BSRF1 (NC_007605) Virus Tagged ORF Clone

CAT#: VC101123

Myc-DDK-tagged ORF clone of viral ORF for BSRF1 [Human herpesvirus 4], codon optimized for human cell expression, YP_401663

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BSRF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101123 represents NCBI reference of YP_401663 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTTCTATCTGCCAGATTGGTCATGTTGCGGGCTGTGGCTCTTCGGTCGGCCACGCAACAGGTACA
  GCCAGCTGCCCGAGGAACCCGAGACCTTTGAGTGCCCTGATAGATGGAGGGCCGAGATCGACCTTGGCCT
  CCCGCCTGGCGTGCAGGTTGGAGACCTGCTCAGGAACGAACAGACCATGGGGTCACTGCGACAGGTCTAT
  CTGCTGGCGGTCCAGGCCAATAGTATCACTGACCATTTGAAAAGGTTTGATGCCGTCCGCGTGCCTGAAA
  GTTGTCGGGGTGTCGTGGAAGCCCAGGTAGCAAAATTGGAGGCAGTAAGAAGCGTCATATGGAATACAAT
  GATCTCCCTTGCAGTATCTGGGATCGAGATGGATGAGAATGGGCTTAAGGCACTGCTGGATAAGCAGGCG
  GGAGACTCTCTCGCACTGATGGAAATGGAGAAAGTGGCCACCGCCCTTAAGATGGACGAGACAGGCGCGT
  GGGCACAGGAGATTTCAGCAGTGGTAAGCTCAGTGACCGCACCTTCAGCCAGCGCACCTTTTATCAATTC
  TGCATTTGAGCCCGAAGTTCCAACTCCGGTCTTGGCCCCCCCTCCCGTCGTTAGACAGCCCGAGCATTCC
  GGTCCTACTGAGCTGGCTCTCACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101123 representing YP_401663
  Red=Cloning sites Green=Tags
  
  MAFYLPDWSCCGLWLFGRPRNRYSQLPEEPETFECPDRWRAEIDLGLPPGVQVGDLLRNEQTMGSLRQVY
  LLAVQANSITDHLKRFDAVRVPESCRGVVEAQVAKLEAVRSVIWNTMISLAVSGIEMDENGLKALLDKQA
  GDSLALMEMEKVATALKMDETGAWAQEISAVVSSVTAPSASAPFINSAFEPEVPTPVLAPPPVVRQPEHS
  GPTELALT
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007605
• ORF Size: 654 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_007605.1, YP_401663
• RefSeq ORF: 654
• Locus ID: 3783731
• MW: 23.9 kDa