33K (AC_000007) Virus Tagged ORF Clone

CAT#: VC100048

Myc-DDK-tagged ORF clone of viral ORF for 33K [Human adenovirus 2], codon optimized for human cell expression, AP_000179

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 33K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100048 represents NCBI reference of AP_000179 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCCTAAAAAAAAGCTGCAGCTCCCCCCGCCCCCTCCAACAGATGAGGAGGAGTACTGGGATAGTC
  AGGCTGAAGAGGTGTTGGACGAAGAAGAGGAAATGATGGAGGATTGGGACTCCCTGGACGAGGCCTCTGA
  GGCTGAAGAGGTATCAGACGAAACGCCATCTCCCAGCGTGGCCTTCCCATCTCCTGCCCCACAGAAACTG
  GCCACGGTACCATCCATAGCTACCACTTCCGCCCCACAGGCCCCTCCCGCCCTCCCTGTCAGACGACCAA
  ATCGGCGGTGGGACACGACAGGAACTAGAGCTGCTCCTACTGCACCCGCAGCAGCTGCTGCAGCTGCTAC
  AGCCGCCGTCACACAGAAGCAGAGAAGACCTGACTCCAAAACCCTGACTAAGCCGAAAAAGTCCACCGCG
  GCTGCGGCGGCAGGGGGCGGCGCGCTGAGACTGGCCCCAAACGAGCCTGTGAGCACTAGAGAGCTGAGAA
  ACCGCATTTTCCCAACCCTCTACGCAATCTTCCAGCAATCTCGGGGTCAGGAACAGGAATTGAAAATTAA
  GAACCGGAGCCTTCGAAGCTTGACCAGATCATGCCTTTACCACAAGTCCGAAGACCAGCTGAGGAGAACA
  CTGGAGGACGCTGAGGCTCTCTTCTCTAAGTACTGCGCCCTGACCCTGAAGGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100048 representing AP_000179
  Red=Cloning sites Green=Tags
  
  MAPKKKLQLPPPPPTDEEEYWDSQAEEVLDEEEEMMEDWDSLDEASEAEEVSDETPSPSVAFPSPAPQKL
  ATVPSIATTSAPQAPPALPVRRPNRRWDTTGTRAAPTAPAAAAAAATAAVTQKQRRPDSKTLTKPKKSTA
  AAAAGGGALRLAPNEPVSTRELRNRIFPTLYAIFQQSRGQEQELKIKNRSLRSLTRSCLYHKSEDQLRRT
  LEDAEALFSKYCALTLKD
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000007
• ORF Size: 684 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000007.1, AP_000179
• RefSeq ORF: 684
• MW: 25.0 kDa