BMRF1 (NC_007605) Virus Tagged ORF Clone

CAT#: VC101119

Myc-DDK-tagged ORF clone of viral ORF for BMRF1 [Human herpesvirus 4], codon optimized for human cell expression, YP_401657

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BMRF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101119 represents NCBI reference of YP_401657 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACAACACAGACTCTCAGATTTAAGACTAAGGCCCTGGCTGTGCTGTCCAAGTGCTACGACCACG
  CTCAGACACATCTCAAGGGAGGAGTGCTGCAGGTGAACCTGCTGTCAGTAAACTATGGTGGACCCAGGCT
  CGCGGCCGTAGCTAACGCCGGAACCGCAGGACTCATCAGCTTCGAGGTGTCTCCCGATGCAGTCGCCGAG
  TGGCAGAACCACCAATCTCCTGAGGAAGCTCCTGCTGCTGTGAGCTTCAGGAATTTGGCCTATGGTAGGA
  CTTGTGTTCTGGGGAAGGAACTGTTCGGATCAGCCGTGGAGCAGGCCTCTCTGCAGTTCTACAAAAGACC
  CCAGGGGGGATCTCGCCCTGAATTTGTTAAGCTGACCATGGAGTATGATGACAAAGTGAGTAAATCCCAC
  CATACTTGCGCCCTCATGCCTTACATGCCCCCAGCCAGCGATCGATTGAGAAACGAACAGATGATTGGCC
  AGGTGCTCCTCATGCCAAAAACCGCCAGTTCCCTGCAGAAGTGGGCAAGACAGCAGGGGTCAGGTGGAGT
  GAAGGTAACCCTGAATCCCGATCTGTATGTGACCACCTACACTTCAGGCGAGGCTTGTCTGACACTTGAC
  TACAAACCTCTCAGTGTGGGACCCTATGAGGCATTTACGGGGCCTGTTGCCAAGGCGCAGGATGTGGGTG
  CGGTGGAAGCGCACGTAGTTTGCAGTGTGGCAGCAGATTCACTGGCCGCCGCACTGAGTCTGTGCCGGAT
  CCCCGCCGTTTCCGTACCAATTCTGAGATTCTACCGAAGCGGCATCATCGCTGTGGTTGCAGGGTTGCTG
  ACAAGCGCAGGGGATTTGCCCTTGGACCTGAGCGTTATCCTGTTTAATCACGCGAGTGAGGAGGCCGCCG
  CGTCCACTGCCTCTGAACCAGAGGACAAGTCTCCCCGCGTGCAACCTCTGGGAACCGGCCTTCAACAGAG
  ACCGCGGCATACTGTATCACCCTCTCCTAGCCCGCCCCCACCTCCCCGAACACCAACCTGGGAATCACCT
  GCCAGACCTGAGACACCTTCTCCTGCCATCCCCAGCCACAGTAGCAACACTGCTCTGGAAAGGCCTCTCG
  CCGTGCAGCTGGCTAGAAAACGAACCAGTAGCGAAGCCCGGCAGAAACAGAAACACCCCAAAAAGGTGAA
  GCAAGCATTCAATCCTCTTATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101119 representing YP_401657
  Red=Cloning sites Green=Tags
  
  METTQTLRFKTKALAVLSKCYDHAQTHLKGGVLQVNLLSVNYGGPRLAAVANAGTAGLISFEVSPDAVAE
  WQNHQSPEEAPAAVSFRNLAYGRTCVLGKELFGSAVEQASLQFYKRPQGGSRPEFVKLTMEYDDKVSKSH
  HTCALMPYMPPASDRLRNEQMIGQVLLMPKTASSLQKWARQQGSGGVKVTLNPDLYVTTYTSGEACLTLD
  YKPLSVGPYEAFTGPVAKAQDVGAVEAHVVCSVAADSLAAALSLCRIPAVSVPILRFYRSGIIAVVAGLL
  TSAGDLPLDLSVILFNHASEEAAASTASEPEDKSPRVQPLGTGLQQRPRHTVSPSPSPPPPPRTPTWESP
  ARPETPSPAIPSHSSNTALERPLAVQLARKRTSSEARQKQKHPKKVKQAFNPLI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007605
• ORF Size: 1212 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_007605.1, YP_401657
• RefSeq ORF: 1212
• Locus ID: 3783718
• MW: 43.4 kDa