BFLF2 (NC_007605) Virus Tagged ORF Clone

CAT#: VC101109

Myc-DDK-tagged ORF clone of viral ORF for BFLF2 [Human herpesvirus 4], codon optimized for human cell expression, YP_401647

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BFLF2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101109 represents NCBI reference of YP_401647 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCCGTGACCCCCGACGCAGTGAACGCTCGGCAGCAGCGGCCCGCCGACCCCGCCCTGCGGCGGC
  TGATGCATCCTCACCACCGGAACTACACCGCCAGCAAGGCTAGCGCACACAGCGTCAAAAGCGTGAGCCG
  GTGTGGCAAGTCCCGGTCCGAGCTGGGCCGGATGGAGCGGGTGGGCTCCGTGGCCCGGAGCATCTGTAGC
  CGGCACACCCGGCACGGGGTCGATCGGAGCCACTTCAGCCTGCGGGACTTTTTCCGGGGCATCAGCGCCA
  ACTTCGAGCTCGGCAAGGACTTCCTCCGGGAGATGAACACCCCCATCCACGTGAGCGAGGCTGTGTTCCT
  GCCCCTGAGCCTGTGCACCCTGAGCCCCGGGCGGTGCCTGAGACTGTCCCCCTTCGGCCACAGCCTGACC
  CTGGGCTCCCACTGCGAGATTTGCATCAACCGGAGCCAGGTGCATGTGCCCCAGGAGTTCAGCAGCACCC
  AGCTGAGCTTCTTCAACAACGTGCATAAGATCATCCCCAACAAGACCTTCTACGTGTCCCTGCTGAGCAG
  CTCCCCCAGCGCCGTGAAGGCAGGCCTCAGCCAGCCAAGCCTGCTGTACGCATACCTGGTGACCGGCCAT
  TTCTGCGGCACCATCTGCCCCATTTTCAGCACCAACGGCAAAGGCCGGCTGATCATGCATCTGCTCCTGC
  AGGGGACCAGCCTGCACATCCCCGAGACCTGTCTGAAGCTGCTCTGCGAAAACATCGGCCCCACCTACGA
  ACTCGCCGTGGACCTCGTCGGCGACGCCTTTTGCATCAAGGTGAGCCCACGGGACACCGTCTACGAGAAG
  GCCGTGAACGTGGACGAGGACGCCATCTACGAGGCCATCAAGGACCTGGAGTGCGGAGACGAGCTGCGGC
  TGCAGATCATCAACTACACCCAGCTCATTCTCGAAAACAAACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101109 representing YP_401647
  Red=Cloning sites Green=Tags
  
  MAPVTPDAVNARQQRPADPALRRLMHPHHRNYTASKASAHSVKSVSRCGKSRSELGRMERVGSVARSICS
  RHTRHGVDRSHFSLRDFFRGISANFELGKDFLREMNTPIHVSEAVFLPLSLCTLSPGRCLRLSPFGHSLT
  LGSHCEICINRSQVHVPQEFSSTQLSFFNNVHKIIPNKTFYVSLLSSSPSAVKAGLSQPSLLYAYLVTGH
  FCGTICPIFSTNGKGRLIMHLLLQGTSLHIPETCLKLLCENIGPTYELAVDLVGDAFCIKVSPRDTVYEK
  AVNVDEDAIYEAIKDLECGDELRLQIINYTQLILENKQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007605
• ORF Size: 954 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_007605.1, YP_401647
• RefSeq ORF: 954
• Locus ID: 3783698
• MW: 35.4 kDa