LMP-2B (NC_007605) Virus Tagged ORF Clone

CAT#: VC101094

Myc-DDK-tagged ORF clone of viral ORF for LMP-2B [Human herpesvirus 4], codon optimized for human cell expression, YP_401632

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LMP-2B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101094 represents NCBI reference of YP_401632 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCCAGTTTGTCTTCCGGTCATCGTGGCCCCGTATCTCTTCTGGCTGGCAGCCATAGCCGCCTCCT
  GCTTCACCGCTAGTGTGTCCACAGTTGTGACAGCCACCGGACTGGCTCTCTCTCTGTTGCTGCTTGCGGC
  TGTTGCCTCCTCATATGCCGCAGCACAGAGGAAACTGCTGACACCTGTGACAGTACTGACAGCAGTGGTT
  ACCTTCTTTGCCATCTGTCTGACTTGGCGAATAGAGGACCCCCCGTTTAACAGCCTTCTCTTTGCCCTGC
  TGGCCGCTGCCGGAGGTCTGCAAGGAATATACGTTCTCGTAATGCTCGTGCTTCTGATCCTCGCCTATAG
  ACGACGGTGGCGACGACTGACAGTGTGCGGCGGAATTATGTTTCTGGCTTGCGTGCTGGTTCTGATTGTC
  GACGCAGTTTTGCAGCTGAGTCCACTTCTGGGGGCCGTCACAGTCGTCAGCATGACCTTGCTGCTGCTTG
  CTTTCGTACTCTGGCTGAGCAGCCCGGGTGGGCTGGGGACACTGGGTGCCGCGCTGCTCACCCTGGCCGC
  CGCATTGGCACTGCTTGCTTCTCTTATTCTTGGCACCCTGAACTTGACCACCATGTTCCTTCTCATGCTG
  CTTTGGACACTGGTCGTTTTGCTCATATGTAGTTCTTGCTCAAGCTGCCCTCTCAGCAAGATCCTGCTCG
  CTAGACTCTTTCTCTATGCACTCGCCCTCCTGCTCCTGGCCTCTGCCCTGATTGCCGGAGGGTCTATACT
  GCAGACGAACTTCAAGAGCCTTTCCTCTACAGAGTTCATTCCAAACCTCTTTTGTATGCTTCTCCTCATC
  GTTGCCGGCATCCTCTTTATCCTGGCCATACTGACTGAATGGGGCTCTGGAAATAGAACCTACGGACCTG
  TGTTCATGTGTCTCGGGGGCCTTCTGACCATGGTGGCCGGCGCTGTATGGCTTACCGTTATGAGCAACAC
  TCTGCTGTCCGCTTGGATCCTGACTGCCGGCTTTCTCATTTTTCTCATAGGTTTTGCCCTGTTCGGTGTT
  ATTAGATGCTGCAGATATTGTTGCTACTATTGCCTCACCCTGGAGTCAGAGGAGAGACCTCCCACACCTT
  ACCGGAACACCGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101094 representing YP_401632
  Red=Cloning sites Green=Tags
  
  MNPVCLPVIVAPYLFWLAAIAASCFTASVSTVVTATGLALSLLLLAAVASSYAAAQRKLLTPVTVLTAVV
  TFFAICLTWRIEDPPFNSLLFALLAAAGGLQGIYVLVMLVLLILAYRRRWRRLTVCGGIMFLACVLVLIV
  DAVLQLSPLLGAVTVVSMTLLLLAFVLWLSSPGGLGTLGAALLTLAAALALLASLILGTLNLTTMFLLML
  LWTLVVLLICSSCSSCPLSKILLARLFLYALALLLLASALIAGGSILQTNFKSLSSTEFIPNLFCMLLLI
  VAGILFILAILTEWGSGNRTYGPVFMCLGGLLTMVAGAVWLTVMSNTLLSAWILTAGFLIFLIGFALFGV
  IRCCRYCCYYCLTLESEERPPTPYRNTV
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007605
• ORF Size: 1134 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_007605.1, YP_401632
• RefSeq ORF: 1134
• Locus ID: 3783760
• MW: 40.4 kDa