pTP (AC_000007) Virus Tagged ORF Clone

CAT#: VC100057

Myc-DDK-tagged ORF clone of viral ORF for pTP [Human adenovirus 2], codon optimized for human cell expression, AP_000167

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: pTP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100057 represents NCBI reference of AP_000167 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTTGAGCGTCAACGACTGCGCCCGGCTTACTGGACAGAGCGTCCCCACCATGGAGCACTTTTTGC
  CCCTGAGAAACATCTGGAACCGCGTCCGGGATTTTCCCAGGGCATCCACAACCGCAGCTGGCATTACTTG
  GATGAGTCGCTACATCTATGGTTACCATAGGTTGATGCTGGAAGATCTCGCTCCAGGTGCCCCCGCAACC
  CTGAGATGGCCACTGTACCGGCAGCCCCCTCCACACTTCCTGGTCGGTTATCAGTACTTGGTGCGAACTT
  GTAATGACTATGTTTTCGATTCCAGGGCCTATTCTCGACTGCGGTATACTGAGCTGTCTCAACCCGGACA
  TCAGACTGTGAACTGGAGCGTTATGGCTAATTGTACCTACACTATCAATACCGGTGCCTATCACCGATTC
  GTGGACATGGACGACTTTCAGAGCACACTCACACAGGTGCAGCAGGCCATTTTGGCAGAACGAGTCGTCG
  CCGACCTCGCACTCCTCCAGCCTATGCGAGGGTTCGGAGTCACACGGATGGGCGGTCGGGGCAGGCACCT
  GCGCCCAAATTCCGCTGCAGCTGTCGCGATTGATGCTAGAGACGCTGGTCAGGAGGAGGGTGAAGAAGAA
  GTGCCTGTGGAGAGGCTTATGCAGGATTACTACAAGGATCTGAGGCGCTGCCAAAACGAAGCCTGGGGCA
  TGGCAGACCGGCTGCGCATTCAGCAAGCTGGGCCTAAGGATATGGTGCTTCTCTCTACCATCAGGCGCCT
  GAAAACCGCATATTTCAATTATATTATCAGTAGCACCTCCGCGCGGAACAACCCCGACAGGCATCCCCTG
  CCCCCCGCCACCGTGTTGTCCCTGCCTTGTGATTGCGATTGGTTGGACGCATTTCTCGAGCGATTCTCCG
  ACCCAGTTGACGCCGACAGCCTGAGATCCCTGGGCGGCGGCGTTCCCACACAGCAACTCCTCAGATGTAT
  TGTCAGTGCTGTCTCTCTGCCCCATGGGTCACCACCCCCCACACACAACCGGGATATGACAGGAGGCGTT
  TTCCAGCTTAGACCCAGGGAGAACGGCAGGGCTGTCACAGAGACAATGAGGCGCCGGAGGGGAGAAATGA
  TTGAGCGGTTCGTGGACAGATTGCCCGTGCGACGGCGGAGGAGACGAGTGCCACCTCCACCCCCGCCCCC
  GGAAGAAGAGGAGGAAGGCGAAGCACTTATGGAGGAAGAAATCGAAGAGGAGGAGGCCCCCGTGGCCTTC
  GAAAGGGAAGTGAGGGACACCGTCGCAGAACTGATCCGCTTGCTGGAGGAGGAGCTCACGGTGTCTGCAC
  GCAACTCTCAATTCTTCAATTTCGCCGTCGATTTCTACGAAGCTATGGAAAGGCTTGAAGCTCTGGGTGA
  CATCAATGAAAGTACCCTCCGGAGGTGGGTAATGTACTTTTTTGTGGCTGAACACACAGCCACCACACTG
  AACTACCTGTTTCAGAGACTGAGGAATTACGCCGTGTTCGCCAGACACGTTGAGCTGAATCTTGCTCAGG
  TAGTGATGCGCGCCAGAGACGCTGAAGGGGGCGTGGTCTATTCTCGAGTGTGGAACGAGGGAGGACTGAA
  TGCCTTCTCCCAGCTGATGGCCAGGATTAGCAATGATCTGGCCGCCACCGTCGAGCGGGCAGGTAGGGGC
  GACCTGCAAGAGGAAGAGATCGAACAGTTCATGGCCGAGATCGCCTATCAGGACAATTCAGGCGACGTCC
  AAGAAATCCTCCGGCAGGCTGCTGTGAATGACACCGAAATCGACTCTGTGGAATTGAGCTTCCGCTTTAA
  GCTCACCGGGCCTGTGGTGTTCACACAGAGAAGACAGATCCAGGAAATTAACCGGAGGGTAGTCGCATTC
  GCCTCAAATCTGCGCGCACAACATCAGTTGCTGCCTGCAAGGGGGGCCGATGTGCCTCTGCCTCCATTGC
  CTGCTGGTCCCGAACCCCCCCTGCCCCCCGGAGCACGCCCTAGACATCGCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100057 representing AP_000167
  Red=Cloning sites Green=Tags
  
  MALSVNDCARLTGQSVPTMEHFLPLRNIWNRVRDFPRASTTAAGITWMSRYIYGYHRLMLEDLAPGAPAT
  LRWPLYRQPPPHFLVGYQYLVRTCNDYVFDSRAYSRLRYTELSQPGHQTVNWSVMANCTYTINTGAYHRF
  VDMDDFQSTLTQVQQAILAERVVADLALLQPMRGFGVTRMGGRGRHLRPNSAAAVAIDARDAGQEEGEEE
  VPVERLMQDYYKDLRRCQNEAWGMADRLRIQQAGPKDMVLLSTIRRLKTAYFNYIISSTSARNNPDRHPL
  PPATVLSLPCDCDWLDAFLERFSDPVDADSLRSLGGGVPTQQLLRCIVSAVSLPHGSPPPTHNRDMTGGV
  FQLRPRENGRAVTETMRRRRGEMIERFVDRLPVRRRRRRVPPPPPPPEEEEEGEALMEEEIEEEEAPVAF
  EREVRDTVAELIRLLEEELTVSARNSQFFNFAVDFYEAMERLEALGDINESTLRRWVMYFFVAEHTATTL
  NYLFQRLRNYAVFARHVELNLAQVVMRARDAEGGVVYSRVWNEGGLNAFSQLMARISNDLAATVERAGRG
  DLQEEEIEQFMAEIAYQDNSGDVQEILRQAAVNDTEIDSVELSFRFKLTGPVVFTQRRQIQEINRRVVAF
  ASNLRAQHQLLPARGADVPLPPLPAGPEPPLPPGARPRHRF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000007
• ORF Size: 2013 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000007.1, AP_000167
• RefSeq ORF: 2013
• MW: 76.5 kDa