BRLF1 (NC_007605) Virus Tagged ORF Clone

CAT#: VC101134

Myc-DDK-tagged ORF clone of viral ORF for BRLF1 [Human herpesvirus 4], codon optimized for human cell expression, YP_401674

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BRLF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101134 represents NCBI reference of YP_401674 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGACCAAAAAAAGACGGTTTGGAGGATTTCCTTCGACTCACTCCTGAGATCAAGAAACAGCTGGGAT
  CCCTCGTTTCTGACTACTGTAATGTTCTGAATAAGGAGTTTACAGCCGGATCTGTGGAGATCACACTGCG
  CTCCTACAAGATCTGCAAGGCCTTCATTAATGAGGCGAAAGCTCACGGTAGGGAGTGGGGTGGTCTCATG
  GCTACTCTCAACATTTGCAACTTTTGGGCCATACTTAGAAATAATCGGGTCAGGAGAAGGGCCGAGAACG
  CCGGTAACGACGCTTGCTCAATAGCCTGTCCCATTGTGATGCGCTACGTGCTTGATCATCTCATTGTGGT
  CACCGACAGATTTTTCATCCAGGCGCCAAGTAATAGGGTCATGATCCCCGCGACTATCGGTACCGCCATG
  TACAAACTCCTCAAACACTCTCGAGTTCGGGCCTATACCTACAGCAAGGTGTTGGGTGTGGACAGGGCCG
  CCATTATGGCGAGCGGAAAGCAAGTTGTTGAGCACCTGAACCGGATGGAAAAGGAAGGCCTCTTGAGCTC
  TAAGTTCAAGGCATTCTGCAAGTGGGTGTTTACCTACCCTGTGCTGGAGGAGATGTTCCAGACCATGGTG
  TCTAGTAAGACTGGACACCTTACAGATGATGTGAAAGACGTTCGAGCGCTCATCAAGACACTTCCACGAG
  CCTCCTATTCCTCACACGCAGGCCAGCGCAGCTACGTCAGTGGAGTCCTCCCGGCATGTCTGCTTTCAAC
  AAAATCTAAGGCTGTGGAGACACCTATCCTGGTTTCTGGCGCAGACCGCATGGACGAGGAACTTATGGGA
  AATGATGGGGGCGCATCTCACACAGAGGCCCGGTACTCAGAAAGCGGACAATTTCATGCTTTTACCGACG
  AACTGGAGAGTCTGCCATCCCCTACAATGCCCTTGAAACCCGGGGCTCAGAGCGCCGATTGTGGCGATTC
  AAGCAGCTCCTCATCCGACAGTGGCAATAGTGATACAGAGCAAAGTGAGCGGGAGGAGGCCAGAGCCGAG
  GCCCCTCGATTGAGAGCACCAAAATCTCGGCGCACGAGTAGGCCTAATCGGGGCCAAACCCCCTGTCCGT
  CAAACGCCGCAGAGCCAGAGCAGCCCTGGATCGCCGCTGTGCACCAGGAAAGTGACGAGCGCCCAATTTT
  CCCTCATCCGAGCAAGCCTACATTCCTGCCTCCCGTTAAACGCAAGAAGGGATTGAGAGATTCTAGGGAG
  GGCATGTTTCTGCCAAAACCAGAGGCAGGCAGTGCAATCTCCGACGTGTTTGAGGGGCGGGAAGTTTGTC
  AGCCTAAGCGGATACGACCATTCCATCCCCCTGGAAGCCCTTGGGCCAATAGACCCCTGCCCGCCTCTTT
  GGCCCCCACACCCACCGGCCCTGTCCATGAGCCTGTCGGAAGTTTGACACCGGCTCCCGTACCACAGCCT
  CTTGATCCCGCCCCTGCAGTGACCCCCGAGGCAAGCCATCTCCTGGAAGATCCGGACGAGGAAACTTCAC
  AAGCGGTCAAGGCTCTGCGGGAGATGGCAGATACCGTGATCCCCCAAAAAGAGGAAGCCGCAATTTGTGG
  ACAAATGGATCTGAGCCATCCCCCGCCCAGAGGGCACCTTGACGAGCTGACCACGACACTGGAAAGCATG
  ACTGAAGACCTGAATCTCGACTCCCCCTTGACCCCTGAGCTCAACGAGATACTCGATACATTCCTTAATG
  ATGAGTGCCTCTTGCATGCTATGCACATAAGCACCGGCTTGTCTATCTTTGACACAAGTCTGTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101134 representing YP_401674
  Red=Cloning sites Green=Tags
  
  MRPKKDGLEDFLRLTPEIKKQLGSLVSDYCNVLNKEFTAGSVEITLRSYKICKAFINEAKAHGREWGGLM
  ATLNICNFWAILRNNRVRRRAENAGNDACSIACPIVMRYVLDHLIVVTDRFFIQAPSNRVMIPATIGTAM
  YKLLKHSRVRAYTYSKVLGVDRAAIMASGKQVVEHLNRMEKEGLLSSKFKAFCKWVFTYPVLEEMFQTMV
  SSKTGHLTDDVKDVRALIKTLPRASYSSHAGQRSYVSGVLPACLLSTKSKAVETPILVSGADRMDEELMG
  NDGGASHTEARYSESGQFHAFTDELESLPSPTMPLKPGAQSADCGDSSSSSSDSGNSDTEQSEREEARAE
  APRLRAPKSRRTSRPNRGQTPCPSNAAEPEQPWIAAVHQESDERPIFPHPSKPTFLPPVKRKKGLRDSRE
  GMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWANRPLPASLAPTPTGPVHEPVGSLTPAPVPQP
  LDPAPAVTPEASHLLEDPDEETSQAVKALREMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESM
  TEDLNLDSPLTPELNEILDTFLNDECLLHAMHISTGLSIFDTSLF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007605
• ORF Size: 1815 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_007605.1, YP_401674
• RefSeq ORF: 1815
• Locus ID: 3783727
• MW: 66.6 kDa