BVRF1 (NC_007605) Virus Tagged ORF Clone

CAT#: VC101163

Myc-DDK-tagged ORF clone of viral ORF for BVRF1 [Human herpesvirus 4], codon optimized for human cell expression, YP_401703

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BVRF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101163 represents NCBI reference of YP_401703 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCTGTCTGGTCACGTCCTCATCGACCCAGCTCGACTTCCTAGAGATACCGGCCCCGAGTTGATGT
  GGGCTCCCTCTTTGCGCAATTCTCTTAGAGTCTCACCTGAAGCTCTGGAACTCGCTGAAAGAGAAGCTGA
  GAGAGCGCGCTCCGAGCGATGGGATCGCTGCGCTCAGGTGCTCAAAAACCGCCTCCTGAGGGTGGAACTC
  GATGGCATTATGCGCGATCACCTGGCCAGGGCCGAAGAAATTCGACAGGATCTTGATGCAGTGGTTGCCT
  TCTCAGACGGCCTGGAGTCCATGCAGGTTCGATCACCATCAACTGGAGGGCGGAGCGCCCCCGCACCCCC
  ATCACCCTCTCCTGCACAGCCATTCACAAGACTGACTGGCAACGCCCAGTACGCAGTGAGTATCTCACCA
  ACAGATCCCCCTCTTATGGTGGCCGGATCACTGGCACAGACCCTGCTGGGGAACTTGTATGGTAACATAA
  ACCAATGGGTACCATCTTTCGGGCCTTGGTACAGGACCATGTCAGCTAACGCAATGCAGCGCCGCGTGTT
  CCCTAAACAGCTGCGCGGCAATCTGAACTTTACCAATTCCGTTAGTCTCAAGTTGATGACTGAAGTTGTT
  GCCGTGCTTGAGGGTACAACTCAAGACTTCTTCTCAGACGTGCGACACCTTCCCGATCTGCAGGCCGCAC
  TGATCCTGTCCGTTGCCTACCTCTTGCTGCAGGGCGGAAGTTCCCACCAGCAGCGCCCCCTCCCAGCCAG
  CAGGGAGGAGCTTCTGGAACTCGGACCAGAGTCACTGGAAAAAATAATTGCTGACCTGAAAGCAAAGTCT
  CCAGGCGGGAATTTCATGATCCTGACCAGCGGAAATAAGGAGGCAAGACAAAGTATTGCACCACTCAACC
  GGCAAGCCGCTTACCCGCCTGGGACATTCGCTGATAATAAAATCTACAATTTGTTCGTTGGAGCTGGGCT
  GTTGCCCACAACGGCCGCCCTCAACGTGCCAGGGGCCGCGGGACGGGATAGAGACCTCGTCTACAGAATA
  GCTAATCAGATTTTTGGTGAAGACGTACCTCCGTTCTCAAGCCACCAGTGGAACCTCCGGGTTGGACTCG
  CCGCACTCGAAGCTCTGATGTTGGTGTATACCCTGTGCGAAACCGCAAATCTGGCGGAGGCTGCTACAAG
  ACGGCTCCACCTGTCAAGCCTCCTGCCCCAGGCCATGCAGCGCAGGAAGCCAGCAATGGCGAGCGCTGGC
  ATGCCTGGAGCCTATCCCGTGCAAACTCTGTTTCGGCATGGCGAGTTGTTTCGGTTCATCTGGGCTCACT
  ATGTCAGACCAACCGTGGCCGCTGATCCTCAGGCAAGCATCAGCTCTCTCTTTCCGGGCCTCGTGTTGCT
  GGCACTCGAACTGAAGTTGATGGATGGACAAGCCCCATCACATTACGCCATTAATCTGACCGGTCAGAAG
  TTCGACACCCTCTTTGAGATAATTAACCAGAAGCTGCTGTTCCACGACCCCGCAGCCATGCTGGCCGCAA
  GGACCCAGCTTCGCCTGGCCTTCGAGGACGGCGTCGGCGTGGCTTTGGGGAGGCCTTCACCTATGCTGGC
  AGCTCGGGAAATTCTGGAGCGACAGTTCTCTGCATCTGATGACTACGATCGCCTGTATTTTCTTACCTTG
  GGTTATTTGGCCAGCCCAGTGGCACCTTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101163 representing YP_401703
  Red=Cloning sites Green=Tags
  
  MALSGHVLIDPARLPRDTGPELMWAPSLRNSLRVSPEALELAEREAERARSERWDRCAQVLKNRLLRVEL
  DGIMRDHLARAEEIRQDLDAVVAFSDGLESMQVRSPSTGGRSAPAPPSPSPAQPFTRLTGNAQYAVSISP
  TDPPLMVAGSLAQTLLGNLYGNINQWVPSFGPWYRTMSANAMQRRVFPKQLRGNLNFTNSVSLKLMTEVV
  AVLEGTTQDFFSDVRHLPDLQAALILSVAYLLLQGGSSHQQRPLPASREELLELGPESLEKIIADLKAKS
  PGGNFMILTSGNKEARQSIAPLNRQAAYPPGTFADNKIYNLFVGAGLLPTTAALNVPGAAGRDRDLVYRI
  ANQIFGEDVPPFSSHQWNLRVGLAALEALMLVYTLCETANLAEAATRRLHLSSLLPQAMQRRKPAMASAG
  MPGAYPVQTLFRHGELFRFIWAHYVRPTVAADPQASISSLFPGLVLLALELKLMDGQAPSHYAINLTGQK
  FDTLFEIINQKLLFHDPAAMLAARTQLRLAFEDGVGVALGRPSPMLAAREILERQFSASDDYDRLYFLTL
  GYLASPVAPS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007605
• ORF Size: 1710 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_007605.1, YP_401703
• RefSeq ORF: 1710
• Locus ID: 3783732
• MW: 62.5 kDa