hexon (AC_000007) Virus Tagged ORF Clone

CAT#: VC100044

Myc-DDK-tagged ORF clone of viral ORF for hexon [Human adenovirus 2], codon optimized for human cell expression, AP_000175

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: hexon
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100044 represents NCBI reference of AP_000175 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACCCCATCAATGATGCCCCAGTGGTCCTATATGCATATCAGCGGGCAGGACGCAAGCGAGTATC
  TCAGCCCCGGCCTCGTGCAGTTCGCCCGGGCAACCGAAACCTATTTTTCCCTGAATAACAAGTTCAGAAA
  CCCCACCGTAGCTCCTACGCACGACGTCACTACTGACCGAAGCCAGAGACTGACACTTCGCTTTATCCCT
  GTCGACCGCGAAGATACTGCTTACTCCTACAAGGCACGCTTCACTCTTGCCGTTGGAGACAACCGGGTTT
  TGGACATGGCAAGCACATACTTTGACATTAGAGGTGTCCTGGACAGGGGACCCACCTTCAAACCTTATTC
  TGGCACCGCTTATAACGCATTGGCCCCGAAAGGAGCCCCTAATAGCTGTGAATGGGAACAAACAGAAGAT
  AGTGGCAGGGCCGTTGCTGAGGACGAGGAGGAGGAGGACGAAGACGAGGAAGAGGAAGAGGAAGAGCAGA
  ACGCTAGAGATCAAGCTACGAAGAAGACACATGTCTACGCACAGGCACCTCTGAGCGGGGAAACAATTAC
  AAAGTCCGGCCTCCAGATCGGCTCAGACAACGCTGAAACCCAGGCTAAACCAGTTTACGCCGATCCCTCC
  TACCAGCCCGAGCCACAGATCGGCGAATCTCAGTGGAACGAAGCGGACGCCAACGCCGCTGGTGGTCGAG
  TGCTGAAAAAGACTACTCCCATGAAACCGTGCTATGGCTCCTACGCTAGACCTACCAACCCATTCGGTGG
  GCAGTCCGTCCTGGTGCCTGACGAAAAGGGCGTGCCGCTGCCTAAGGTTGACCTGCAATTCTTCTCAAAC
  ACTACATCCCTGAACGATCGGCAAGGCAACGCGACTAAACCTAAGGTTGTCCTTTATTCAGAGGACGTGA
  ATATGGAAACCCCGGATACTCATCTGAGCTACAAGCCAGGAAAAGGCGATGAGAACAGTAAAGCAATGCT
  TGGACAACAGTCAATGCCTAACAGACCCAACTACATTGCGTTTCGCGACAATTTTATTGGCCTGATGTAT
  TACAATTCCACAGGCAATATGGGCGTCTTGGCTGGACAGGCAAGCCAGCTCAACGCCGTAGTGGATCTGC
  AGGACAGGAACACGGAACTGAGCTACCAGCTCCTTCTTGACTCCATCGGCGACCGGACCCGCTATTTCAG
  TATGTGGAACCAGGCGGTTGATTCCTATGACCCCGACGTGAGAATAATAGAGAACCACGGTACTGAGGAT
  GAGTTGCCAAACTACTGCTTCCCATTGGGAGGAATCGGAGTGACCGACACGTATCAGGCCATAAAAGCCA
  ATGGCAATGGATCAGGCGATAATGGCGACACGACATGGACTAAGGATGAGACTTTCGCCACTCGGAATGA
  GATCGGCGTAGGTAACAACTTCGCCATGGAAATTAATTTGAACGCTAATCTGTGGCGGAATTTTCTGTAC
  TCTAATATAGCCCTGTATCTGCCAGATAAGCTGAAATACAACCCCACAAACGTAGAAATCTCCGACAATC
  CTAACACTTATGACTACATGAACAAGAGGGTGGTGGCCCCAGGCCTGGTGGACTGTTACATCAACCTGGG
  CGCCCGCTGGTCTCTGGATTACATGGACAATGTGAATCCTTTTAACCACCACAGAAATGCTGGGCTGCGC
  TACCGGAGCATGCTGCTCGGTAATGGACGATACGTGCCTTTTCACATTCAGGTGCCCCAAAAGTTTTTCG
  CAATTAAAAATCTGCTCCTCCTGCCAGGTTCTTATACTTATGAGTGGAACTTCAGGAAAGATGTGAACAT
  GGTGCTGCAATCCTCCCTTGGTAATGACTTGCGGGTGGACGGGGCCTCCATTAAGTTTGACTCCATCTGC
  CTGTACGCAACTTTCTTTCCCATGGCACACAATACCGCTTCTACGCTCGAGGCGATGCTGAGGAACGACA
  CTAATGACCAGAGCTTTAATGATTACTTGAGCGCTGCTAACATGCTCTACCCCATTCCAGCAAACGCCAC
  TAACGTACCTATCAGCATTCCATCTCGGAACTGGGCGGCATTCAGAGGCTGGGCTTTTACCCGCCTCAAA
  ACCAAAGAGACTCCCTCCCTCGGGTCTGGCTACGATCCCTATTACACATACTCCGGCTCCATCCCTTACC
  TTGACGGAACTTTCTACCTTAACCACACCTTTAAAAAAGTGGCTATAACTTTCGACTCCTCTGTGTCATG
  GCCCGGGAATGACCGGCTTCTGACTCCAAATGAATTTGAGATCAAGAGAAGCGTGGATGGAGAGGGTTAT
  AATGTGGCGCAGTGCAACATGACCAAGGATTGGTTTCTTGTGCAGATGCTGGCCAACTATAACATCGGCT
  ACCAGGGGTTTTACATACCCGAATCTTACAAAGACAGGATGTACTCATTTTTCCGAAATTTTCAACCAAT
  GTCAAGGCAGGTTGTGGACGATACCAAATATAAAGAGTACCAGCAGGTCGGTATACTGCACCAACACAAC
  AATTCAGGTTTTGTGGGCTATCTGGCCCCAACCATGAGAGAGGGCCAGGCCTACCCTGCCAATGTTCCGT
  ATCCGTTGATTGGCAAGACGGCCGTAGATTCTATAACCCAGAAGAAATTCCTCTGCGATAGAACACTTTG
  GCGGATTCCCTTTTCTAGCAATTTCATGTCCATGGGTGCCCTGACGGATTTGGGTCAGAATCTTCTGTAT
  GCCAATAGTGCTCATGCCCTTGACATGACTTTTGAGGTGGACCCCATGGATGAACCAACCCTCCTTTACG
  TTCTGTTCGAAGTTTTCGATGTGGTGCGCGTCCATCAGCCCCACAGAGGGGTGATCGAGACCGTCTATCT
  GCGGACACCATTCTCAGCCGGCAACGCAACTACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100044 representing AP_000175
  Red=Cloning sites Green=Tags
  
