UL30 (NC_001798) Virus Tagged ORF Clone

CAT#: VC100895

Myc-DDK-tagged ORF clone of viral ORF for DNA polymerase catalytic subunit [Human herpesvirus 2], codon optimized for human cell expression, NP_044500

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UL30
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100895 represents NCBI reference of NP_044500 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCTGTGCAGCCGGCGGACCTGCCTCCCCTGGCGGAAAACCCGCAGCACGCGCAGCCTCCGGGTTTT
  TTGCACCACACAACCCAAGAGGAGCTACACAGACCGCCCCCCCGCCATGTCGCAGGCAGAATTTCTACAA
  TCCGCACCTCGCCCAGACCGGCACCCAACCCAAAGCGCTCGGTCCAGCCCAGAGGCATACCTACTACTCC
  GAGTGTGATGAATTTAGGTTTATCGCCCCTCGGAGCCTCGATGAGGACGCCCCTGCTGAACAGAGAACCG
  GCGTCCACGACGGCCGCCTGCGGAGAGCTCCTAAAGTGTACTGCGGGGGCGATGAGCGGGATGTCTTGCG
  GGTGGGGCCAGAGGGCTTTTGGCCACGCAGGCTCCGGTTGTGGGGAGGCGCTGACCACGCTCCAGAAGGC
  TTCGACCCCACAGTCACTGTTTTTCACGTTTATGATATACTCGAGCACGTGGAACACGCTTATTCTATGC
  GGGCTGCACAATTGCACGAGCGCTTCATGGACGCAATCACTCCAGCCGGCACAGTCATCACTCTGCTCGG
  ACTTACTCCAGAGGGCCATCGCGTGGCGGTGCACGTGTACGGTACCCGCCAGTATTTTTATATGAATAAA
  GCGGAGGTCGATCGGCACCTGCAATGTAGAGCCCCAAGAGATTTGTGTGAGCGGCTGGCAGCAGCACTCA
  GAGAGTCACCTGGCGCTTCTTTCCGCGGCATTAGCGCTGACCACTTTGAAGCAGAGGTGGTGGAAAGGGC
  TGACGTGTACTACTACGAAACCAGGCCAACACTGTACTACAGAGTGTTCGTGAGATCAGGACGCGCCCTG
  GCTTACCTCTGCGACAACTTTTGCCCCGCTATTAGAAAGTATGAAGGGGGTGTGGATGCCACAACCCGCT
  TTATCCTCGATAACCCTGGATTCGTCACATTTGGATGGTATCGCCTGAAACCTGGAAGAGGCAACGCTCC
  TGCCCAGCCAAGGCCTCCTACAGCTTTTGGCACCAGTTCCGATGTCGAATTTAATTGCACCGCCGATAAC
  CTGGCAGTCGAAGGAGCTATGTGCGATCTCCCGGCATATAAGCTTATGTGCTTCGATATAGAATGCAAAG
  CAGGTGGCGAAGACGAACTGGCTTTCCCGGTGGCCGAGCGACCAGAGGATCTCGTGATTCAGATCAGCTG
  TTTGCTGTACGACCTCTCAACTACCGCACTGGAACATATACTCCTGTTCAGCCTCGGATCCTGTGATCTT
  CCCGAATCCCACCTGAGCGACCTCGCATCCCGGGGCTTGCCCGCACCCGTGGTGCTCGAATTCGACTCTG
  AATTTGAAATGCTGCTGGCATTTATGACCTTTGTCAAACAATACGGACCAGAGTTTGTAACCGGGTATAA
  TATCATCAATTTCGATTGGCCTTTTGTACTGACTAAGCTGACAGAGATCTACAAAGTGCCACTCGATGGC
