BALF3 (NC_007605) Virus Tagged ORF Clone

CAT#: VC101175

Myc-DDK-tagged ORF clone of viral ORF for BALF3 [Human herpesvirus 4], codon optimized for human cell expression, YP_401715

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BALF3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101175 represents NCBI reference of YP_401715 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGGTTTGCTGGCCGCAGCATATAGTCAGGTTTACGCCCTCGCGGTGGAACTGAGTGTGTGTACTA
  GATTGGATCCGCGCAGCCTGGACGTGGCGGCAGTGGTGAGAAATGCCGGGCTTCTTGCTGAGCTCGAGGC
  AATCCTTCTCCCAAGACTGCGACGGCAAAACGATCGCGCGTGCTCAGCTCTGTCCCTGGAACTGGTGCAC
  CTCCTGGAAAATAGCAGAGAGGCTTCTGCGGCCTTGCTTGCCCCCGGACGGAAGGGCACCAGAGTGCCTC
  CTCTGCGCACCCCTAGCGTCGCCTATAGTGTAGAATTCTACGGAGGGCACAAAGTAGACGTGTCCCTCTG
  CCTCATCAATGACATTGAAATTCTCATGAAACGGATAAATTCCGTTTTCTACTGCATGTCCCATACCATG
  GGTCTGGAAAGCCTGGAGCGAGCGCTGGATCTGCTCGGTCGCTTTCGCGGGGTGTCCCCTATCCCTGACC
  CGCGCCTGTATATTACCTCTGTGCCGTGTTGGCGATGTGTAGGAGAGCTCATGGTGCTGCCAAATCACGG
  TAATCCATCCACAGCGGAGGGGACACACGTGTCTTGCAATCACCTCGCAGTGCCCGTGAACCCTGAACCC
  GTAAGTGGCCTGTTCGAAAATGAGGTACGGCAGGCAGGACTCGGACACCTGCTGGAGGCAGAAGAGAAGG
  CTCGGCCTGGAGGCCCCGAAGAAGGGGCGGTGCCGGGTCCTGGCCGACCAGAAGCTGAAGGCGCCACAAG
  AGCCCTGGACACCTACAATGTCTTTTCCACAGTTCCACCCGAGGTTGCGGAACTGTCCGAGCTGTTGTAT
  TGGAATAGCGGCGGGCATGCTATTGGGGCAACCGGCCAGGGAGAGGGAGGGGGGCATAGCCGACTGAGCG
  CATTGTTCGCCCGGGAGCGACGCCTTGCATTGGTGAGGGGGGCATGTGAAGAAGCACTTGCCGGGGCCAG
  ACTGACGCATCTGTTTGACGCAGTCGCCCCTGGTGCCACAGAGAGGCTCTTTTGCGGGGGGGTCTACAGC
  AGCTCCGGCGACGCCGTGGAGGCTCTCAAAGCCGATTGCGCTGCTGCTTTCACAGCTCACCCCCAATATA
  GGGCCATCCTGCAAAAACGGAACGAACTTTATACTAGACTGAACCGGGCTATGCAACGCCTTGGCCGGGG
  GGAGGAAGAGGCCAGTAGAGAAAGCCCTGAAGTACCCCGGCCTGCTGGAGCCAGAGAGCCAGGTCCCAGC
  GGCGCTTTGTCAGATGCACTCAAGCGCAAAGAACAATATCTTCGGCAGGTCGCCACCGAAGGACTCGCAA
  AGCTCCAGAGCTGTCTCGCTCAGCAATCTGAGACACTGACCGAAACACTGTGTCTTAGGGTGTGGGGCGA
  CGTCGTTTACTGGGAGCTCGCACGGATGCGCAACCACTTTCTGTACCGCAGGGCATTCGTGTCCGGGCCG
  TGGGAAGACCGCAGAGCAGGAGAGGGGGCTGCATTTGAGAACTCCAAGTACATAAAAACTCACCTGTTTA
  CCCAGACTCTGAGCTCAGAGCACCTGCATGCATTGACACACTCCCTGTACACATTTATAACTGGCCCCCT
  CGCCGAAGAAAGTGGTCTGTTCCCTCCTCCCAGCAATGTAGCCCTCGCCCGCTGTTGCGATGCTGCCGGG
  ACATTGCCTCACCAGAAGGCTTTCTTGACTTCCCTGATTTGGCCTGGGATCGAGCCTTCTGACTGGATAG
  AAACTAGTTTCAATTCCTTCTATTCTGTCCCAGGCGGCTCCTTGGCATCCTCTCAGCAGATATTGTGTAG
  AGCCCTGCGGGAAGCCGTCCTCACCGTGTCCCTTTACAACAAGACTTGGGGCAGATCTCTGATATTGCGC
  CGGGCAGACGCTGTGTCTCCAGGCCAAGCCCTGCCTCCCGATGGTCTGTATCTGACCTACGACTCCGACA
  GGCCACTGATTTTGTTGTATAAAGGCCGAGGTTGGGTGTTTAAGGACCTGTATGCACTCCTGTACCTGCA
  TCTGCAAATGAGAGATGATAGTGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101175 representing YP_401715
  Red=Cloning sites Green=Tags
  
  MSGLLAAAYSQVYALAVELSVCTRLDPRSLDVAAVVRNAGLLAELEAILLPRLRRQNDRACSALSLELVH
  LLENSREASAALLAPGRKGTRVPPLRTPSVAYSVEFYGGHKVDVSLCLINDIEILMKRINSVFYCMSHTM
  GLESLERALDLLGRFRGVSPIPDPRLYITSVPCWRCVGELMVLPNHGNPSTAEGTHVSCNHLAVPVNPEP
  VSGLFENEVRQAGLGHLLEAEEKARPGGPEEGAVPGPGRPEAEGATRALDTYNVFSTVPPEVAELSELLY
  WNSGGHAIGATGQGEGGGHSRLSALFARERRLALVRGACEEALAGARLTHLFDAVAPGATERLFCGGVYS
  SSGDAVEALKADCAAAFTAHPQYRAILQKRNELYTRLNRAMQRLGRGEEEASRESPEVPRPAGAREPGPS
  GALSDALKRKEQYLRQVATEGLAKLQSCLAQQSETLTETLCLRVWGDVVYWELARMRNHFLYRRAFVSGP
  WEDRRAGEGAAFENSKYIKTHLFTQTLSSEHLHALTHSLYTFITGPLAEESGLFPPPSNVALARCCDAAG
  TLPHQKAFLTSLIWPGIEPSDWIETSFNSFYSVPGGSLASSQQILCRALREAVLTVSLYNKTWGRSLILR
  RADAVSPGQALPPDGLYLTYDSDRPLILLYKGRGWVFKDLYALLYLHLQMRDDSA
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007605
• ORF Size: 2055 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_007605.1, YP_401715
• RefSeq ORF: 2055
• Locus ID: 3783679
• MW: 74.8 kDa