US8A (NC_001798) Virus Tagged ORF Clone

CAT#: VC100934

Myc-DDK-tagged ORF clone of viral ORF for membrane protein US8A [Human herpesvirus 2], codon optimized for human cell expression, NP_044539

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: US8A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100934 represents NCBI reference of NP_044539 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCCCGCGCTGAGGAGTTATCACCAGCGGCTTCGCCTTTACACTCCAATCGCTCGAGGCGTGAATC
  TGGCAGCCAGAAGCCCTCCACTTGTGAGGGAGGCACGGGCAGTTGTCACTCCAAGACCTCCTATCCGCCC
  CTCATCAGGGAAGGCCAGCAGCGACGATGCCGATGTGGGGGACGAGCTGATTGCTATCGCTGATGCTAGA
  GGAGACCCTCCTGAGACCCTTCCTCCAGGTGCTGGAGGAGCAGCACCTGCGTGCAGGCGACCACCGCGGG
  GAGGATCACCAGCCGCCTTTCCAGTAGCTCTTCACGCCGTGGACGCTCCGAGCCAGTTTGTGACATGGTT
  GGCTGTGCGGTGGCTGAGAGGCGCTGTAGGTCTGGGCGCCGTCCTGTGCGGTATTGCTTTTTACGTAACG
  TCCATCGCTAGAGGCGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100934 representing NP_044539
  Red=Cloning sites Green=Tags
  
  MDPALRSYHQRLRLYTPIARGVNLAARSPPLVREARAVVTPRPPIRPSSGKASSDDADVGDELIAIADAR
  GDPPETLPPGAGGAAPACRRPPRGGSPAAFPVALHAVDAPSQFVTWLAVRWLRGAVGLGAVLCGIAFYVT
  SIARGA
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001798
• ORF Size: 438 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001798.1, NP_044539
• RefSeq ORF: 438
• Locus ID: 1487361
• MW: 15.1 kDa