BXLF2 (NC_007605) Virus Tagged ORF Clone

CAT#: VC101160

Myc-DDK-tagged ORF clone of viral ORF for BXLF2 [Human herpesvirus 4], codon optimized for human cell expression, YP_401700

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BXLF2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101160 represents NCBI reference of YP_401700 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCTTCTGTGCGTGTTTTGCCTGGTCCTGTTGTGGGAAGTGGGAGCGGCTAGCTTGAGTGAGGTGA
  AGCTTCACCTTGACATTGAGGGCCATGCCTCCCATTACACTATCCCCTGGACCGAGCTCATGGCTAAGGT
  GCCCGGGCTTTCTCCTGAGGCCCTGTGGCGCGAAGCAAACGTGACTGAGGATTTGGCGTCCATGCTCAAT
  CGCTATAAGCTGATCTACAAAACCAGCGGAACGCTGGGAATAGCCCTCGCCGAACCGGTGGACATCCCTG
  CAGTAAGTGAGGGCTCCATGCAGGTTGACGCCTCAAAAGTGCACCCAGGCGTGATCTCTGGGCTGAACTC
  TCCCGCCTGCATGCTGAGCGCACCGTTGGAGAAGCAGCTCTTTTATTATATTGGAACAATGCTGCCCAAC
  ACCCGGCCCCACTCATACGTCTTCTACCAGCTGAGATGCCATTTGAGCTACGTCGCTTTGAGCATCAACG
  GGGACAAATTCCAGTACACTGGCGCCATGACCTCCAAGTTTCTGATGGGAACCTACAAAAGGGTGACTGA
  AAAAGGAGATGAGCACGTGCTCAGCCTGGTCTTCGGCAAGACCAAAGACTTGCCAGATCTGCGAGGCCCC
  TTCAGTTACCCAAGTCTTACCTCCGCACAAAGTGGCGACTATTCATTGGTTATCGTAACTACCTTCGTTC
  ACTACGCTAATTTCCATAATTACTTTGTCCCTAATCTCAAAGACATGTTTTCACGGGCTGTGACGATGAC
  CGCGGCAAGCTATGCACGGTATGTACTGCAGAAACTCGTCCTGCTGGAGATGAAGGGCGGCTGCCGCGAA
  CCTGAATTGGACACAGAGACCTTGACAACCATGTTCGAAGTGTCTGTCGCCTTCTTTAAGGTGGGCCACG
  CGGTTGGCGAGACTGGCAATGGTTGTGTGGATTTGAGATGGCTTGCAAAGTCTTTCTTCGAACTGACCGT
  GCTGAAAGATATCATAGGCATTTGCTACGGTGCAACGGTCAAGGGCATGCAGTCCTACGGCCTGGAGAGA
  CTGGCGGCTATGCTGATGGCAACCGTGAAGATGGAGGAGTTGGGACATCTGACAACAGAAAAACAGGAGT
  ACGCCTTGAGGTTGGCTACCGTTGGATACCCCAAGGCCGGAGTCTACAGCGGACTGATTGGGGGCGCCAC
  TTCAGTCCTTCTGAGTGCTTACAACCGCCATCCTCTCTTCCAGCCACTCCACACAGTGATGCGCGAAACC
  CTTTTCATCGGTAGCCACGTGGTCCTGCGCGAACTTCGCCTTAACGTCACCACTCAGGGGCCTAACCTGG
  CTCTCTACCAGCTTCTGTCAACGGCACTCTGCTCAGCCCTCGAAATCGGGGAGGTTCTCCGGGGGTTGGC
  CCTGGGCACTGAGTCCGGGCTGTTCTCCCCTTGTTACTTGTCTCTGCGATTTGACCTGACCAGAGATAAA
  TTGCTTAGTATGGCACCCCAGGAGGCCACCCTTGATCAAGCTGCAGTGTCCAACGCAGTCGACGGGTTCC
  TGGGGCGGCTGAGCTTGGAAAGAGAGGACCGCGACGCTTGGCATCTTCCAGCTTATAAGTGTGTGGACAG
  ACTGGACAAAGTGTTGATGATCATCCCCCTGATCAACGTGACCTTTATCATCAGTAGTGATCGCGAGGTA
  CGGGGTAGCGCGCTCTATGAAGCAAGCACCACCTACCTCTCCTCATCCTTGTTTCTGTCTCCCGTCATTA
  TGAATAAGTGCTCCCAAGGTGCAGTCGCAGGAGAACCCCGGCAAATTCCAAAAATCCAGAATTTTACCAG
  GACTCAAAAAAGTTGTATATTTTGTGGATTCGCCTTGCTCAGCTACGATGAAAAGGAGGGATTGGAAACC
  ACCACCTATATTACGAGTCAGGAAGTACAGAACTCCATTCTGTCTAGTAACTACTTCGACTTCGATAACC
  TGCACGTGCATTACCTTCTTCTGACCACGAACGGCACTGTTATGGAAATTGCCGGTCTTTACGAGGAACG
  AGCTCACGTCGTCTTGGCTATAATCCTGTATTTTATTGCATTTGCTCTCGGCATTTTCCTGGTCCATAAA
  ATCGTCATGTTCTTTCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101160 representing YP_401700
  Red=Cloning sites Green=Tags
  
  MQLLCVFCLVLLWEVGAASLSEVKLHLDIEGHASHYTIPWTELMAKVPGLSPEALWREANVTEDLASMLN
  RYKLIYKTSGTLGIALAEPVDIPAVSEGSMQVDASKVHPGVISGLNSPACMLSAPLEKQLFYYIGTMLPN
  TRPHSYVFYQLRCHLSYVALSINGDKFQYTGAMTSKFLMGTYKRVTEKGDEHVLSLVFGKTKDLPDLRGP
  FSYPSLTSAQSGDYSLVIVTTFVHYANFHNYFVPNLKDMFSRAVTMTAASYARYVLQKLVLLEMKGGCRE
  PELDTETLTTMFEVSVAFFKVGHAVGETGNGCVDLRWLAKSFFELTVLKDIIGICYGATVKGMQSYGLER
  LAAMLMATVKMEELGHLTTEKQEYALRLATVGYPKAGVYSGLIGGATSVLLSAYNRHPLFQPLHTVMRET
  LFIGSHVVLRELRLNVTTQGPNLALYQLLSTALCSALEIGEVLRGLALGTESGLFSPCYLSLRFDLTRDK
  LLSMAPQEATLDQAAVSNAVDGFLGRLSLEREDRDAWHLPAYKCVDRLDKVLMIIPLINVTFIISSDREV
  RGSALYEASTTYLSSSLFLSPVIMNKCSQGAVAGEPRQIPKIQNFTRTQKSCIFCGFALLSYDEKEGLET
  TTYITSQEVQNSILSSNYFDFDNLHVHYLLLTTNGTVMEIAGLYEERAHVVLAIILYFIAFALGIFLVHK
  IVMFFL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007605
• ORF Size: 2118 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_007605.1, YP_401700
• RefSeq ORF: 2118
• Locus ID: 3783742
• MW: 78.3 kDa