BORF1 (NC_007605) Virus Tagged ORF Clone

CAT#: VC101116

Myc-DDK-tagged ORF clone of viral ORF for BORF1 [Human herpesvirus 4], codon optimized for human cell expression, YP_401654

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BORF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101116 represents NCBI reference of YP_401654 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAGTGCAGGGCAGCGTGGATAGGAGGCGCTTGCAGAGGCGAATAGCAGGGTTGCTCCCCCCCCCGG
  CACGGCGCCTGAACATATCTAGGGGCTCTGAATTTACACGGGACGTTAGGGGCCTGGTGGAGGAGCACGC
  ACAAGCCAGCTCTTTGAGTGCCGCCGCTGTCTGGAGGGCCGGACTGCTTGCTCCAGGAGAAGTGGCAGTG
  GCCGGCGGAGGCTCTGGTGGCGGTTCCTTCTCTTGGTCAGGCTGGAGACCCCCCGTGTTTGGGGATTTTC
  TCATCCACGCTTCTTCCTTCAACAATGCCGAAGCCACAGGCACCCCTCTCTTTCAGTTTAAACAATCCGA
  TCCTTTTTCTGGCGTCGATGCTGTCTTTACCCCGCTCAGTCTGTTCATACTTATGAACCATGGCAGGGGA
  GTGGCAGCCAGAGTGGAGGCCGGCGGGGGCCTGACGCGCATGGCTAACTTGCTGTACGACTCACCTGCAA
  CTCTTGCAGACCTCGTCCCGGACTTCGGGCGGCTTGTCGCCGATCGGCGATTCCATAATTTCATCACACC
  AGTGGGGCCTCTGGTCGAGAATATTAAATCAACCTACCTCAACAAAATTACTACGGTCGTCCATGGCCCT
  GTGGTATCCAAAGCAATACCAAGATCAACCGTGAAGGTGACTGTGCCCCAAGAAGCCTTTGTGGATCTGG
  ACGCCTGGTTGTCAGGTGGGGCGGGAGGCGGGGGGGGAGTTTGTTTTGTAGGGGGTCTGGGGTTGCAGCC
  CTGTCCTGCCGACGCCAGACTGTACGTTGCCCTCACATACGAGGAAGCTGGTCCTCGATTTACTTTTTTT
  CAATCCAGCCGCGGCCACTGCCAGATCATGAACATTCTCAGAATTTACTACTCTCCTAGTATCATGCACA
  GATATGCGGTGGTTCAGCCTCTGCATATCGAGGAGCTCACATTTGGTGCAGTGGCCTGTCTTGGTACATT
  CTCAGCCACCGACGGTTGGCGCCGGAGCGCCTTTAACTATCGGGGTAGCAGTCTCCCTGTCGTGGAGATC
  GATTCCTTTTATTCTAACGTTTCAGACTGGGAGGTCATCCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101116 representing YP_401654
  Red=Cloning sites Green=Tags
  
  MKVQGSVDRRRLQRRIAGLLPPPARRLNISRGSEFTRDVRGLVEEHAQASSLSAAAVWRAGLLAPGEVAV
  AGGGSGGGSFSWSGWRPPVFGDFLIHASSFNNAEATGTPLFQFKQSDPFSGVDAVFTPLSLFILMNHGRG
  VAARVEAGGGLTRMANLLYDSPATLADLVPDFGRLVADRRFHNFITPVGPLVENIKSTYLNKITTVVHGP
  VVSKAIPRSTVKVTVPQEAFVDLDAWLSGGAGGGGGVCFVGGLGLQPCPADARLYVALTYEEAGPRFTFF
  QSSRGHCQIMNILRIYYSPSIMHRYAVVQPLHIEELTFGAVACLGTFSATDGWRRSAFNYRGSSLPVVEI
  DSFYSNVSDWEVIL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007605
• ORF Size: 1092 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_007605.1, YP_401654
• RefSeq ORF: 1092
• Locus ID: 3783724
• MW: 39.2 kDa