BFRF1 (NC_007605) Virus Tagged ORF Clone

CAT#: VC101111

Myc-DDK-tagged ORF clone of viral ORF for BFRF1 [Human herpesvirus 4], codon optimized for human cell expression, YP_401649

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BFRF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101111 represents NCBI reference of YP_401649 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGCCCTGAAGAAAGACTGCTGGACGAGCTGAACAACGTGATCGTGTCCTTCCTGTGCGACAGCG
  GAAGCCTCGAAGTGGAGAGATGTAGCGGCGCCCACGTGTTCTCCCGGGGGAGCTCCCAGCCCCTGTGCAC
  CGTGAAGCTGCGGCACGGCCAGATCTACCACCTGGAGTTCGTGTACAAGTTCCTGGCATTCAAGCTCAAG
  AACTGTAACTACCCCAGCAGCCCCGTGTTCGTGATCAGCAACAACGGCCTGGCTACAACACTGCGGTGCT
  TCCTGCACGAGCCCAGCGGCCTGCGGTCCGGCCAGAGCGGCCCCTGCCTCGGCCTGAGCACCGACGTCGA
  TCTGCCCAAGAACAGCATCATCATGCTCGGCCAGGACGACTTCATCAAGTTTAAAAGCCCTCTGGTGTTT
  CCCGCCGAGCTGGACCTGCTGAAAAGCATGGTCGTGTGCAGAGCCTACATCACCGAGCACCGGACCACCA
  TGCAGTTCCTGGTGTTCCAGGCCGCTAATGCTCAGAAGGCCAGCCGGGTGATGGACATGATCAGCGATAT
  GAGCCAGCAACTGAGCCGGAGCGGGCAAGTGGAGGACACCGGCGCACGGGTGACTGGAGGCGGAGGCCCA
  CGGCCCGGCGTGACACACAGCGGCTGCCTGGGCGACAGCCACGTGAGAGGCCGGGGCGGGTGGGATCTGG
  ACAACTTTAGCGAGGCCGAGACCGAGGACGAAGCCAGCTACGCCCCATGGCGGGACAAGGACAGCTGGAG
  CGAGAGCGAAGCAGCCCCCTGGAAGAAGGAGCTGGTGCGGCATCCCATCCGGAGACACCGGACACGGGAA
  ACCCGGCGGATGCGGGGCAGCCACTCCCGGGTGGAACACGTGCCCCCCGAGACCCGGGAGACTGTGGTGG
  GCGGGGCATGGCGGTACTCCTGGCGGGCTACCCCCTACCTCGCCCGGGTGCTCGCCGTCACTGCCGTGGC
  CCTGCTCCTGATGTTCCTCAGATGGACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101111 representing YP_401649
  Red=Cloning sites Green=Tags
  
  MASPEERLLDELNNVIVSFLCDSGSLEVERCSGAHVFSRGSSQPLCTVKLRHGQIYHLEFVYKFLAFKLK
  NCNYPSSPVFVISNNGLATTLRCFLHEPSGLRSGQSGPCLGLSTDVDLPKNSIIMLGQDDFIKFKSPLVF
  PAELDLLKSMVVCRAYITEHRTTMQFLVFQAANAQKASRVMDMISDMSQQLSRSGQVEDTGARVTGGGGP
  RPGVTHSGCLGDSHVRGRGGWDLDNFSEAETEDEASYAPWRDKDSWSESEAAPWKKELVRHPIRRHRTRE
  TRRMRGSHSRVEHVPPETRETVVGGAWRYSWRATPYLARVLAVTAVALLLMFLRWT
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007605
• ORF Size: 1008 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_007605.1, YP_401649
• RefSeq ORF: 1008
• Locus ID: 3783699
• MW: 37.6 kDa