III (AC_000007) Virus Tagged ORF Clone

CAT#: VC100039

Myc-DDK-tagged ORF clone of viral ORF for III [Human adenovirus 2], codon optimized for human cell expression, AP_000170

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: III
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100039 represents NCBI reference of AP_000170 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGAGAGCCGCAATGTACGAGGAGGGACCACCACCTAGCTACGAGTCAGTCGTAAGTGCCGCCCCTG
  TCGCTGCCGCTCTTGGGTCTCCCTTCGACGCCCCTCTTGACCCTCCTTTCGTTCCACCAAGATACCTGCG
  CCCAACCGGAGGAAGGAACAGTATCCGCTATTCCGAGCTTGCCCCTTTGTTCGATACTACCAGGGTTTAC
  CTTGTGGACAACAAGTCTACTGACGTTGCAAGCCTCAACTATCAGAACGATCACTCTAATTTTCTGACTA
  CAGTCATCCAGAATAATGACTACTCTCCAGGTGAGGCGTCTACACAGACAATTAATTTGGATGATCGATC
  TCATTGGGGCGGTGACCTTAAGACGATTCTGCATACCAATATGCCTAACGTAAATGAATTCATGTTCACC
  AACAAGTTTAAGGCCCGCGTAATGGTTAGTAGGAGTCTCACCAAAGACAAGCAAGTGGAGTTGAAGTATG
  AGTGGGTTGAATTCACGCTGCCAGAGGGTAACTATTCCGAGACCATGACCATCGACCTGATGAACAATGC
  AATCGTGGAACATTATCTGAAGGTGGGACGGCAGAATGGAGTGCTTGAGTCTGATATCGGGGTTAAGTTT
  GATACTAGGAACTTCCGCCTGGGGTTCGACCCCGTGACTGGCCTGGTAATGCCAGGCGTGTATACAAACG
  AGGCCTTCCATCCCGATATCATCCTTCTGCCCGGATGTGGCGTGGACTTCACCCACTCCAGGCTGAGCAA
  CCTGCTGGGCATCAGGAAAAGGCAGCCCTTCCAGGAGGGATTCCGAATCACTTATGATGATCTTGAAGGC
  GGAAACATCCCAGCTCTGCTCGACGTCGATGCTTACCAGGCTAGCCTGAAGGACGACACAGAACAAGGCG
  GCGATGGAGCCGGCGGGGGAAACAACAGCGGCAGCGGCGCAGAAGAAAACAGTAATGCCGCAGCTGCCGC
  CATGCAGCCAGTAGAAGATATGAACGATCATGCCATTAGGGGAGACACTTTCGCTACTCGGGCTGAGGAA
  AAAAGGGCCGAAGCCGAAGCAGCCGCCGAGGCCGCCGCTCCAGCTGCGCAGCCCGAAGTCGAAAAGCCCC
  AGAAAAAGCCTGTGATCAAGCCACTGACAGAGGATTCAAAAAAACGGAGTTACAACCTGATCTCAAATGA
  CTCAACTTTCACCCAATATCGAAGCTGGTACCTGGCCTACAACTACGGGGACCCACAAACAGGCATCAGG
  TCCTGGACACTTCTGTGTACCCCAGACGTGACATGCGGTTCAGAACAGGTATACTGGTCTCTCCCCGACA
  TGATGCAAGACCCTGTCACATTCAGATCAACCTCACAAATATCAAATTTTCCAGTGGTAGGCGCTGAACT
  CCTGCCAGTGCACTCAAAATCTTTTTACAATGATCAAGCAGTGTATTCCCAGCTCATTCGCCAATTTACT
  TCACTTACCCATGTGTTCAACAGGTTCCCCGAAAACCAAATACTGGCACGCCCGCCTGCCCCCACAATCA
  CCACAGTGTCCGAGAACGTGCCGGCCCTCACCGATCACGGAACCCTGCCACTGCGCAACTCCATTGGTGG
  TGTTCAAAGAGTCACAATAACTGACGCGCGGAGGCGGACCTGCCCCTACGTGTATAAGGCACTCGGGATC
  GTCTCTCCACGGGTGCTCTCTAGCCGGACATTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100039 representing AP_000170
  Red=Cloning sites Green=Tags
  
  MQRAAMYEEGPPPSYESVVSAAPVAAALGSPFDAPLDPPFVPPRYLRPTGGRNSIRYSELAPLFDTTRVY
  LVDNKSTDVASLNYQNDHSNFLTTVIQNNDYSPGEASTQTINLDDRSHWGGDLKTILHTNMPNVNEFMFT
  NKFKARVMVSRSLTKDKQVELKYEWVEFTLPEGNYSETMTIDLMNNAIVEHYLKVGRQNGVLESDIGVKF
  DTRNFRLGFDPVTGLVMPGVYTNEAFHPDIILLPGCGVDFTHSRLSNLLGIRKRQPFQEGFRITYDDLEG
  GNIPALLDVDAYQASLKDDTEQGGDGAGGGNNSGSGAEENSNAAAAAMQPVEDMNDHAIRGDTFATRAEE
  KRAEAEAAAEAAAPAAQPEVEKPQKKPVIKPLTEDSKKRSYNLISNDSTFTQYRSWYLAYNYGDPQTGIR
  SWTLLCTPDVTCGSEQVYWSLPDMMQDPVTFRSTSQISNFPVVGAELLPVHSKSFYNDQAVYSQLIRQFT
  SLTHVFNRFPENQILARPPAPTITTVSENVPALTDHGTLPLRNSIGGVQRVTITDARRRTCPYVYKALGI
  VSPRVLSSRTF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000007
• ORF Size: 1713 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000007.1, AP_000170
• RefSeq ORF: 1713
• MW: 63.3 kDa