BLRF1 (NC_007605) Virus Tagged ORF Clone

CAT#: VC101125

Myc-DDK-tagged ORF clone of viral ORF for BLRF1 [Human herpesvirus 4], codon optimized for human cell expression, YP_401665

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BLRF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101125 represents NCBI reference of YP_401665 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAAAAGTCCTGCGCAAACCTTTCGCAAAGGCTGTGCCTCTGTTGTTTCTGGCTGCCACATGGCTTC
  TCACTGGAGTGCTGCCGGCCGGAGCCTCTTCTCCCACTAATGCGGCCGCCGCCTCACTTACAGAGGCACA
  GGATCAGTTCTACTCATACACATGTAACGCCGATACATTTAGCCCAAGTTTGACCTCCTTCGCTTCTATC
  TGGGCTTTGCTTACCCTGGTGCTCGTCATCATCGCCTCTGCAATCTATCTCATGTACGTCTGTTTTAACA
  AGTTCGTGAACACTCTCCTTACCGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101125 representing YP_401665
  Red=Cloning sites Green=Tags
  
  MGKVLRKPFAKAVPLLFLAATWLLTGVLPAGASSPTNAAAASLTEAQDQFYSYTCNADTFSPSLTSFASI
  WALLTLVLVIIASAIYLMYVCFNKFVNTLLTD
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007605
• ORF Size: 306 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_007605.1, YP_401665
• RefSeq ORF: 306
• Locus ID: 3783716
• MW: 10.9 kDa