IVa2 (AC_000007) Virus Tagged ORF Clone

CAT#: VC100058

Myc-DDK-tagged ORF clone of viral ORF for IVa2 [Human adenovirus 2], codon optimized for human cell expression, AP_000165

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IVa2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100058 represents NCBI reference of AP_000165 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAACCAGAGGCCGCCGCCCAGCTGCACTGCAGCACCAACAGGATCAACCTCAGGCTCACCCCGGCC
  AGAGAGCTGCGAGATCTGCACCACTGCACCGCGACCCAGATTATGCCGATGAGGACCCCGCCCCGGTCGA
  GAGACACGATCCAGGCCCTTCCGGAAGAGCACCAACTACCGCGGTGCAGCGAAAGCCGCCACAACCCGCC
  AAGCGCGGCGATATGTTGGACAGAGATGCAGTGGAGCACGTAACAGAACTTTGGGACAGGCTGGAGCTGC
  TTGGCCAGACACTGAAATCAATGCCTACCGCTGATGGCCTCAAACCCCTTAAAAACTTCGCCTCCTTGCA
  GGAGCTTCTCTCCCTCGGGGGCGAGCGGCTGCTGGCGCACCTGGTCAGGGAGAATATGCAGGTGCGCGAT
  ATGCTCAACGAGGTCGCTCCACTGCTCAGAGACGATGGCAGTTGTAGTTCACTGAATTACCAACTCCAGC
  CCGTCATCGGTGTGATCTATGGCCCCACTGGGTGCGGAAAGTCACAACTGCTTAGGAACCTGCTGTCTTC
  ACAGCTGATTTCACCCACCCCGGAAACAGTCTTCTTTATCGCGCCTCAGGTCGACATGATACCGCCGTCT
  GAACTGAAGGCTTGGGAGATGCAAATATGTGAGGGCAATTATGCACCTGGGCCTGATGGCACAATCATCC
  CTCAGTCTGGGACCCTCCGGCCGCGGTTTGTCAAGATGGCCTACGATGACCTGATCCTTGAGCATAACTA
  CGATGTCAGCGATCCTCGAAATATCTTTGCACAAGCAGCAGCCAGAGGCCCAATCGCGATTATCATGGAT
  GAGTGCATGGAGAACCTGGGCGGACATAAAGGAGTTTCCAAATTTTTCCACGCTTTCCCTTCAAAGCTGC
  ACGATAAATTTCCGAAGTGCACCGGATATACCGTACTGGTCGTACTCCATAATATGAACCCGCGGCGGGA
  TATGGCCGGTAATATAGCTAATCTGAAAATTCAGTCCAAGATGCACCTGATCAGCCCCCGCATGCACCCT
  TCACAGCTGAATAGGTTTGTGAACACCTATACAAAGGGCCTGCCCCTTGCCATCTCTTTGCTTCTCAAGG
  ATATTTTCAGACATCATGCTCAGAGAAGTTGCTATGACTGGATCATTTACAACACTACTCCTCAACATGA
  AGCCCTCCAGTGGTGCTACCTTCACCCACGCGATGGACTGATGCCAATGTACCTGAATATCCAGTCACAC
  CTTTACCACGTGCTTGAGAAGATCCACAGGACGCTGAACGACAGAGATCGGTGGTCCAGAGCCTACCGGG
  CTCGGAAGACTCCTAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100058 representing AP_000165
  Red=Cloning sites Green=Tags
  
  METRGRRPAALQHQQDQPQAHPGQRAARSAPLHRDPDYADEDPAPVERHDPGPSGRAPTTAVQRKPPQPA
  KRGDMLDRDAVEHVTELWDRLELLGQTLKSMPTADGLKPLKNFASLQELLSLGGERLLAHLVRENMQVRD
  MLNEVAPLLRDDGSCSSLNYQLQPVIGVIYGPTGCGKSQLLRNLLSSQLISPTPETVFFIAPQVDMIPPS
  ELKAWEMQICEGNYAPGPDGTIIPQSGTLRPRFVKMAYDDLILEHNYDVSDPRNIFAQAAARGPIAIIMD
  ECMENLGGHKGVSKFFHAFPSKLHDKFPKCTGYTVLVVLHNMNPRRDMAGNIANLKIQSKMHLISPRMHP
  SQLNRFVNTYTKGLPLAISLLLKDIFRHHAQRSCYDWIIYNTTPQHEALQWCYLHPRDGLMPMYLNIQSH
  LYHVLEKIHRTLNDRDRWSRAYRARKTPK
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000007
• ORF Size: 1347 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000007.1, AP_000165
• RefSeq ORF: 1347
• MW: 50.9 kDa