LMP-2A (NC_007605) Virus Tagged ORF Clone

CAT#: VC101093

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for K15 [Human herpesvirus 4], codon optimized for human cell expression, YP_401631


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Reconstitution Protocol

USD 640.00

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Size
    • 10 ug

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Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Symbol LMP-2A
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101093 represents NCBI reference of YP_401631 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGTTCACTGGAGATGGTTCCTATGGGCGCTGGTCCCCCCAGCCCGGGCGGAGACCCGGATGGCTATG
ACGGCGGCAATAACTCCCAGTATCCCTCCGCATCCGGCAGTAGCGGAAACACACCAACCCCCCCAAATGA
CGAAGAGAGGGAGTCCAATGAAGAGCCCCCCCCTCCTTACGAAGATCCCTACTGGGGCAACGGCGATAGA
CACTCTGATTACCAACCTCTTGGCACTCAGGATCAGAGCCTGTACTTGGGACTGCAGCACGATGGTAACG
ATGGGTTGCCCCCACCTCCGTACAGCCCCAGGGACGATAGTAGTCAGCATATTTATGAAGAAGCTGGTCG
CGGGTCTATGAACCCTGTTTGTCTTCCTGTGATAGTAGCACCTTACCTTTTCTGGCTGGCTGCTATCGCA
GCCTCCTGTTTCACAGCCTCTGTGAGCACAGTCGTTACTGCCACTGGTCTTGCACTGAGTCTCTTGCTGC
TGGCTGCTGTTGCGAGTAGCTATGCCGCGGCTCAGAGAAAGCTGCTTACCCCTGTGACGGTGTTGACCGC
CGTAGTCACATTCTTCGCCATCTGCCTCACCTGGCGCATTGAGGATCCGCCCTTCAATTCTTTGCTCTTT
GCACTTCTCGCTGCAGCAGGTGGGCTGCAAGGAATTTATGTGCTTGTGATGCTCGTTCTGTTGATACTGG
CATACAGGCGGCGATGGAGGAGGCTCACTGTGTGTGGTGGCATTATGTTTCTGGCATGCGTTCTTGTTCT
GATCGTCGATGCCGTGCTGCAGCTGTCACCACTCCTGGGTGCCGTCACCGTGGTCTCCATGACCCTCCTT
CTTCTGGCATTTGTATTGTGGCTCTCATCCCCTGGAGGATTGGGAACTCTGGGCGCCGCATTGCTGACCC
TGGCGGCCGCACTGGCCCTGCTCGCTTCTCTGATCCTTGGTACTCTGAACTTGACCACTATGTTTCTCCT
TATGCTCCTGTGGACCCTGGTCGTACTCCTGATCTGTAGTAGTTGTAGTTCTTGTCCCCTGTCTAAAATC
CTCCTCGCACGGCTGTTCCTGTACGCCCTGGCACTGCTCCTGCTCGCCTCTGCACTGATCGCGGGTGGCA
GTATCCTGCAAACAAATTTCAAGAGCCTCTCCAGTACTGAGTTCATCCCAAACCTCTTTTGCATGCTTCT
GCTCATCGTGGCAGGTATACTGTTCATCCTGGCAATACTTACAGAGTGGGGCAGTGGCAACCGCACTTAC
GGCCCTGTATTTATGTGTCTCGGAGGGCTGCTTACTATGGTGGCCGGTGCAGTTTGGTTGACAGTTATGT
CAAATACCCTGCTGAGTGCCTGGATTCTGACGGCGGGTTTTCTCATTTTTCTGATTGGCTTCGCCCTTTT
CGGCGTGATCAGATGCTGTAGGTACTGCTGCTATTATTGTTTGACCCTCGAATCCGAGGAGAGGCCTCCA
ACGCCTTACAGGAACACAGTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101093 representing YP_401631
Red=Cloning sites Green=Tags

MGSLEMVPMGAGPPSPGGDPDGYDGGNNSQYPSASGSSGNTPTPPNDEERESNEEPPPPYEDPYWGNGDR
HSDYQPLGTQDQSLYLGLQHDGNDGLPPPPYSPRDDSSQHIYEEAGRGSMNPVCLPVIVAPYLFWLAAIA
ASCFTASVSTVVTATGLALSLLLLAAVASSYAAAQRKLLTPVTVLTAVVTFFAICLTWRIEDPPFNSLLF
ALLAAAGGLQGIYVLVMLVLLILAYRRRWRRLTVCGGIMFLACVLVLIVDAVLQLSPLLGAVTVVSMTLL
LLAFVLWLSSPGGLGTLGAALLTLAAALALLASLILGTLNLTTMFLLMLLWTLVVLLICSSCSSCPLSKI
LLARLFLYALALLLLASALIAGGSILQTNFKSLSSTEFIPNLFCMLLLIVAGILFILAILTEWGSGNRTY
GPVFMCLGGLLTMVAGAVWLTVMSNTLLSAWILTAGFLIFLIGFALFGVIRCCRYCCYYCLTLESEERPP
TPYRNTV

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_007605
ORF Size 1491 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Reference Data
RefSeq NC_007605.1, YP_401631
RefSeq ORF 1491
Locus ID 3783751
MW 53.0 kDa

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