E3 gp19K (AC_000007) Virus Tagged ORF Clone

CAT#: VC100062

Myc-DDK-tagged ORF clone of viral ORF for E3 gp19K [Human adenovirus 2], codon optimized for human cell expression, AP_000184

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E3 gp19K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100062 represents NCBI reference of AP_000184 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGTACATGATTTTGGGTCTGCTGGCACTGGCCGCCGTCTGCAGCGCAGCCAAAAAAGTCGAATTCA
  AGGAACCCGCGTGTAATGTGACGTTTAAGTCCGAAGCAAATGAATGTACCACCCTCATAAAGTGCACTAC
  AGAGCACGAAAAGTTGATCATTAGACATAAAGATAAAATAGGAAAATATGCTGTTTATGCCATCTGGCAG
  CCGGGAGACACCAATGACTACAACGTAACAGTTTTTCAGGGAGAGAACAGAAAGACATTTATGTATAAAT
  TTCCATTCTACGAGATGTGCGACATTACAATGTATATGTCTAAGCAATATAAGCTTTGGCCTCCTCAAAA
  GTGTCTGGAAAACACTGGAACGTTCTGTAGCACGGCCCTCCTTATAACAGCCCTGGCCCTCGTTTGTACA
  CTCCTGTACCTGAAGTACAAAAGCCGCAGATCCTTCATCGATGAGAAGAAGATGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100062 representing AP_000184
  Red=Cloning sites Green=Tags
  
  MRYMILGLLALAAVCSAAKKVEFKEPACNVTFKSEANECTTLIKCTTEHEKLIIRHKDKIGKYAVYAIWQ
  PGDTNDYNVTVFQGENRKTFMYKFPFYEMCDITMYMSKQYKLWPPQKCLENTGTFCSTALLITALALVCT
  LLYLKYKSRRSFIDEKKMP
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000007
• ORF Size: 477 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000007.1, AP_000184
• RefSeq ORF: 477
• MW: 18.4 kDa