100K (AC_000007) Virus Tagged ORF Clone

CAT#: VC100047

Myc-DDK-tagged ORF clone of viral ORF for 100K [Human adenovirus 2], codon optimized for human cell expression, AP_000178

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 100K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100047 represents NCBI reference of AP_000178 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAATCTGTGGAAAAAGAAGATAGTCTGACTGCTCCATTCGAGTTCGCAACCACCGCCAGTACTGATG
  CCGCTAATGCACCCACAACCTTTCCCGTGGAGGCCCCACCCCTTGAGGAAGAGGAAGTTATAATTGAACA
  GGATCCTGGGTTTGTTAGCGAGGACGATGAAGATCGGTCCGTACCCACAGAAGACAAGAAGCAGGACCAG
  GACGATGCCGAGGCCAATGAGGAGCAGGTCGGGAGAGGCGACCAGCGGCACGGTGACTACCTGGATGTTG
  GCGATGATGTCTTGCTGAAGCATCTGCAGCGCCAGTGTGCGATAATTTGTGATGCTTTGCAGGAACGAAG
  CGATGTCCCATTGGCCATCGCGGACGTTTCCCTGGCATACGAGCGCCACCTGTTCAGCCCTAGAGTGCCT
  CCCAAGCGACAGGAGAACGGGACTTGTGAGCCAAACCCGCGGCTGAATTTCTACCCGGTCTTCGCCGTTC
  CTGAAGTGCTTGCAACCTATCACATATTCTTCCAGAATTGCAAGATCCCTCTCAGCTGTAGAGCCAACAG
  ATCAAGAGCCGATAAACAGCTCGCTCTGCGCCAGGGGGCTGTAATTCCCGATATCGCAAGTCTGGACGAG
  GTCCCTAAGATCTTCGAGGGTCTGGGAAGAGATGAAAAGCGGGCCGCAAACGCCCTTCAGCAGGAGAATA
  GCGAAAATGAGTCCCATTGCGGGGTCCTTGTCGAGCTGGAAGGAGACAACGCACGACTGGCAGTCCTCAA
  ACGGAGCATCGAGGTGACCCACTTCGCCTACCCTGCACTGAATCTGCCTCCCAAGGTAATGAGCACAGTG
  ATGTCTGAACTCATCGTTCGCAGAGCCAGACCATTGGAACGCGACGCTAACCTGCAGGAACAGACTGAGG
  AAGGGCTGCCAGCCGTCGGCGATGAACAATTGGCCAGGTGGCTTGAGACACGGGAGCCAGCCGATCTCGA
  GGAAAGACGAAAGCTCATGATGGCCGCCGTGCTTGTGACTGTCGAACTGGAATGTATGCAGAGATTCTTT
  GCAGATCCAGAAATGCAGCGAAAACTCGAAGAAACTCTCCATTATACATTTCGCCAGGGCTATGTGAGGC
  AAGCCTGTAAAATTTCCAATGTGGAGCTGTGCAATCTGGTGTCTTATCTCGGGATCTTGCATGAAAATAG
  GCTCGGCCAGAACGTACTGCACTCTACTTTGAAAGGGGAGGCTAGACGAGATTATGTGCGAGATTGCGTC
  TATCTCTTCCTGTGTTACACTTGGCAAACTGCAATGGGGGTATGGCAGCAATGCTTGGAGGAACGCAACC
  TGAAGGAACTGCAAAAGCTCCTTAAGCAGAACTTGAAGGATCTGTGGACCGCTTTCAATGAAAGAAGTGT
  GGCCGCTCATCTGGCGGATATTATCTTTCCCGAGAGACTGCTCAAAACCCTGCAACAGGGGCTGCCCGAT
  TTCACAAGCCAGAGTATGCTCCAGAATTTCCGGAACTTTATTCTGGAGCGATCTGGCATCCTGCCAGCTA
  CATGCTGCGCCCTTCCTAGCGACTTCGTCCCAATCAAGTACCGGGAGTGCCCACCACCACTGTGGGGCCA
  CTGTTATCTGCTGCAGTTGGCGAACTACCTCGCATACCATTCCGATATAATGGAAGATGTTAGCGGTGAT
  GGACTGCTTGAATGCCATTGTCGCTGCAATCTGTGTACACCACATCGGTCTCTGGTTTGCAACTCACAGC
  TGCTCTCCGAATCACAGATTATAGGCACATTCGAATTGCAAGGACCTAGCCCCGACGAAAAGTCTGCAGC
  ACCAGGTCTGAAGCTCACCCCAGGGCTTTGGACATCCGCCTATCTGCGGAAATTCGTACCAGAAGATTAC
  CACGCTCATGAGATTAGGTTCTATGAGGATCAGTCACGACCCCCCAATGCTGAACTGACAGCCTGCGTGA
  TCACACAAGGGCATATCCTGGGGCAACTCCAAGCAATAAACAAAGCCCGACAGGAGTTTCTGCTCAGGAA
  GGGAAGAGGAGTTTATCTTGACCCACAGAGTGGGGAGGAACTGAATCCTATCCCCCCACCTCCCCAACCT
  TATCAACAGCCAAGAGCCCTGGCAAGTCAGGACGGAACACAGAAAGAGGCTGCAGCAGCCGCCGCTGCGA
  CGCATGGTAGGGGAGGAATCCTTGGCCAGAGTGGAAGAGGGGGCTTTGGCAGAGGTGGGGGTGACGACGG
  CCGCCTTGGGCAGCCACGGAGATCCTTCAGGGGCCGGAGAGGTGTGAGACGGAACACGGTCACATTGGGC
  CGCATACCTCTTGCCGGTGCCCCTGAAATAGGAAATAGATCTCAGCATAGGTACAACCTGCGCTCAAGCG
  GCGCGGCCGGAACCGCGTGTTCACCTACCCAGCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100047 representing AP_000178
  Red=Cloning sites Green=Tags
  
