US10 (NC_001798) Virus Tagged ORF Clone

CAT#: VC100936

Myc-DDK-tagged ORF clone of viral ORF for virion protein US10 [Human herpesvirus 2], codon optimized for human cell expression, NP_044541

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: US10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100936 represents NCBI reference of NP_044541 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTAGGAGGCGAGGCAACGTGGAAATTAGAGTCTATTACGAATCAGTGCGCCCTTCACGATCTAGGA
  GTCACTTGAAGCCGTCTGACCACCAAGAATTCCCTGGTCACCACGTGAGTCCCGGATCTCCCGGCTTTCC
  CGAAAGTCCGGGGAACAGGGAATTTCACGACCTCCCAGAAAATCCAGGTAGCAGAGCCTATCCTGGCACC
  CGCGACCCCCATGACCCCCATGGTTGTCCTGGATCACTCGACCCACACGGCAACCCAGCCCAACCCGCAG
  GCCTGCCATCTCCTGTACCTTACGCACCCCTGGGAAGCCCCGACCCTTCTTCACCCCGGCAGCGCACCTA
  CGTCCTTCCACGAGTCGGAATACATAATGCACCAGCAAGTGACACCAGAGCGCCCAAGAGAGCCAACAGT
  AGACACCGGGCGGACCGACCACCGGAGTCCCCCGGATCCGAGCTGTACCCCCTCAACGCACAGGCACTGG
  CTCATCTGCAGATGCTGCCGGCCGATCATAGAGCTTTTTTCAGAACTGTCATCGAGGTGAGCCGCTTGTG
  CGCTCTTAACACACACGACCCCCCTCCTCCTCTTGCTGGCGCCCGCGTCGGGCAGGAGGCACAGTTGGTA
  CACACCCAGTGGCTCCGAGCCAACAGGGAATCTAGCCCCCTGTGGCCATGGCGCACCGCCGCAATGAATT
  TCATCGCTGCCGCTGCCCCTTGTGTGCAAACGCACAGACACATGCATGATCTTCTTATGGCATGTGCCTT
  TTGGTGTTGTCTTGCACACGCATCAACATGCTCATACGCCGGATTGTATAGTGCCCATTGTCAGCATTTG
  TTCAGAGCCTTCGGCTGCGGCCCTCCCGTGCTCACAACGTCCCGCGGCCAGGGCGGATGGTGCAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100936 representing NP_044541
  Red=Cloning sites Green=Tags
  
  MIRRRGNVEIRVYYESVRPSRSRSHLKPSDHQEFPGHHVSPGSPGFPESPGNREFHDLPENPGSRAYPGT
  RDPHDPHGCPGSLDPHGNPAQPAGLPSPVPYAPLGSPDPSSPRQRTYVLPRVGIHNAPASDTRAPKRANS
  RHRADRPPESPGSELYPLNAQALAHLQMLPADHRAFFRTVIEVSRLCALNTHDPPPPLAGARVGQEAQLV
  HTQWLRANRESSPLWPWRTAAMNFIAAAAPCVQTHRHMHDLLMACAFWCCLAHASTCSYAGLYSAHCQHL
  FRAFGCGPPVLTTSRGQGGWCN
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001798
• ORF Size: 906 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001798.1, NP_044541
• RefSeq ORF: 906
• Locus ID: 1487351
• MW: 33.2 kDa