LF2 (NC_007605) Virus Tagged ORF Clone

CAT#: VC101168

Myc-DDK-tagged ORF clone of viral ORF for LF2 [Human herpesvirus 4], codon optimized for human cell expression, YP_401708

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LF2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101168 represents NCBI reference of YP_401708 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAGGCCTACCCCGGTGGTGCTCACGCAGCGCTCGCATCCCGCAGAAGCTCATTCCGGAACTCTT
  TGCGGAGGCTTAGACCAACCGAGAAACCTGATACGTCCTTCATGAGAGGCGTGTGGAAGTATGAAATCTT
  TCCTAGTTATGTCAGAGTTACGAACAAGCAGGTGCTCCAGCTCGACGCTCAGTGTCAAGAACTCCCACCA
  TGTCCTTCCGTGGGGCAAATCTTGAGCTTTAAGCTGCCAAGCTTCAGTTTCAATACAACCACCTACGGGT
  CCCGGTATTTTACCGTCGCATTCCTCTTTTTTGGAGCCGAGGACAATGAGGTATTCCTGAAGCCATTCTT
  CGTCATGCACTCAGACCAGGACATCGTGCTTTCCGTGCTGAACCCTCGCTCCCTGTTCATCGAAAAAGGC
  AAGTTCACGTGGTATATCGTCCCAATAAGGCTCGTGAAGAATCCCTATCTGTATCTGCAGATTCTTCCTG
  GTCAATCAGACATACAGCTCACCAGGAGTTGTACTCAGAGTGGCGATAAGCTGAATACGTCAGAACCTCA
  GATATTTCTGAGCGGGTCCCCCGTCACCTCACAGGATGAGTGTCTTCCCTATCTGCTTGCCCAACACACA
  CCCCCTTTTTTGAAATCCTACGCCCGGATTCACACCTTTCCTGGGAAGGTATGTCCCGTGAACGCAATCC
  GGAGGGGAAAGGGATACGTGCGCGTGTCTGTCGATACACCTGACCTGAAAAGAGAAGGCCCACTGAACGT
  CAAGGTTGGTATGACTTTGCTTGACGACGTCATCATAGCGTTTCGGTATAACCCCTACCCTAAGAGCCAT
  TGGAGATGGGACGGGGAATCCACAGATATCCGCTACTTCGGGTCTCCCGTAATCATACCGCCAAACTTTA
  TCACCGAGCTGGAATACAACAACACCTACGAAGCTCCGCTGTCATCCAAGATCACTGCCGTGGTTGTCTC
  CCATTCTTCAAACCCCGTGTTCTATGTCTATCCTCAGGAGTGGAAACCTGGGCAGACACTGAAACTGACC
  GTTAGGAACATTTCCAACAATCCCATCACAATTGTGACCGGACAATCTATGGCCCAGGCTTTTTTCATCT
  ACGCGGGCGACCCTAGCATTTCAACCATCATGAGACGGTACATCCAACGGCAGGGGTGTGCGCTGACACT
  GCCTGGGAATATCGTGGTGGAGAGCTCCAGTCTCCCTACCTTCGAAAGAATAAACAAGACTTTTAACGGC
  AACATTGTGGCGTCAGAAGGTACACTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101168 representing YP_401708
  Red=Cloning sites Green=Tags
  
  MAEAYPGGAHAALASRRSSFRNSLRRLRPTEKPDTSFMRGVWKYEIFPSYVRVTNKQVLQLDAQCQELPP
  CPSVGQILSFKLPSFSFNTTTYGSRYFTVAFLFFGAEDNEVFLKPFFVMHSDQDIVLSVLNPRSLFIEKG
  KFTWYIVPIRLVKNPYLYLQILPGQSDIQLTRSCTQSGDKLNTSEPQIFLSGSPVTSQDECLPYLLAQHT
  PPFLKSYARIHTFPGKVCPVNAIRRGKGYVRVSVDTPDLKREGPLNVKVGMTLLDDVIIAFRYNPYPKSH
  WRWDGESTDIRYFGSPVIIPPNFITELEYNNTYEAPLSSKITAVVVSHSSNPVFYVYPQEWKPGQTLKLT
  VRNISNNPITIVTGQSMAQAFFIYAGDPSISTIMRRYIQRQGCALTLPGNIVVESSSLPTFERINKTFNG
  NIVASEGTL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007605
• ORF Size: 1287 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_007605.1, YP_401708
• RefSeq ORF: 1287
• Locus ID: 3783748
• MW: 48.4 kDa