DBP (AC_000007) Virus Tagged ORF Clone

CAT#: VC100046

Myc-DDK-tagged ORF clone of viral ORF for DBP [Human adenovirus 2], codon optimized for human cell expression, AP_000177

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DBP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100046 represents NCBI reference of AP_000177 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATCCAGGGAGGAGGAGCAAAGGGAGACCACACCTGAGCGGGGCAGGGGTGCAGCCCGGAGACCAC
  CAACCATGGAGGATGTGTCATCACCGAGCCCCTCTCCCCCACCGCCAAGAGCGCCACCTAAAAAGCGATT
  GAGACGCCGGTTGGAGTCTGAAGACGAGGAGGATTCCTCCCAGGACGCGCTGGTGCCACGAACCCCTTCA
  CCTAGACCCTCAACATCTACAGCCGACCTGGCTATTGCAAGCAAGAAGAAGAAAAAGAGGCCCAGCCCCA
  AGCCCGAGCGGCCCCCCTCTCCTGAAGTGATCGTGGATAGCGAGGAGGAACGGGAGGACGTGGCTTTGCA
  GATGGTTGGATTCAGCAACCCTCCCGTGCTGATCAAACACGGAAAGGGCGGCAAACGGACCGTGCGGCGA
  TTGAACGAGGATGATCCTGTGGCACGGGGTATGCGGACTCAGGAAGAGAAAGAGGAGAGTTCAGAGGCAG
  AGTCTGAAAGCACGGTAATCAATCCTCTTAGCCTGCCTATCGTTTCAGCGTGGGAGAAGGGTATGGAGGC
  GGCTCGGGCTCTGATGGACAAATACCACGTTGATAACGACTTGAAGGCTAATTTCAAATTGCTGCCAGAT
  CAGGTGGAAGCCCTGGCCGCTGTGTGCAAAACTTGGCTGAATGAGGAGCATAGGGGGCTGCAGCTGACAT
  TTACCTCAAACAAAACTTTTGTCACTATGATGGGCAGGTTCCTGCAGGCCTACCTGCAGAGTTTCGCGGA
  GGTGACTTATAAGCACCACGAACCCACCGGCTGTGCACTGTGGCTGCACCGGTGCGCAGAGATAGAGGGA
  GAGCTTAAATGCTTGCACGGGAGTATAATGATCAATAAAGAGCATGTTATCGAGATGGATGTGACATCCG
  AGAACGGGCAGCGGGCTCTCAAGGAGCAGTCCTCAAAGGCAAAGATTGTCAAGAATAGGTGGGGTCGCAA
  CGTTGTCCAAATTTCAAACACCGATGCCCGCTGCTGCGTTCATGACGCGGCTTGCCCTGCTAACCAGTTC
  AGCGGGAAGTCCTGCGGCATGTTTTTCTCCGAGGGCGCCAAAGCTCAGGTGGCGTTTAAGCAGATCAAAG
  CCTTCATGCAGGCCCTGTACCCGAATGCCCAAACTGGTCACGGGCACCTGCTGATGCCACTGAGATGCGA
  GTGTAACAGTAAGCCTGGACACGCACCTTTTTTGGGTCGCCAGCTGCCCAAATTGACTCCCTTCGCACTC
  AGCAACGCTGAGGACCTGGATGCGGACCTGATCTCCGATAAATCTGTCTTGGCCTCAGTTCACCACCCCG
  CCCTTATTGTATTCCAATGTTGTAATCCTGTGTATCGCAACAGTCGCGCCCAAGGTGGAGGCCCTAATTG
  CGATTTCAAAATTTCAGCACCTGACCTTCTCAATGCACTTGTCATGGTGCGGAGTCTGTGGAGCGAGAAT
  TTCACAGAACTTCCCAGAATGGTGGTTCCAGAATTCAAGTGGAGTACTAAGCACCAGTATCGGAATGTAA
  GCCTGCCGGTGGCCCATAGCGATGCACGCCAGAACCCATTCGATTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100046 representing AP_000177
  Red=Cloning sites Green=Tags
  
  MASREEEQRETTPERGRGAARRPPTMEDVSSPSPSPPPPRAPPKKRLRRRLESEDEEDSSQDALVPRTPS
  PRPSTSTADLAIASKKKKKRPSPKPERPPSPEVIVDSEEEREDVALQMVGFSNPPVLIKHGKGGKRTVRR
  LNEDDPVARGMRTQEEKEESSEAESESTVINPLSLPIVSAWEKGMEAARALMDKYHVDNDLKANFKLLPD
  QVEALAAVCKTWLNEEHRGLQLTFTSNKTFVTMMGRFLQAYLQSFAEVTYKHHEPTGCALWLHRCAEIEG
  ELKCLHGSIMINKEHVIEMDVTSENGQRALKEQSSKAKIVKNRWGRNVVQISNTDARCCVHDAACPANQF
  SGKSCGMFFSEGAKAQVAFKQIKAFMQALYPNAQTGHGHLLMPLRCECNSKPGHAPFLGRQLPKLTPFAL
  SNAEDLDADLISDKSVLASVHHPALIVFQCCNPVYRNSRAQGGGPNCDFKISAPDLLNALVMVRSLWSEN
  FTELPRMVVPEFKWSTKHQYRNVSLPVAHSDARQNPFDF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000007
• ORF Size: 1587 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000007.1, AP_000177
• RefSeq ORF: 1587
• MW: 59.1 kDa