52K (AC_000007) Virus Tagged ORF Clone

CAT#: VC100037

Myc-DDK-tagged ORF clone of viral ORF for 52K [Human adenovirus 2], codon optimized for human cell expression, AP_000168

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 52K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100037 represents NCBI reference of AP_000168 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACCCTGTGCTGCGCCAAATGAGGCCTCCTCCCCAGCAGCGGCAAGAGCAAGAACAAAGGCAGACCT
  GTAGAGCACCATCACCCAGCCCAACAGCTTCCGGGGGAGCAACAAGCGCGGCAGATGCAGCCGCTGATGG
  CGACTATGAGCCACCTCGACGACGGGCTCGCCATTATCTCGACCTGGAGGAAGGAGAGGGACTGGCACGG
  CTGGGGGCACCTTCCCCAGAACGGCATCCACGGGTGCAGCTCAAGAGGGACACCCGGGAGGCTTACGTCC
  CTAGACAGAACCTCTTTCGCGACCGAGAGGGGGAGGAACCAGAGGAGATGCGGGACAGGAAATTCCACGC
  AGGTAGGGAACTGCGGCATGGCCTGAATAGGGAACGGTTGCTGCGAGAAGAAGACTTCGAGCCTGACGCC
  AGGACCGGGATTTCACCCGCCAGAGCCCACGTTGCTGCTGCAGATCTGGTGACAGCTTACGAGCAGACAG
  TCAACCAGGAGATTAATTTTCAAAAATCCTTTAACAATCACGTCAGGACTCTGGTTGCACGCGAAGAAGT
  GGCAATCGGACTCATGCATCTGTGGGATTTCGTGTCAGCACTCGAGCAAAACCCAAATTCTAAGCCGCTC
  ATGGCTCAGCTGTTCCTGATTGTGCAGCACTCCCGGGATAATGAGGCCTTCAGAGATGCTCTGCTGAATA
  TAGTTGAGCCCGAAGGAAGATGGCTGCTGGACCTGATCAACATCCTGCAGTCCATTGTGGTGCAGGAGCG
  GAGCCTCTCCCTTGCCGACAAAGTTGCCGCGATCAACTATAGCATGCTGTCCCTGGGGAAATTCTATGCA
  CGGAAAATTTACCACACACCATACGTGCCCATCGATAAGGAAGTCAAGATAGAGGGATTTTACATGCGCA
  TGGCCCTGAAAGTTCTGACGCTCTCTGATGACCTGGGCGTTTACAGAAACGAGCGGATCCATAAGGCAGT
  GAGTGTTTCACGGCGCAGAGAGCTGTCAGATCGCGAGCTCATGCACAGCTTGCAGAGAGCCCTGGCCGGC
  ACGGGCAGCGGGGACAGAGAGGCCGAGAGCTATTTTGACGCTGGAGCAGATCTGAGATGGGCTCCTAGCC
  GACGAGCATTGGAGGCAGCAGGTGCAGGCCCTGGTCTGGCTGTCGCACCAGCTAGAGCTGGCAACGTGGG
  AGGTGTGGAGGAGTATGATGAAGACGACGAATACGAGCCTGAAGATGGCGAATAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100037 representing AP_000168
  Red=Cloning sites Green=Tags
  
  MHPVLRQMRPPPQQRQEQEQRQTCRAPSPSPTASGGATSAADAAADGDYEPPRRRARHYLDLEEGEGLAR
  LGAPSPERHPRVQLKRDTREAYVPRQNLFRDREGEEPEEMRDRKFHAGRELRHGLNRERLLREEDFEPDA
  RTGISPARAHVAAADLVTAYEQTVNQEINFQKSFNNHVRTLVAREEVAIGLMHLWDFVSALEQNPNSKPL
  MAQLFLIVQHSRDNEAFRDALLNIVEPEGRWLLDLINILQSIVVQERSLSLADKVAAINYSMLSLGKFYA
  RKIYHTPYVPIDKEVKIEGFYMRMALKVLTLSDDLGVYRNERIHKAVSVSRRRELSDRELMHSLQRALAG
  TGSGDREAESYFDAGADLRWAPSRRALEAAGAGPGLAVAPARAGNVGGVEEYDEDDEYEPEDGEY
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000007
• ORF Size: 1245 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000007.1, AP_000168
• RefSeq ORF: 1245
• MW: 47.0 kDa