BdRF1 (NC_007605) Virus Tagged ORF Clone

CAT#: VC101165

Myc-DDK-tagged ORF clone of viral ORF for BdRF1 [Human herpesvirus 4], codon optimized for human cell expression, YP_401705

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BdRF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101165 represents NCBI reference of YP_401705 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTAGTGGCAATGCGGGCGAAGGCGCCACGGCCTGTGGGGGCAGCGCCGCTGCAGGGCAGGATCTGA
  TCTCTGTGCCCAGGAATACCTTTATGACCCTGCTGCAGACCAATCTCGACAACAAACCGCCCAGACAGAC
  GCCACTCCCCTACGCAGCCCCACTGCCGCCCTTCAGTCACCAGGCCATTGCCACTGCCCCTTCATATGGC
  CCTGGAGCTGGGGCAGTAGCCCCTGCCGGCGGCTACTTTACAAGTCCGGGCGGTTATTATGCCGGCCCCG
  CTGGAGGCGACCCTGGCGCATTCTTGGCCATGGATGCACACACCTATCATCCACATCCGCATCCACCCCC
  TGCCTACTTTGGGCTCCCAGGCTTGTTCGGACCCCCTCCCCCCGTTCCACCATATTATGGCAGCCACCTG
  CGCGCAGACTATGTGCCCGCTCCAAGTAGATCTAATAAGAGAAAAAGGGATCCTGAAGAGGATGAGGAAG
  GCGGGGGTCTGTTCCCAGGCGAGGATGCGACGCTCTATAGAAAGGACATCGCCGGTCTGTCCAAGTCAGT
  CAATGAGCTCCAGCACACTCTGCAAGCTCTGCGGCGCGAAACGCTGTCCTACGGCCACACGGGGGTGGGA
  TACTGCCCACAGCAGGGACCTTGCTACACACATTCCGGCCCTTATGGATTTCAACCCCATCAGAGTTACG
  AGGTGCCCAGATATGTCCCACACCCTCCTCCACCTCCAACCAGTCATCAGGCGGCCCAGGCCCAGCCACC
  TCCACCTGGCACTCAGGCTCCTGAAGCTCACTGCGTTGCAGAATCTACAATCCCTGAGGCTGGTGCCGCC
  GGGAATAGCGGACCACGAGAGGACACCAATCCTCAGCAACCAACAACAGAGGGGCATCACAGAGGGAAAA
  AACTTGTGCAGGCATCCGCCTCCGGAGTCGCTCAGTCTAAAGAACCTACCACGCCAAAAGCAAAAAGTGT
  GTCCGCTCATCTCAAGTCAATCTTCTGTGAAGAACTGCTCAATAAGCGCGTGGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101165 representing YP_401705
  Red=Cloning sites Green=Tags
  
  MLSGNAGEGATACGGSAAAGQDLISVPRNTFMTLLQTNLDNKPPRQTPLPYAAPLPPFSHQAIATAPSYG
  PGAGAVAPAGGYFTSPGGYYAGPAGGDPGAFLAMDAHTYHPHPHPPPAYFGLPGLFGPPPPVPPYYGSHL
  RADYVPAPSRSNKRKRDPEEDEEGGGLFPGEDATLYRKDIAGLSKSVNELQHTLQALRRETLSYGHTGVG
  YCPQQGPCYTHSGPYGFQPHQSYEVPRYVPHPPPPPTSHQAAQAQPPPPGTQAPEAHCVAESTIPEAGAA
  GNSGPREDTNPQQPTTEGHHRGKKLVQASASGVAQSKEPTTPKAKSVSAHLKSIFCEELLNKRVA
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007605
• ORF Size: 1035 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_007605.1, YP_401705
• RefSeq ORF: 1035
• Locus ID: 3783696
• MW: 36.1 kDa