pVIII (AC_000007) Virus Tagged ORF Clone

CAT#: VC100050

Myc-DDK-tagged ORF clone of viral ORF for pVIII [Human adenovirus 2], codon optimized for human cell expression, AP_000181

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: pVIII
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100050 represents NCBI reference of AP_000181 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTAAAGAAATACCAACACCATACATGTGGAGCTACCAACCTCAGATGGGCTTGGCGGCAGGGGCTG
  CACAGGACTACTCCACCCGCATCAACTACATGAGTGCAGGGCCACATATGATATCTAGGGTGAACGGGAT
  CAGGGCTCACAGAAATCGGATTCTGCTTGAACAGGCAGCTATTACCACCACCCCTCGGAATAACCTCAAT
  CCGAGGAGCTGGCCTGCAGCGCTCGTGTACCAGGAATCTCCTGCACCTACAACAGTCGTCCTGCCTCGCG
  ACGCCCAGGCTGAGGTGCAAATGACAAATAGCGGCGCTCAGCTGGCCGGAGGCTTCCGACATAGGGTGAG
  GTCTCCCGGGCAGGGCATTACTCACCTGAAAATCCGAGGGCGAGGGATCCAGCTGAATGACGAATCTGTG
  AGTAGTAGTCTGGGCCTCAGACCCGATGGTACCTTCCAGATAGGAGGAGCCGGACGCTCTAGCTTCACTC
  CAAGACAAGCCATCCTTACACTTCAAACCTCCTCAAGCGAGCCCCGCAGTGGCGGCATCGGAACACTGCA
  GTTTATCGAGGAGTTCGTGCCCAGCGTGTACTTTAATCCTTTTTCTGGGCCTCCGGGACACTATCCTGAC
  CAGTTTATCCCTAATTTCGACGCCGTGAAGGATTCAGCAGACGGCTACGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100050 representing AP_000181
  Red=Cloning sites Green=Tags
  
  MSKEIPTPYMWSYQPQMGLAAGAAQDYSTRINYMSAGPHMISRVNGIRAHRNRILLEQAAITTTPRNNLN
  PRSWPAALVYQESPAPTTVVLPRDAQAEVQMTNSGAQLAGGFRHRVRSPGQGITHLKIRGRGIQLNDESV
  SSSLGLRPDGTFQIGGAGRSSFTPRQAILTLQTSSSEPRSGGIGTLQFIEEFVPSVYFNPFSGPPGHYPD
  QFIPNFDAVKDSADGYD
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000007
• ORF Size: 681 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000007.1, AP_000181
• RefSeq ORF: 681
• MW: 24.7 kDa