E1A (AC_000007) Virus Tagged ORF Clone

CAT#: VC100054

Myc-DDK-tagged ORF clone of viral ORF for E1A [Human adenovirus 2], codon optimized for human cell expression, AP_000161

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100054 represents NCBI reference of AP_000161 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGACACATTATCTGCCACGGGGGTGTCATCACTGAGGAGATGGCGGCCAGCCTTCTGGATCAGCTGA
  TAGAGGAAGTACTTGCCGACAACCTCCCTCCTCCCTCACATTTTGAGCCACCAACCTTGCACGAGCTGTA
  CGATCTGGATGTTACGGCCCCCGAGGACCCCAACGAAGAAGCCGTATCTCAGATCTTTCCTGAGTCTGTG
  ATGCTCGCTGTGCAAGAAGGCATTGACTTGTTTACGTTCCCTCCAGCCCCCGGGTCACCCGAGCCCCCTC
  ACTTGAGCCGGCAACCCGAGCAACCAGAGCAGCGCGCCTTGGGTCCGGTGAGCATGCCAAATCTGGTGCC
  AGAGGTCATCGATCTGACTTGTCACGAGGCCGGCTTTCCCCCCAGCGACGACGAGGATGAGGAAGGCGAA
  GAATTTGTGCTGGACTATGTCGAGCACCCCGGGCACGGGTGTCGCAGTTGCCATTACCATCGGCGAAACA
  CAGGCGACCCAGACATTATGTGTAGCTTGTGTTATATGAGAACTTGTGGCATGTTTGTGTATAGCCCAGT
  GAGTGAACCCGAGCCCGAGCCTGAGCCCGAACCCGAGCCTGCGCGACCCACGCGGCGACCAAAACTGGTC
  CCAGCCATTCTGCGCCGACCAACATCCCCAGTGAGCCGGGAATGTAACAGCAGCACGGATAGTTGTGACT
  CAGGTCCTAGCAATACCCCTCCCGAAATCCACCCTGTGGTGCCTCTTTGCCCTATTAAGCCCGTTGCTGT
  GCGGGTAGGCGGACGAAGACAGGCCGTCGAATGCATTGAAGATCTTCTGAACGAGTCAGGACAACCACTG
  GATCTGTCATGCAAAAGACCGAGGCCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100054 representing AP_000161
  Red=Cloning sites Green=Tags
  
  MRHIICHGGVITEEMAASLLDQLIEEVLADNLPPPSHFEPPTLHELYDLDVTAPEDPNEEAVSQIFPESV
  MLAVQEGIDLFTFPPAPGSPEPPHLSRQPEQPEQRALGPVSMPNLVPEVIDLTCHEAGFPPSDDEDEEGE
  EFVLDYVEHPGHGCRSCHYHRRNTGDPDIMCSLCYMRTCGMFVYSPVSEPEPEPEPEPEPARPTRRPKLV
  PAILRRPTSPVSRECNSSTDSCDSGPSNTPPEIHPVVPLCPIKPVAVRVGGRRQAVECIEDLLNESGQPL
  DLSCKRPRP
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000007
• ORF Size: 867 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000007.1, AP_000161
• RefSeq ORF: 867
• MW: 31.9 kDa