LF1 (NC_007605) Virus Tagged ORF Clone

CAT#: VC101169

Myc-DDK-tagged ORF clone of viral ORF for LF1 [Human herpesvirus 4], codon optimized for human cell expression, YP_401709

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101169 represents NCBI reference of YP_401709 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCTTCAGACCGACACACAGGCCTGGAGAGTAGAAATCGGGACAAGAGGACTCATGTTTAGCAATT
  GCGTGCCCCTGCACCTCCCAGAAGGGCAATACCACAAGCTGCGGCTGCCCGTGTCTGCTTATGAAGCTCT
  CGCCGTTGCTAGATACGGACTCGTTGGCAGCCTCTGGGAGGTGCCGGCAGTGAACTCTGCTCTTCAGTGC
  CTGGCAGCCGCTGCCCCATGCAAAGATGTAAAGATCTACCCCAGCTGTATTTTTCAGGTCCATGCACCAA
  TGTTTGTCACCATAAAAACTAGTCTTCGCTGCCTGAATCCGCACGACCTGTGTCTGTGCCTTATCTGCGT
  TGGTGCCGCTATTCTCGACATACCTCTGCTGTGCGCTCCAAGAGATGGAGCTGGTGCTAGGGCAGCCGAA
  GGCCAGGCAGCAGCTGCTCAAGGTGGTAAGCTGAGAGTCTGGGGCCGACTGTCCCCCTCTAGCCCGACCA
  GCCTGTCATTGGCCTTCCCCTATGCCGGCCCCCCTCCCGTGGCCTGGTACAGACATAGTATCAACCTTAC
  TAGAAGTGAGGGGGTCGGCATTGGAAAGGATTGCGCCCAGGACCACGCTTGTCCCGTGCCTCCACAGGGA
  CACGCCAGTTCAGCTGCTGATCAAGCTGGCGTGCCAGAACGCGGGCGAAAACGGGCACACGAAGGACCGG
  GAGCCGGGGAGGCAGCTTCTGCTGGACGGGGCGACGTGGCGCTGTCACAGTCAAGGGCACTCCTGTGGCG
  GGGACTCGGTTGGGACACTGGCAGAGGTCGACTGGCCCCTGGTCTTGCCATGTCTCGGGACGCTGCCAGC
  GGATCCGTGCATCTGGATATCCAGGTTGACCGGGCCGAGGAAGGTTGGGTGTGCGATGTGCTGCTCGAGC
  CCGGCCCACCAACTGCCCGCGAGGGCTGTAGTCTGTCAATGGATCCAGGGCTGGTAACCCTGAAAGACGC
  TTGGACGCTGTTTCCCCTGCACCCTGAGCATGATGCTGTCGTCCCTCCTAAGGAAGAAATACATGTGATG
  GCTCAAGGCCACCTTCAGGGCGGTACACCATCACTCTGGGGCTTTACATTTCAGGAGGCCGCATGTGATC
  AATGGGTGCTTCGGCCTCGGGTATGGACAGCTCACTCTCCTATTAAAATGACCGTATATAATTGTGGGCA
  TAAGCCCCTGCACATAGGCCCATCAACCAGGCTGGGGTTGGCCCTTTTTTGGCCTGCCGAGCGAAGTGAT
  AACCTGGACGCCGGCCGGATTTTCTATCAATTGACATCCGGCGAGCTGTACTGGGGACGGACTGTTGCCA
  GACCCCCTACCCTCACACTGCCAGTCGACGAACTGCGGCCTTGGCCCAAACTGACACCTGAGGAGCCTAT
  GCAACAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101169 representing YP_401709
  Red=Cloning sites Green=Tags
  
  MALQTDTQAWRVEIGTRGLMFSNCVPLHLPEGQYHKLRLPVSAYEALAVARYGLVGSLWEVPAVNSALQC
  LAAAAPCKDVKIYPSCIFQVHAPMFVTIKTSLRCLNPHDLCLCLICVGAAILDIPLLCAPRDGAGARAAE
  GQAAAAQGGKLRVWGRLSPSSPTSLSLAFPYAGPPPVAWYRHSINLTRSEGVGIGKDCAQDHACPVPPQG
  HASSAADQAGVPERGRKRAHEGPGAGEAASAGRGDVALSQSRALLWRGLGWDTGRGRLAPGLAMSRDAAS
  GSVHLDIQVDRAEEGWVCDVLLEPGPPTAREGCSLSMDPGLVTLKDAWTLFPLHPEHDAVVPPKEEIHVM
  AQGHLQGGTPSLWGFTFQEAACDQWVLRPRVWTAHSPIKMTVYNCGHKPLHIGPSTRLGLALFWPAERSD
  NLDAGRIFYQLTSGELYWGRTVARPPTLTLPVDELRPWPKLTPEEPMQH
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007605
• ORF Size: 1407 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_007605.1, YP_401709
• RefSeq ORF: 1407
• Locus ID: 3783747
• MW: 50.5 kDa