  TACGGAAGGATGAACGGGCGAGGAGTATTCCGAGTCTGGGATATCGGCCAGAGCCATTTTCAGAAAAGGT
  CAAAAATCAAAGTTAACGGAATGGTCAATATTGATATGTACGGGATTATAACAGACAAGGTGAAGCTGAG
  TAGCTATAAGCTCAACGCCGTGGCCGAGGCAGTTCTCAAAGATAAGAAAAAGGATCTCAGCTATAGAGAC
  ATTCCTGCGTACTATGCTTCCGGCCCCGCTCAAAGAGGCGTTATCGGCGAATATTGTGTTCAAGACAGCC
  TCCTCGTCGGCCAGCTGTTCTTCAAATTCCTGCCCCACCTGGAGTTGAGTGCCGTTGCACGCCTCGCTGG
  TATCAATATAACACGAACCATCTATGACGGCCAACAGATTAGAGTTTTTACCTGTCTGCTCAGGCTGGCC
  GGTCAAAAAGGTTTTATACTTCCCGACACGCAAGGCCGCTTCCGAGGGCTCGATAAGGAAGCTCCTAAAA
  GGCCAGCTGTCCCCAGGGGGGAAGGGGAAAGGCCTGGCGATGGCAATGGAGATGAAGACAAGGACGACGA
  CGAAGACGGGGATGAGGACGGCGATGAAAGAGAAGAGGTAGCCAGGGAGACTGGAGGCAGGCATGTTGGA
  TATCAAGGCGCGAGGGTACTGGACCCCACATCTGGCTTCCATGTTGACCCTGTGGTGGTCTTCGATTTTG
  CTAGCCTGTACCCATCCATTATCCAGGCACACAACCTTTGTTTCTCTACGCTCTCTCTGAGGCCAGAAGC
  TGTAGCACATCTTGAGGCAGATAGGGATTACCTGGAGATAGAGGTAGGGGGCCGCCGCCTCTTTTTCGTG
  AAGGCCCATGTAAGAGAAAGCCTGCTGAGCATTCTCTTGAGAGACTGGCTCGCTATGCGCAAACAGATCC
  GCAGCCGAATCCCTCAATCCACCCCAGAAGAAGCGGTACTGTTGGACAAACAGCAAGCTGCTATCAAAGT
  AGTCTGCAACTCTGTATACGGGTTTACCGGCGTCCAACACGGCCTCCTGCCCTGCTTGCACGTGGCTGCC
  ACTGTGACAACAATTGGACGGGAAATGCTCCTTGCAACGCGGGCCTACGTACACGCCAGGTGGGCTGAAT
  TCGACCAGCTTCTTGCCGATTTCCCAGAAGCCGCTGGCATGCGCGCTCCTGGGCCGTACAGTATGCGCAT
  TATTTACGGGGATACGGACTCCATTTTCGTGCTGTGTAGGGGACTCACTGCCGCTGGGCTCGTCGCCATG
  GGCGACAAGATGGCTAGTCACATCAGTAGGGCTCTTTTCCTCCCACCAATCAAGTTGGAGTGTGAGAAAA
  CTTTTACGAAACTCCTGTTGATTGCAAAAAAGAAGTATATTGGGGTCATCTGTGGAGGCAAAATGCTGAT
  CAAGGGCGTGGACCTCGTGAGAAAAAACAACTGTGCATTTATCAACAGGACCTCCCGGGCGCTCGTCGAT
  CTTCTCTTCTACGACGACACCGTCTCTGGTGCCGCCGCCGCCCTGGCTGAAAGACCCGCAGAGGAGTGGT
  TGGCCAGGCCCTTGCCTGAGGGCCTGCAAGCGTTCGGGGCTGTCCTTGTAGATGCTCATCGGCGCATTAC
  AGATCCAGAAAGAGACATCCAGGACTTTGTTCTCACTGCGGAACTGAGTAGACACCCACGCGCCTATACA
  AATAAGAGACTTGCCCATCTTACCGTGTACTATAAGCTGATGGCCCGCCGAGCGCAGGTACCTTCCATTA
  AGGATCGGATCCCTTATGTCATTGTCGCGCAGACACGCGAAGTAGAGGAAACAGTAGCCCGGCTGGCTGC
  CTTGCGGGAACTTGATGCCGCTGCACCAGGAGACGAGCCAGCTCCACCTGCGGCTCTCCCCTCTCCAGCA
