPGASALLRSRAWVTFAAAPDREGLTFPRGDDGATERHPDGRRNAPPPGPPAGTPRHPTTNLS
  IAHLHNASVTWLAARGLLRTPGRYVYLSPSASTWPVGVWTTGGLAFGCDAALVRARYGKGFMGLVISMRD
  SPPAEIIVVPADKTLARVGNPTDENAPAVLPGPPAGPRYRVFVLGAPTPADNGSALDALRRVAGYPEEST
  NYAQYMSRAYAEFLGEDPGSGTDARPSLFWRLAGLLASSGFAFVNAAHAHDAIRLSDLLGFLAHSRVLAG
  LAARGAAGCAADSVFLNVSVLDPAARLRLEARLGHLVAAILEREQSLVAHALGYQLAFVLDSPAAYGAVA
  PSAARLIDALYAEFLGGRALTAPMVRRALFYATAVLRAPFLAGAPSAEQRERARRGLLITTALCTSDVAA
  ATHADLRAALARTDHQKNLFWLPDHFSPCAASLRFDLAEGGFILDALAMATRSDIPADVMAQQTRGVASV
  LTRWAHYNALIRAFVPEATHQCSGPSHNAEPRILVPITHNASYVVTHTPLPRGIGYKLTGVDVRRPLFIT
  YLTATCEGHAREIEPKRLVRTENRRDLGLVGAVFLRYTPAGEVMSVLLVDTDATQQQLAQGPVAGTPNVF
  SSDVPSVALLLFPNGTVIHLLAFDTLPIATIAPGFLAASALGVVMITAALAGILRVVRTCVPFLWRRE
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001798
• ORF Size: 2514 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001798.1, NP_044491
• RefSeq ORF: 2514
• Locus ID: 1487306
• MW: 89.7 kDa