22K (AC_000007) Virus Tagged ORF Clone

CAT#: VC100049

Myc-DDK-tagged ORF clone of viral ORF for 22K [Human adenovirus 2], codon optimized for human cell expression, AP_000180

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 22K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100049 represents NCBI reference of AP_000180 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCTAAGAAGAAATTGCAGCTGCCCCCCCCTCCACCTACGGACGAGGAGGAATATTGGGACTCCC
  AAGCTGAGGAGGTCCTGGATGAAGAGGAAGAAATGATGGAAGATTGGGACTCCCTTGATGAAGCCAGCGA
  AGCCGAGGAGGTGTCCGACGAAACCCCTTCCCCAAGTGTCGCCTTCCCTTCACCAGCACCACAGAAGCTG
  GCCACAGTACCCTCTATTGCTACCACCAGTGCACCGCAGGCACCGCCAGCACTTCCTGTCCGCCGCCCAA
  ACCGACGATGGGACACCACAGGGACCCGAGCAGGAAAGAGTAAGCAGCCTCCACCTCTCGCACAGGAACA
  GCAGCAGCGGCAGGGTTATAGAAGTTGGAGGGGTCACAAGAATGCGATTGTGGCTTGTCTGCAGGATTGC
  GGCGGCAACATTTCCTTTGCCCGGCGCTTTCTCTTGTATCACCACGGAGTGGCCTTTCCCCGCAATATTC
  TCCATTACTACCGGCATCTCTATTCTCCCTACTGTACCGGAGGATCCGGCAGTGGGTCAAATTCTTCTGG
  GCACACCGAAGCGAAGGCCACAGGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100049 representing AP_000180
  Red=Cloning sites Green=Tags
  
  MAPKKKLQLPPPPPTDEEEYWDSQAEEVLDEEEEMMEDWDSLDEASEAEEVSDETPSPSVAFPSPAPQKL
  ATVPSIATTSAPQAPPALPVRRPNRRWDTTGTRAGKSKQPPPLAQEQQQRQGYRSWRGHKNAIVACLQDC
  GGNISFARRFLLYHHGVAFPRNILHYYRHLYSPYCTGGSGSGSNSSGHTEAKATG
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000007
• ORF Size: 585 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000007.1, AP_000180
• RefSeq ORF: 585
• MW: 21.5 kDa