BTRF1 (NC_007605) Virus Tagged ORF Clone

CAT#: VC101159

Myc-DDK-tagged ORF clone of viral ORF for BTRF1 [Human herpesvirus 4], codon optimized for human cell expression, YP_401699

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BTRF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101159 represents NCBI reference of YP_401699 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCAAATGCAAGCAACCCGGCGCTAGGTTTATACACGGAGCTGTTCACCTGCCTTCCGGACAGATAG
  TTTTCCACACGATTCACAGCCCGACCCTCGCTAGCGCACTCGGACTCCCTGGTGAGAATGTCCCCATCCC
  CGCTCTGTTTAGGGCATCCGGCCTGAACGTGCGAGAATCACTCCCCATGACCAATATGCGGGCGCCCATT
  ATTTCACTGGCCAGATTGATCTTGGCCCCCAACCCTTATATACTGGAGGGACAGCTCACAGTGGGCATGA
  CGCAGGACAATGGTATCCCCGTACTGTTCGCCAGACCAGTGATTGAGGTAAAAAGCGGACCAGAGTCCAA
  TATCAAAGCCTCTTCACAACTTATGATCGCCGAGGACTCTTGCCTGAACCAGATTGCACCGTTCTCAGCT
  TCTGAACACCCGGCCTTTTCTATGGTCGAGTCCGTGAAACGGGTCAGGGTAGATGAAGGAGCAAACACCC
  GAAGAACCATTAGGGATATCCTGGAAATCCCAGTGACTGTCCTGAGTTCTCTCCAGCTTTCACCCACTAA
  GTCCATTCTGAAAAAAGCCCCTGAGCCTCCTCCCCCCGAACCGCAGGCTACCTTTGATGCTACACCATAT
  GCTAGGATCTTTTATGACATCGGCCGACAAGTCCCCAAGCTCGGGAATGCGCCCGCGGCCCAGGTTTCCA
  ACGTGCTGATCGCGAATCGCTCTCACAATTCTCTGCGCTTGGTGCCAAACCCAGACCTGCTTCCTCTTCA
  ACACCTGTACCTCAAGCACGTAGTGTTGAAATCCCTCAACTTGGAAAACATAGTACAAGATTTTGAGGCC
  ATCTTCACATCCCCCTCAGACACTATTAGCGAGGCCGAAACTAAAGCATTCGAGAAGTTGGTGGAGCAAG
  CTAAGAATACAGTTGAGAATATTGTCTTTTGCCTGAACTCAATCTGCTCAACATCCACTCTTCCAGACGT
  GGTACCCGATGTGAATAATCCAAACATTTCTCTCGCACTGGAGAAGTACTTCCTGATGTTTCCTCCATCT
  GGAACCATCATGAGAAACGTCAGGTTCGCCACCCCCATAGTGCGGCTGCTGTGTCAGGGAGCCGAACTCG
  GGACAATGGCCCAATTTCTCGGCAAATACATCAAGGTCAAAAAAGAGACCGGCATGTACACCCTGGTCAA
  ATTGTATTACCTTCTGCGGATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101159 representing YP_401699
  Red=Cloning sites Green=Tags
  
  MLKCKQPGARFIHGAVHLPSGQIVFHTIHSPTLASALGLPGENVPIPALFRASGLNVRESLPMTNMRAPI
  ISLARLILAPNPYILEGQLTVGMTQDNGIPVLFARPVIEVKSGPESNIKASSQLMIAEDSCLNQIAPFSA
  SEHPAFSMVESVKRVRVDEGANTRRTIRDILEIPVTVLSSLQLSPTKSILKKAPEPPPPEPQATFDATPY
  ARIFYDIGRQVPKLGNAPAAQVSNVLIANRSHNSLRLVPNPDLLPLQHLYLKHVVLKSLNLENIVQDFEA
  IFTSPSDTISEAETKAFEKLVEQAKNTVENIVFCLNSICSTSTLPDVVPDVNNPNISLALEKYFLMFPPS
  GTIMRNVRFATPIVRLLCQGAELGTMAQFLGKYIKVKKETGMYTLVKLYYLLRI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007605
• ORF Size: 1212 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_007605.1, YP_401699
• RefSeq ORF: 1212
• Locus ID: 3783770
• MW: 44.3 kDa