BdRF1 (NC_007605) Virus Tagged ORF Clone

CAT#: VC101165

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for BdRF1 [Human herpesvirus 4], codon optimized for human cell expression, YP_401705


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Reconstitution Protocol

USD 430.00

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Size
    • 10 ug

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Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Symbol BdRF1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101165 represents NCBI reference of YP_401705 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTTAGTGGCAATGCGGGCGAAGGCGCCACGGCCTGTGGGGGCAGCGCCGCTGCAGGGCAGGATCTGA
TCTCTGTGCCCAGGAATACCTTTATGACCCTGCTGCAGACCAATCTCGACAACAAACCGCCCAGACAGAC
GCCACTCCCCTACGCAGCCCCACTGCCGCCCTTCAGTCACCAGGCCATTGCCACTGCCCCTTCATATGGC
CCTGGAGCTGGGGCAGTAGCCCCTGCCGGCGGCTACTTTACAAGTCCGGGCGGTTATTATGCCGGCCCCG
CTGGAGGCGACCCTGGCGCATTCTTGGCCATGGATGCACACACCTATCATCCACATCCGCATCCACCCCC
TGCCTACTTTGGGCTCCCAGGCTTGTTCGGACCCCCTCCCCCCGTTCCACCATATTATGGCAGCCACCTG
CGCGCAGACTATGTGCCCGCTCCAAGTAGATCTAATAAGAGAAAAAGGGATCCTGAAGAGGATGAGGAAG
GCGGGGGTCTGTTCCCAGGCGAGGATGCGACGCTCTATAGAAAGGACATCGCCGGTCTGTCCAAGTCAGT
CAATGAGCTCCAGCACACTCTGCAAGCTCTGCGGCGCGAAACGCTGTCCTACGGCCACACGGGGGTGGGA
TACTGCCCACAGCAGGGACCTTGCTACACACATTCCGGCCCTTATGGATTTCAACCCCATCAGAGTTACG
AGGTGCCCAGATATGTCCCACACCCTCCTCCACCTCCAACCAGTCATCAGGCGGCCCAGGCCCAGCCACC
TCCACCTGGCACTCAGGCTCCTGAAGCTCACTGCGTTGCAGAATCTACAATCCCTGAGGCTGGTGCCGCC
GGGAATAGCGGACCACGAGAGGACACCAATCCTCAGCAACCAACAACAGAGGGGCATCACAGAGGGAAAA
AACTTGTGCAGGCATCCGCCTCCGGAGTCGCTCAGTCTAAAGAACCTACCACGCCAAAAGCAAAAAGTGT
GTCCGCTCATCTCAAGTCAATCTTCTGTGAAGAACTGCTCAATAAGCGCGTGGCA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101165 representing YP_401705
Red=Cloning sites Green=Tags

MLSGNAGEGATACGGSAAAGQDLISVPRNTFMTLLQTNLDNKPPRQTPLPYAAPLPPFSHQAIATAPSYG
PGAGAVAPAGGYFTSPGGYYAGPAGGDPGAFLAMDAHTYHPHPHPPPAYFGLPGLFGPPPPVPPYYGSHL
RADYVPAPSRSNKRKRDPEEDEEGGGLFPGEDATLYRKDIAGLSKSVNELQHTLQALRRETLSYGHTGVG
YCPQQGPCYTHSGPYGFQPHQSYEVPRYVPHPPPPPTSHQAAQAQPPPPGTQAPEAHCVAESTIPEAGAA
GNSGPREDTNPQQPTTEGHHRGKKLVQASASGVAQSKEPTTPKAKSVSAHLKSIFCEELLNKRVA

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_007605
ORF Size 1035 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Reference Data
RefSeq NC_007605.1, YP_401705
RefSeq ORF 1035
Locus ID 3783696
MW 36.1 kDa

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