BMRF1 (NC_007605) Virus Tagged ORF Clone

CAT#: VC101119

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for BMRF1 [Human herpesvirus 4], codon optimized for human cell expression, YP_401657


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Reconstitution Protocol

USD 500.00

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Size
    • 10 ug

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Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Symbol BMRF1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101119 represents NCBI reference of YP_401657 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGACAACACAGACTCTCAGATTTAAGACTAAGGCCCTGGCTGTGCTGTCCAAGTGCTACGACCACG
CTCAGACACATCTCAAGGGAGGAGTGCTGCAGGTGAACCTGCTGTCAGTAAACTATGGTGGACCCAGGCT
CGCGGCCGTAGCTAACGCCGGAACCGCAGGACTCATCAGCTTCGAGGTGTCTCCCGATGCAGTCGCCGAG
TGGCAGAACCACCAATCTCCTGAGGAAGCTCCTGCTGCTGTGAGCTTCAGGAATTTGGCCTATGGTAGGA
CTTGTGTTCTGGGGAAGGAACTGTTCGGATCAGCCGTGGAGCAGGCCTCTCTGCAGTTCTACAAAAGACC
CCAGGGGGGATCTCGCCCTGAATTTGTTAAGCTGACCATGGAGTATGATGACAAAGTGAGTAAATCCCAC
CATACTTGCGCCCTCATGCCTTACATGCCCCCAGCCAGCGATCGATTGAGAAACGAACAGATGATTGGCC
AGGTGCTCCTCATGCCAAAAACCGCCAGTTCCCTGCAGAAGTGGGCAAGACAGCAGGGGTCAGGTGGAGT
GAAGGTAACCCTGAATCCCGATCTGTATGTGACCACCTACACTTCAGGCGAGGCTTGTCTGACACTTGAC
TACAAACCTCTCAGTGTGGGACCCTATGAGGCATTTACGGGGCCTGTTGCCAAGGCGCAGGATGTGGGTG
CGGTGGAAGCGCACGTAGTTTGCAGTGTGGCAGCAGATTCACTGGCCGCCGCACTGAGTCTGTGCCGGAT
CCCCGCCGTTTCCGTACCAATTCTGAGATTCTACCGAAGCGGCATCATCGCTGTGGTTGCAGGGTTGCTG
ACAAGCGCAGGGGATTTGCCCTTGGACCTGAGCGTTATCCTGTTTAATCACGCGAGTGAGGAGGCCGCCG
CGTCCACTGCCTCTGAACCAGAGGACAAGTCTCCCCGCGTGCAACCTCTGGGAACCGGCCTTCAACAGAG
ACCGCGGCATACTGTATCACCCTCTCCTAGCCCGCCCCCACCTCCCCGAACACCAACCTGGGAATCACCT
GCCAGACCTGAGACACCTTCTCCTGCCATCCCCAGCCACAGTAGCAACACTGCTCTGGAAAGGCCTCTCG
CCGTGCAGCTGGCTAGAAAACGAACCAGTAGCGAAGCCCGGCAGAAACAGAAACACCCCAAAAAGGTGAA
GCAAGCATTCAATCCTCTTATA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101119 representing YP_401657
Red=Cloning sites Green=Tags

METTQTLRFKTKALAVLSKCYDHAQTHLKGGVLQVNLLSVNYGGPRLAAVANAGTAGLISFEVSPDAVAE
WQNHQSPEEAPAAVSFRNLAYGRTCVLGKELFGSAVEQASLQFYKRPQGGSRPEFVKLTMEYDDKVSKSH
HTCALMPYMPPASDRLRNEQMIGQVLLMPKTASSLQKWARQQGSGGVKVTLNPDLYVTTYTSGEACLTLD
YKPLSVGPYEAFTGPVAKAQDVGAVEAHVVCSVAADSLAAALSLCRIPAVSVPILRFYRSGIIAVVAGLL
TSAGDLPLDLSVILFNHASEEAAASTASEPEDKSPRVQPLGTGLQQRPRHTVSPSPSPPPPPRTPTWESP
ARPETPSPAIPSHSSNTALERPLAVQLARKRTSSEARQKQKHPKKVKQAFNPLI

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_007605
ORF Size 1212 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Reference Data
RefSeq NC_007605.1, YP_401657
RefSeq ORF 1212
Locus ID 3783718
MW 43.4 kDa

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