E1B 55K (AC_000007) Virus Tagged ORF Clone

CAT#: VC100056

Myc-DDK-tagged ORF clone of viral ORF for E1B 55K [Human adenovirus 2], codon optimized for human cell expression, AP_000163

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1B 55K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100056 represents NCBI reference of AP_000163 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAAGGCGCAACCCAAGCGAGCGGGGAGTGCCAGCCGGCTTTTCAGGCCACGCCTCAGTGGAGTCAG
  GTGGCGAGACACAAGAATCCCCGGCAACCGTGGTTTTCAGGCCACCTGGTAACAATACCGACGGTGGGGC
  GACTGCTGGAGGATCTCAGGCAGCTGCAGCGGCCGGCGCAGAACCCATGGAACCAGAGAGTCGACCTGGC
  CCAAGTGGAATGAACGTTGTGCAGGTAGCCGAGCTCTTTCCTGAGTTGCGGCGCATCTTGACTATCAATG
  AGGATGGTCAGGGACTGAAAGGCGTTAAACGGGAAAGAGGGGCTTCTGAGGCAACAGAGGAAGCTCGAAA
  CCTCACTTTTTCTCTCATGACACGGCATCGCCCAGAGTGTGTGACTTTTCAACAGATCAAGGATAACTGT
  GCCAACGAGTTGGACCTCCTTGCCCAGAAGTATAGCATTGAGCAGCTGACAACGTACTGGCTGCAACCAG
  GAGACGATTTTGAGGAGGCTATAAGAGTGTACGCTAAAGTCGCGCTGAGACCTGATTGCAAATACAAGAT
  TAGTAAGCTGGTGAACATCAGGAATTGTTGTTACATCAGTGGAAATGGGGCTGAGGTTGAAATAGACACC
  GAGGACCGAGTGGCCTTCCGGTGTAGTATGATAAATATGTGGCCGGGGGTGTTGGGCATGGATGGAGTGG
  TGATCATGAATGTTCGCTTTACAGGACCCAACTTCTCTGGAACAGTTTTCCTCGCCAACACTAACTTGAT
  CCTCCATGGGGTGTCTTTCTATGGCTTTAATAATACCTGCGTGGAGGCCTGGACGGATGTGAGAGTCAGG
  GGGTGTGCGTTTTACTGTTGTTGGAAGGGCGTCGTATGCCGGCCTAAGTCCCGGGCGTCCATCAAGAAAT
  GCCTGTTCGAGCGGTGCACATTGGGAATTCTGTCAGAAGGCAACAGTAGAGTGAGACATAACGTCGCATC
  TGACTGTGGTTGCTTTATGCTGGTCAAGTCCGTCGCAGTGATCAAACATAACATGGTGTGCGGCAATTGT
  GAGGACAGAGCATCCCAAATGCTGACTTGCTCTGATGGAAATTGTCACCTGCTGAAAACCATCCACGTGG
  CCTCCCATAGTAGGAAAGCATGGCCTGTGTTCGAACACAATATTTTGACACGCTGTTCTTTGCATTTGGG
  GAACCGCAGAGGTGTATTTCTGCCTTACCAATGCAACCTGTCACACACCAAAATCTTGCTGGAACCAGAG
  TCTATGAGCAAAGTGAATCTGAACGGCGTCTTTGACATGACAATGAAGATCTGGAAGGTACTCCGCTATG
  ACGAGACAAGGACGCGGTGTAGACCTTGCGAGTGCGGGGGCAAGCACATAAGAAATCAGCCTGTGATGCT
  CGACGTTACCGAGGAGTTGAGACCCGACCACCTGGTCCTTGCCTGTACACGCGCCGAGTTCGGCTCTTCC
  GACGAGGACACAGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100056 representing AP_000163
  Red=Cloning sites Green=Tags
  
  MERRNPSERGVPAGFSGHASVESGGETQESPATVVFRPPGNNTDGGATAGGSQAAAAAGAEPMEPESRPG
  PSGMNVVQVAELFPELRRILTINEDGQGLKGVKRERGASEATEEARNLTFSLMTRHRPECVTFQQIKDNC
  ANELDLLAQKYSIEQLTTYWLQPGDDFEEAIRVYAKVALRPDCKYKISKLVNIRNCCYISGNGAEVEIDT
  EDRVAFRCSMINMWPGVLGMDGVVIMNVRFTGPNFSGTVFLANTNLILHGVSFYGFNNTCVEAWTDVRVR
  GCAFYCCWKGVVCRPKSRASIKKCLFERCTLGILSEGNSRVRHNVASDCGCFMLVKSVAVIKHNMVCGNC
  EDRASQMLTCSDGNCHLLKTIHVASHSRKAWPVFEHNILTRCSLHLGNRRGVFLPYQCNLSHTKILLEPE
  SMSKVNLNGVFDMTMKIWKVLRYDETRTRCRPCECGGKHIRNQPVMLDVTEELRPDHLVLACTRAEFGSS
  DEDTD
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000007
• ORF Size: 1485 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000007.1, AP_000163
• RefSeq ORF: 1485
• MW: 54.9 kDa