BNLF2b (NC_007605) Virus Tagged ORF Clone

CAT#: VC101180

Myc-DDK-tagged ORF clone of viral ORF for BNLF2b [Human herpesvirus 4], codon optimized for human cell expression, YP_401720

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BNLF2b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101180 represents NCBI reference of YP_401720 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCCTGGCCGGCCGCTCGCCGGTTTCTATGCTACTCTGCGCCGCTCTTTCAGAAGAATGTCAAAAC
  GCTCTAAGAATAAGGCTAAAAAAGAGCGAGTACCCGTGGAAGATCGGCCTCCGACTCCAATGCCCACCAG
  TCAAAGACTTATTCGAAGGAACGCATTGGGAGGCGGCGTACGGCCAGATGCTGAGGACTGTATCCAGAGA
  TTTCATCCGCTGGAGCCGGCTCTGGGCGTGTCTACAAAGAACTTTGACCTTCTCAGCCTGCGGTGTGAGC
  TCGGTTGGTGCGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101180 representing YP_401720
  Red=Cloning sites Green=Tags
  
  MRPGRPLAGFYATLRRSFRRMSKRSKNKAKKERVPVEDRPPTPMPTSQRLIRRNALGGGVRPDAEDCIQR
  FHPLEPALGVSTKNFDLLSLRCELGWCG
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007605
• ORF Size: 294 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_007605.1, YP_401720
• RefSeq ORF: 294
• Locus ID: 3783721
• MW: 11.1 kDa