  AAGAGACCTAGAGAGACCCCCTCTCATGCAGATCCTCCAGGTGGTGCATCTAAACCCAGGAAGCTGCTGG
  TTTCTGAGCTGGCTGAAGATCCTGGGTACGCCATCGCCCGGGGCGTCCCGCTGAACACTGACTACTACTT
  TAGTCACCTGTTGGGGGCTGCATGTGTGACCTTCAAAGCTCTGTTCGGAAACAATGCCAAGATTACGGAG
  TCTCTTTTGAAGAGGTTCATTCCAGAGACCTGGCACCCTCCAGATGATGTAGCAGCCAGACTCAGGGCAG
  CAGGGTTCGGACCCGCAGGAGCGGGCGCGACCGCCGAAGAGACTAGACGCATGCTGCACAGGGCATTCGA
  CACTTTGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100895 representing NP_044500
  Red=Cloning sites Green=Tags
  
  MFCAAGGPASPGGKPAARAASGFFAPHNPRGATQTAPPPCRRQNFYNPHLAQTGTQPKALGPAQRHTYYS
  ECDEFRFIAPRSLDEDAPAEQRTGVHDGRLRRAPKVYCGGDERDVLRVGPEGFWPRRLRLWGGADHAPEG
  FDPTVTVFHVYDILEHVEHAYSMRAAQLHERFMDAITPAGTVITLLGLTPEGHRVAVHVYGTRQYFYMNK
  AEVDRHLQCRAPRDLCERLAAALRESPGASFRGISADHFEAEVVERADVYYYETRPTLYYRVFVRSGRAL
  AYLCDNFCPAIRKYEGGVDATTRFILDNPGFVTFGWYRLKPGRGNAPAQPRPPTAFGTSSDVEFNCTADN
  LAVEGAMCDLPAYKLMCFDIECKAGGEDELAFPVAERPEDLVIQISCLLYDLSTTALEHILLFSLGSCDL
  PESHLSDLASRGLPAPVVLEFDSEFEMLLAFMTFVKQYGPEFVTGYNIINFDWPFVLTKLTEIYKVPLDG
  YGRMNGRGVFRVWDIGQSHFQKRSKIKVNGMVNIDMYGIITDKVKLSSYKLNAVAEAVLKDKKKDLSYRD
  IPAYYASGPAQRGVIGEYCVQDSLLVGQLFFKFLPHLELSAVARLAGINITRTIYDGQQIRVFTCLLRLA
  GQKGFILPDTQGRFRGLDKEAPKRPAVPRGEGERPGDGNGDEDKDDDEDGDEDGDEREEVARETGGRHVG
  YQGARVLDPTSGFHVDPVVVFDFASLYPSIIQAHNLCFSTLSLRPEAVAHLEADRDYLEIEVGGRRLFFV
  KAHVRESLLSILLRDWLAMRKQIRSRIPQSTPEEAVLLDKQQAAIKVVCNSVYGFTGVQHGLLPCLHVAA
  TVTTIGREMLLATRAYVHARWAEFDQLLADFPEAAGMRAPGPYSMRIIYGDTDSIFVLCRGLTAAGLVAM
  GDKMASHISRALFLPPIKLECEKTFTKLLLIAKKKYIGVICGGKMLIKGVDLVRKNNCAFINRTSRALVD
  LLFYDDTVSGAAAALAERPAEEWLARPLPEGLQAFGAVLVDAHRRITDPERDIQDFVLTAELSRHPRAYT
  NKRLAHLTVYYKLMARRAQVPSIKDRIPYVIVAQTREVEETVARLAALRELDAAAPGDEPAPPAALPSPA
  KRPRETPSHADPPGGASKPRKLLVSELAEDPGYAIARGVPLNTDYYFSHLLGAACVTFKALFGNNAKITE
  SLLKRFIPETWHPPDDVAARLRAAGFGPAGAGATAEETRRMLHRAFDTLA
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001798
• ORF Size: 3720 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001798.1, NP_044500
• RefSeq ORF: 3720
• Locus ID: 1487316
• MW: 137.3 kDa