pVII (AC_000007) Virus Tagged ORF Clone

CAT#: VC100040

Myc-DDK-tagged ORF clone of viral ORF for pVII [Human adenovirus 2], codon optimized for human cell expression, AP_000171

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: pVII
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100040 represents NCBI reference of AP_000171 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCATACTGATAAGCCCTAGCAATAACACGGGGTGGGGGCTGCGCTTTCCGTCCAAGATGTTCGGCG
  GTGCGAAGAAGCGCTCTGACCAACATCCAGTGAGAGTTCGAGGACATTATCGCGCCCCATGGGGCGCCCA
  CAAGAGGGGCCGCACCGGCAGGACCACTGTCGACGATGCAATCGATGCTGTGGTGGAAGAGGCTCGGAAC
  TACACTCCAACCCCTCCACCCGTCTCCACCGTTGATGCCGCCATTCAGACGGTAGTTAGAGGAGCACGGA
  GATACGCCAAAATGAAAAGGCGGAGGCGGAGAGTGGCCAGAAGACATAGAAGGAGACCCGGAACGGCAGC
  CCAACGGGCTGCCGCTGCACTCTTGAACCGGGCACGGAGAACAGGAAGACGGGCTGCCATGAGAGCAGCC
  AGAAGACTGGCCGCTGGCATTGTTACAGTCCCCCCGCGGTCCCGGAGGAGAGCAGCTGCTGCAGCAGCCG
  CCGCTATCAGCGCCATGACCCAGGGAAGGAGAGGCAACGTATATTGGGTTCGGGATTCAGTCAGTGGTCT
  GCGCGTGCCTGTGCGCACCCGGCCTCCCAGAAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100040 representing AP_000171
  Red=Cloning sites Green=Tags
  
  MSILISPSNNTGWGLRFPSKMFGGAKKRSDQHPVRVRGHYRAPWGAHKRGRTGRTTVDDAIDAVVEEARN
  YTPTPPPVSTVDAAIQTVVRGARRYAKMKRRRRRVARRHRRRPGTAAQRAAAALLNRARRTGRRAAMRAA
  RRLAAGIVTVPPRSRRRAAAAAAAAISAMTQGRRGNVYWVRDSVSGLRVPVRTRPPRN
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000007
• ORF Size: 594 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000007.1, AP_000171
• RefSeq ORF: 594
• MW: 22.0 kDa