  MATPSMMPQWSYMHISGQDASEYLSPGLVQFARATETYFSLNNKFRNPTVAPTHDVTTDRSQRLTLRFIP
  VDREDTAYSYKARFTLAVGDNRVLDMASTYFDIRGVLDRGPTFKPYSGTAYNALAPKGAPNSCEWEQTED
  SGRAVAEDEEEEDEDEEEEEEEQNARDQATKKTHVYAQAPLSGETITKSGLQIGSDNAETQAKPVYADPS
  YQPEPQIGESQWNEADANAAGGRVLKKTTPMKPCYGSYARPTNPFGGQSVLVPDEKGVPLPKVDLQFFSN
  TTSLNDRQGNATKPKVVLYSEDVNMETPDTHLSYKPGKGDENSKAMLGQQSMPNRPNYIAFRDNFIGLMY
  YNSTGNMGVLAGQASQLNAVVDLQDRNTELSYQLLLDSIGDRTRYFSMWNQAVDSYDPDVRIIENHGTED
  ELPNYCFPLGGIGVTDTYQAIKANGNGSGDNGDTTWTKDETFATRNEIGVGNNFAMEINLNANLWRNFLY
  SNIALYLPDKLKYNPTNVEISDNPNTYDYMNKRVVAPGLVDCYINLGARWSLDYMDNVNPFNHHRNAGLR
  YRSMLLGNGRYVPFHIQVPQKFFAIKNLLLLPGSYTYEWNFRKDVNMVLQSSLGNDLRVDGASIKFDSIC
  LYATFFPMAHNTASTLEAMLRNDTNDQSFNDYLSAANMLYPIPANATNVPISIPSRNWAAFRGWAFTRLK
  TKETPSLGSGYDPYYTYSGSIPYLDGTFYLNHTFKKVAITFDSSVSWPGNDRLLTPNEFEIKRSVDGEGY
  NVAQCNMTKDWFLVQMLANYNIGYQGFYIPESYKDRMYSFFRNFQPMSRQVVDDTKYKEYQQVGILHQHN
  NSGFVGYLAPTMREGQAYPANVPYPLIGKTAVDSITQKKFLCDRTLWRIPFSSNFMSMGALTDLGQNLLY
  ANSAHALDMTFEVDPMDEPTLLYVLFEVFDVVRVHQPHRGVIETVYLRTPFSAGNATT
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000007
• ORF Size: 2904 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000007.1, AP_000175
• RefSeq ORF: 2904
• MW: 109.2 kDa