US8 (NC_001798) Virus Tagged ORF Clone

CAT#: VC100933

Myc-DDK-tagged ORF clone of viral ORF for envelope glycoprotein E [Human herpesvirus 2], codon optimized for human cell expression, NP_044538

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: US8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100933 represents NCBI reference of NP_044538 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGGGGAGCAGGGTTGGTCTTCTTCGTGGGCGTGTGGGTGGTTTCATGTCTGGCTGCCGCCCCAC
  GCACAAGCTGGAAGAGGGTGACATCCGGAGAAGACGTCGTGTTGCTGCCAGCTCCCGCGGAGCGGACGAG
  GGCGCATAAGCTGCTTTGGGCGGCTGAGCCATTGGATGCATGTGGCCCTCTGAGGCCATCTTGGGTCGCT
  CTGTGGCCCCCAAGGCGCGTGTTGGAGACAGTGGTTGATGCCGCTTGTATGAGAGCACCAGAACCTCTGG
  CCATTGCCTATTCCCCGCCCTTCCCCGCCGGGGACGAGGGCCTTTATAGCGAATTGGCGTGGCGGGATAG
  AGTTGCAGTCGTCAATGAGAGTCTCGTGATATATGGCGCCCTTGAGACCGATAGCGGTCTGTACACACTG
  TCCGTCGTGGGCCTGTCAGATGAGGCCCGCCAAGTGGCAAGCGTTGTTCTGGTGGTTGAGCCTGCCCCTG
  TGCCTACGCCCACCCCTGACGACTATGATGAGGAAGACGACGCCGGAGTGACCAATGCGCGCAGGAGCGC
  GTTCCCACCACAGCCCCCCCCAAGAAGACCTCCTGTAGCCCCCCCTACCCACCCCAGGGTCATCCCTGAA
  GTTTCTCATGTGCGGGGTGTTACTGTGCACATGGAAACACTCGAAGCAATTCTCTTTGCGCCCGGCGAGA
  CCTTTGGCACAAACGTGTCCATCCACGCCATCGCTCACGACGACGGACCATACGCGATGGATGTAGTTTG
  GATGCGCTTTGATGTCCCCAGCAGCTGTGCCGATATGAGAATATATGAAGCCTGTCTGTACCACCCACAG
  CTGCCGGAGTGCCTGAGCCCTGCAGATGCACCATGCGCCGTCAGTTCTTGGGCTTACCGCTTGGCTGTTC
  GATCCTATGCTGGATGTTCAAGGACAACCCCGCCACCCCGCTGTTTTGCTGAAGCACGCATGGAACCAGT
  TCCCGGCCTGGCGTGGCTCGCAAGCACGGTCAATCTGGAGTTCCAGCACGCCTCACCTCAGCACGCAGGG
  CTGTACCTGTGCGTGGTCTATGTCGACGACCACATCCATGCCTGGGGGCATATGACCATCAGTACTGCCG
  CACAATATCGAAATGCCGTCGTAGAACAGCACCTGCCCCAAAGGCAGCCTGAGCCGGTCGAGCCTACACG
  CCCCCATGTCAGGGCTCCCCACCCAGCCCCTTCTGCACGCGGGCCACTCAGATTGGGTGCTGTATTGGGA
  GCTGCCCTTCTCTTGGCGGCTCTCGGATTGAGCGCATGGGCCTGCATGACATGCTGGCGAAGAAGGTCAT
  GGAGAGCCGTTAAGTCCCGAGCCAGTGCCACAGGGCCAACGTACATCAGGGTTGCAGATTCCGAGCTCTA
  CGCTGACTGGTCCAGCGACTCCGAGGGAGAACGGGACGGGAGCCTCTGGCAGGACCCTCCCGAACGCCCT
  GACTCCCCCAGTACAAACGGATCAGGATTCGAAATCCTGAGTCCAACCGCTCCCTCCGTATATCCTCACT
  CTGAAGGTCGGAAGAGCCGGCGGCCCCTGACTACGTTCGGGAGTGGGAGCCCCGGGAGACGACACAGTCA
  GGCTAGTTACCCCAGCGTCCTCTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100933 representing NP_044538
  Red=Cloning sites Green=Tags
  
  MARGAGLVFFVGVWVVSCLAAAPRTSWKRVTSGEDVVLLPAPAERTRAHKLLWAAEPLDACGPLRPSWVA
  LWPPRRVLETVVDAACMRAPEPLAIAYSPPFPAGDEGLYSELAWRDRVAVVNESLVIYGALETDSGLYTL
  SVVGLSDEARQVASVVLVVEPAPVPTPTPDDYDEEDDAGVTNARRSAFPPQPPPRRPPVAPPTHPRVIPE
  VSHVRGVTVHMETLEAILFAPGETFGTNVSIHAIAHDDGPYAMDVVWMRFDVPSSCADMRIYEACLYHPQ
  LPECLSPADAPCAVSSWAYRLAVRSYAGCSRTTPPPRCFAEARMEPVPGLAWLASTVNLEFQHASPQHAG
  LYLCVVYVDDHIHAWGHMTISTAAQYRNAVVEQHLPQRQPEPVEPTRPHVRAPHPAPSARGPLRLGAVLG
  AALLLAALGLSAWACMTCWRRRSWRAVKSRASATGPTYIRVADSELYADWSSDSEGERDGSLWQDPPERP
  DSPSTNGSGFEILSPTAPSVYPHSEGRKSRRPLTTFGSGSPGRRHSQASYPSVLW
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001798
• ORF Size: 1635 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001798.1, NP_044538
• RefSeq ORF: 1635
• Locus ID: 1487360
• MW: 59.3 kDa