BRRF1 (NC_007605) Virus Tagged ORF Clone

CAT#: VC101135

Myc-DDK-tagged ORF clone of viral ORF for BRRF1 [Human herpesvirus 4], codon optimized for human cell expression, YP_401675

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BRRF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101135 represents NCBI reference of YP_401675 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGCTCCAACAGGGGCAACGCCCGCCCATTGAAATCATTCCTGCACGAATTGTACCTGAAACATT
  ATCCAGAGGTCGGCGACGTCGTCCACTTGCTCAACACCATTGGCGTCGACTGTGACCTCCCCCCTTCACA
  TCCCCTCTTGACAGCTCAGCGCGGGCTGTTCCTGGCTCGGGTGCTGCAGGCAGTCCAGCAGCACAAACTG
  CTGGAAGATACCATAGTGCCCAAGATTCTGAAGAAGCTGGCTTATTTCCTCGAGCTTCTGAGCTACTATT
  CCCCCAAGGATGAACAGAGAGACATCGCAGAAGTCCTCGACCACTTGAAGACTAATAGAGACCTCGGGCT
  GGACGATCGCCTCTGGGCACTCATACGAAAGTTGAGACAGGACCGACACCATGCCTCAGTGAACGTGCTG
  ATGCCCGGTTCAGACTACACTGCTGTTAGCCTGCAGTACTATGATGGAATCAGCATTGGAATGCGCAAAG
  TTATCGCCGACGTGTGCAGAAGTGGTTATGCGAGTATGCCTAGCATGACAGCTACACATAACTTGAGCCA
  CCAGTTGCTTATGGCCTCAGGACCCTCCGAGGAGCCTTGCGCGTGGCGAGGCTTCTTCAATCAGGTGCTG
  CTGTGGACCGTGGCCCTGTGTAAGTTCCGGAGATGCATTTACTATAATTATATTCAAGGCTCTATAGCAA
  CCATCTCTCAGCTTCTGCACCTTGAGATCAAAGCCCTTTGTAGCTGGATCATCTCACAGGATGGTATGCG
  ACTGTTCCAACATTCCAGGCCCTTGCTTACCTTGTGGGAGAGTGTAGCCGCTAATCAGGAAGTAACAGAC
  GCGATTACATTGCCCGATTGCGCCGAATACATCGATCTCCTGAAGCACACCAAACACGTCCTCGAAAATT
  GTTCAGCGATGCAGTACAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101135 representing YP_401675
  Red=Cloning sites Green=Tags
  
  MASSNRGNARPLKSFLHELYLKHYPEVGDVVHLLNTIGVDCDLPPSHPLLTAQRGLFLARVLQAVQQHKL
  LEDTIVPKILKKLAYFLELLSYYSPKDEQRDIAEVLDHLKTNRDLGLDDRLWALIRKLRQDRHHASVNVL
  MPGSDYTAVSLQYYDGISIGMRKVIADVCRSGYASMPSMTATHNLSHQLLMASGPSEEPCAWRGFFNQVL
  LWTVALCKFRRCIYYNYIQGSIATISQLLHLEIKALCSWIISQDGMRLFQHSRPLLTLWESVAANQEVTD
  AITLPDCAEYIDLLKHTKHVLENCSAMQYK
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007605
• ORF Size: 930 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_007605.1, YP_401675
• RefSeq ORF: 930
• Locus ID: 3783728
• MW: 35.3 kDa