pVI (AC_000007) Virus Tagged ORF Clone

CAT#: VC100043

Myc-DDK-tagged ORF clone of viral ORF for pVI [Human adenovirus 2], codon optimized for human cell expression, AP_000174

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: pVI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100043 represents NCBI reference of AP_000174 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGACATAAATTTCGCCAGCCTTGCCCCTCGGCACGGATCACGGCCTTTCATGGGAAATTGGCAGG
  ACATTGGAACTAGTAACATGAGTGGCGGCGCGTTCTCATGGGGTAGCCTTTGGTCCGGCATCAAGAATTT
  CGGCTCTACCATCAAAAACTATGGCTCCAAGGCATGGAACAGTTCCACAGGGCAGATGCTGCGAGATAAA
  CTCAAAGAACAAAATTTCCAGCAGAAGGTGGTAGATGGCCTGGCCTCAGGGATTTCTGGAGTGGTTGACC
  TCGCTAATCAGGCCGTGCAGAATAAGATTAACAGCAAACTGGACCCTCGACCACCAGTTGAGGAACCACC
  CCCTGCTGTGGAAACGGTGAGCCCCGAGGGACGGGGGGAGAAGCGGCCCCGGCCCGATCGCGAGGAAACG
  CTGGTCACCCAAATTGACGAGCCCCCCTCTTATGAGGAGGCTCTGAAGCAGGGTCTCCCCACCACACGCC
  CTATAGCCCCTATGGCAACGGGGGTGTTGGGCCAGCACACCCCCGTCACGCTGGATCTGCCGCCACCCGC
  CGACACCCAGCAGAAGCCCGTGTTGCCAGGCCCTTCTGCTGTAGTTGTTACCAGACCTTCACGCGCGTCA
  CTGCGGAGGGCCGCGTCCGGACCCAGATCAATGCGGCCAGTTGCATCCGGGAACTGGCAGAGCACCCTCA
  ACAGCATAGTGGGCCTCGGAGTGCAGTCACTGAAACGCCGGCGCTGCTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100043 representing AP_000174
  Red=Cloning sites Green=Tags
  
  MEDINFASLAPRHGSRPFMGNWQDIGTSNMSGGAFSWGSLWSGIKNFGSTIKNYGSKAWNSSTGQMLRDK
  LKEQNFQQKVVDGLASGISGVVDLANQAVQNKINSKLDPRPPVEEPPPAVETVSPEGRGEKRPRPDREET
  LVTQIDEPPSYEEALKQGLPTTRPIAPMATGVLGQHTPVTLDLPPPADTQQKPVLPGPSAVVVTRPSRAS
  LRRAASGPRSMRPVASGNWQSTLNSIVGLGVQSLKRRRCF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000007
• ORF Size: 750 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000007.1, AP_000174
• RefSeq ORF: 750
• MW: 27.0 kDa