US1 (NC_001798) Virus Tagged ORF Clone

CAT#: VC100926

Myc-DDK-tagged ORF clone of viral ORF for regulatory protein ICP22 [Human herpesvirus 2], codon optimized for human cell expression, NP_044531

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: US1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100926 represents NCBI reference of NP_044531 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGACATCCCCCCAGATCCCCCCGCCCTGAATACCACACCGGCAAATCATGCACCACCAAGTCCAC
  CACCGGGTTCCAGAAAGCGAAGGCGGCCCGTGTTGCCATCTAGCAGTGAATCCGAGGGCAAACCCGACAC
  TGAATCCGAGTCTAGCAGCACCGAAAGCTCTGAGGATGAGGCCGGCGATCTGCGCGGCGGCAGACGCAGA
  TCCCCGCGGGAGCTGGGCGGACGCTATTTTCTCGATCTCTCCGCGGAGTCTACAACGGGGACTGAGTCCG
  AAGGGACTGGCCCCTCAGATGACGATGACGACGATGCCTCTGACGGCTGGCTCGTTGATACACCCCCCCG
  GAAAAGCAAGCGGCCTCGGATAAATCTGCGCCTTACTTCCTCTCCCGACAGGCGAGCCGGTGTGGTGTTC
  CCTGAGGTCTGGCGGTCCGACCGGCCTATTCGAGCCGCCCAACCTCAGGCACCGGCTAGTCTGCCAGGAA
  TCGCTCATGCCCATCGGCGGTCCGCGAGGCAAGCCCAGATGCGATCTGGAGCAGCCTGGACACTGGACCT
  GCATTATATTAGACAATGCGTGAACCAGCTCTTTCGCATTCTGAGAGCAGCTCCCAATCCGCCAGGCTCA
  GCTAATCGGTTGAGACACCTGGTGCGCGATTGCTACCTCATGGGGTATTGTAGGACAAGACTGGGACCGC
  GCACTTGGGGGCGGTTGCTCCAAATAAGCGGCGGAACTTGGGACGTGAGGCTGCGGAATGCCATTCGGGA
  AGTGGAAGCTCACTTTGAACCTGCAGCAGAGCCGGTCTGCGAGCTCCCATGTTTGAATGCTCGGAGGTAT
  GGACCAGAGTGCGACGTTGGAAATCTGGAGACCAACGGTGGCAGTACCAGCGATGACGAGATTAGTGACG
  CAACCGATTCTGATGATACCCTGGCTAGTCACTCAGACACTGAAGGGGGACCATCTCCAGCGGGGAGGGA
  GAACCCAGAGTCCGCATCCGGTGGCGCTATCGCAGCCAGACTGGAGTGCGAGTTCGGCACTTTCGATTGG
  ACGTCCGAGGAGGGGTCCCAGCCATGGCTCAGTGCAGTTGTCGCGGATACGAGTAGCGCAGAGCGGTCCG
  GTCTGCCGGCCCCCGGAGCCTGTAGGGCCACTGAAGCTCCCGAACGGGAGGATGGTTGCAGGAAAATGAG
  GTTCCCTGCAGCATGTCCTTACCCATGCGGACATACATTCCTGAGACCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100926 representing NP_044531
  Red=Cloning sites Green=Tags
  
  MADIPPDPPALNTTPANHAPPSPPPGSRKRRRPVLPSSSESEGKPDTESESSSTESSEDEAGDLRGGRRR
  SPRELGGRYFLDLSAESTTGTESEGTGPSDDDDDDASDGWLVDTPPRKSKRPRINLRLTSSPDRRAGVVF
  PEVWRSDRPIRAAQPQAPASLPGIAHAHRRSARQAQMRSGAAWTLDLHYIRQCVNQLFRILRAAPNPPGS
  ANRLRHLVRDCYLMGYCRTRLGPRTWGRLLQISGGTWDVRLRNAIREVEAHFEPAAEPVCELPCLNARRY
  GPECDVGNLETNGGSTSDDEISDATDSDDTLASHSDTEGGPSPAGRENPESASGGAIAARLECEFGTFDW
  TSEEGSQPWLSAVVADTSSAERSGLPAPGACRATEAPEREDGCRKMRFPAACPYPCGHTFLRP
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001798
• ORF Size: 1239 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001798.1, NP_044531
• RefSeq ORF: 1239
• Locus ID: 1487350
• MW: 44.7 kDa