  MESVEKEDSLTAPFEFATTASTDAANAPTTFPVEAPPLEEEEVIIEQDPGFVSEDDEDRSVPTEDKKQDQ
  DDAEANEEQVGRGDQRHGDYLDVGDDVLLKHLQRQCAIICDALQERSDVPLAIADVSLAYERHLFSPRVP
  PKRQENGTCEPNPRLNFYPVFAVPEVLATYHIFFQNCKIPLSCRANRSRADKQLALRQGAVIPDIASLDE
  VPKIFEGLGRDEKRAANALQQENSENESHCGVLVELEGDNARLAVLKRSIEVTHFAYPALNLPPKVMSTV
  MSELIVRRARPLERDANLQEQTEEGLPAVGDEQLARWLETREPADLEERRKLMMAAVLVTVELECMQRFF
  ADPEMQRKLEETLHYTFRQGYVRQACKISNVELCNLVSYLGILHENRLGQNVLHSTLKGEARRDYVRDCV
  YLFLCYTWQTAMGVWQQCLEERNLKELQKLLKQNLKDLWTAFNERSVAAHLADIIFPERLLKTLQQGLPD
  FTSQSMLQNFRNFILERSGILPATCCALPSDFVPIKYRECPPPLWGHCYLLQLANYLAYHSDIMEDVSGD
  GLLECHCRCNLCTPHRSLVCNSQLLSESQIIGTFELQGPSPDEKSAAPGLKLTPGLWTSAYLRKFVPEDY
  HAHEIRFYEDQSRPPNAELTACVITQGHILGQLQAINKARQEFLLRKGRGVYLDPQSGEELNPIPPPPQP
  YQQPRALASQDGTQKEAAAAAAATHGRGGILGQSGRGGFGRGGGDDGRLGQPRRSFRGRRGVRRNTVTLG
  RIPLAGAPEIGNRSQHRYNLRSSGAAGTACSPTQP
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000007
• ORF Size: 2415 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000007.1, AP_000178
• RefSeq ORF: 2415
• MW: 90.1 kDa