BBRF3 (NC_007605) Virus Tagged ORF Clone

CAT#: VC101145

Myc-DDK-tagged ORF clone of viral ORF for BBRF3 [Human herpesvirus 4], codon optimized for human cell expression, YP_401685

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BBRF3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101145 represents NCBI reference of YP_401685 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAGTTCTAAAAACGACACCTTTGTTTACCGGACCTGGGTCAAGACGCTGGTGGTTTACTTCGTCA
  TGTTCGTTATGAGTGCCGTCGTACCCATTACTGCCATGTTCCCTAATCTGGGGTACCCGTGTTACTTTAA
  CGCGCTCGTTGACTATGGGGCTTTGAACCTGACTAATTACAACCTCGCACACCATCTCACTCCTACTCTT
  TACCTGGAACCTCCTGAGATGTTCGTGTACATTACACTCGTCTTCATTGCCGACTGTGTCGCATTTATTT
  ACTATGCATGCGGAGAGGTGGCCCTCATTAAGGCTAGGAAAAAAGTGTCTGGATTGACGGACCTTAGCGC
  TTGGGTTTCTGCCGTGGGCTCACCCACCGTTCTTTTCCTGGCTATCCTCAAACTTTGGTCCATACAGGTG
  TTCATTCAGGTGTTGTCCTATAAACATGTGTTTCTGAGTGCCTTTGTCTACTTTCTGCACTTTCTGGCCA
  GTGTCTTGCACGCCTGCGCATGTGTGACCCGGTTCTCCCCAGTGTGGGTGGTGAAGGCCCAAGACAACTC
  CATACCCCAAGATACCTTTCTGTGGTGGGTGGTATTCTATCTGAAACCAGTAGTCACTAACCTGTACCTG
  GGGTGTCTGGCACTCGAGACACTTGTCTTCTCTCTGAGTGTTTTCCTGGCACTTGGGAACAGCTTTTATT
  TTATGGTCGGCGACATGGTGCTTGGGGCTGTCAATTTGTTCCTGATATTGCCCATTTTTTGGTACATCCT
  GACAGAGGTGTGGCTTGCCAGTTTCCTGCGGCATAACTTTGGATTCTACTGTGGAATGTTCATTGCCTCT
  ATCATTCTCATTCTTCCTCTGGTTAGATATGAGGCTGTTTTCGTCTCCGCCAAACTCCATACCACAGTTG
  CCATCAACGTGGCCATTATCCCAATTTTGTGCAGCGTGGCCATGCTTATCCGGATTTGTAGGATATTCAA
  AAGCATGAGACAGGGAACCGATTACGTCCCTGTTAGCGAGACCGTGGAGCTCGAATTGGAAAGCGAACCA
  CGGCCCAGACCTAGCCGGACCCCTAGCCCGGGCCGCAACAGAAGAAGATCTTCCACCTCCTCTAGCTCTA
  GTCGCTCCACCAGACGGCAGCGGCCGGTCAGTACTCAAGCGCTGGTGAGTAGTGTCCTGCCTATGACAAC
  CGATTCAGAAGAGGAAATCTTTCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101145 representing YP_401685
  Red=Cloning sites Green=Tags
  
  MKSSKNDTFVYRTWVKTLVVYFVMFVMSAVVPITAMFPNLGYPCYFNALVDYGALNLTNYNLAHHLTPTL
  YLEPPEMFVYITLVFIADCVAFIYYACGEVALIKARKKVSGLTDLSAWVSAVGSPTVLFLAILKLWSIQV
  FIQVLSYKHVFLSAFVYFLHFLASVLHACACVTRFSPVWVVKAQDNSIPQDTFLWWVVFYLKPVVTNLYL
  GCLALETLVFSLSVFLALGNSFYFMVGDMVLGAVNLFLILPIFWYILTEVWLASFLRHNFGFYCGMFIAS
  IILILPLVRYEAVFVSAKLHTTVAINVAIIPILCSVAMLIRICRIFKSMRQGTDYVPVSETVELELESEP
  RPRPSRTPSPGRNRRRSSTSSSSSRSTRRQRPVSTQALVSSVLPMTTDSEEEIFP
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007605
• ORF Size: 1215 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_007605.1, YP_401685
• RefSeq ORF: 1215
• Locus ID: 3783687
• MW: 45.8 kDa