BKRF3 (NC_007605) Virus Tagged ORF Clone

CAT#: VC101139

Myc-DDK-tagged ORF clone of viral ORF for BKRF3 [Human herpesvirus 4], codon optimized for human cell expression, YP_401679

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BKRF3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101139 represents NCBI reference of YP_401679 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCCCGGGGCCTGGACCTGTGGCTGGACGAGCATGTGTGGAAGCGCAAGCAGGAAATAGGTGTGA
  AAGGAGAAAACCTTCTGCTCCCTGACCTTTGGCTGGACTTCTTGCAGCTGAGCCCTATCTTCCAGAGAAA
  ACTCGCAGCCGTGATAGCGTGTGTGCGCAGGCTTAGAACCCAGGCCACTGTGTACCCTGAAGAAGATATG
  TGTATGGCCTGGGCACGATTCTGCGACCCCTCAGACATCAAGGTGGTTATTCTGGGCCAGGATCCATACC
  ACGGGGGACAGGCTAATGGGCTGGCCTTCTCAGTAGCCTATGGGTTCCCAGTGCCACCCTCACTTCGAAA
  CATCTATGCCGAGCTTCACCGGTCATTGCCAGAGTTCTCCCCCCCTGACCATGGCTGCCTGGACGCCTGG
  GCGTCACAAGGGGTGCTGCTTCTCAATACTATACTCACTGTGCAGAAGGGAAAACCAGGCAGCCACGCAG
  ACATCGGCTGGGCTTGGTTTACAGACCATGTCATTAGCCTTCTGTCTGAGAGGCTGAAAGCTTGCGTTTT
  CATGCTCTGGGGAGCAAAAGCCGGCGATAAGGCATCTCTGATCAACTCCAAAAAGCACCTTGTTCTTACA
  TCCCAGCACCCCAGCCCTTTGGCCCAAAACTCTACGAGAAAGTCCGCCCAGCAGAAGTTTTTGGGAAACA
  ACCACTTTGTTTTGGCGAATAACTTTCTGCGAGAAAAGGGGCTGGGAGAGATCGACTGGAGACTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101139 representing YP_401679
  Red=Cloning sites Green=Tags
  
  MASRGLDLWLDEHVWKRKQEIGVKGENLLLPDLWLDFLQLSPIFQRKLAAVIACVRRLRTQATVYPEEDM
  CMAWARFCDPSDIKVVILGQDPYHGGQANGLAFSVAYGFPVPPSLRNIYAELHRSLPEFSPPDHGCLDAW
  ASQGVLLLNTILTVQKGKPGSHADIGWAWFTDHVISLLSERLKACVFMLWGAKAGDKASLINSKKHLVLT
  SQHPSPLAQNSTRKSAQQKFLGNNHFVLANNFLREKGLGEIDWRL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007605
• ORF Size: 765 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_007605.1, YP_401679
• RefSeq ORF: 765
• Locus ID: 3783711
• MW: 28.6 kDa