E1B 19K (AC_000007) Virus Tagged ORF Clone

CAT#: VC100055

Myc-DDK-tagged ORF clone of viral ORF for E1B 19K [Human adenovirus 2], codon optimized for human cell expression, AP_000162

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1B 19K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100055 represents NCBI reference of AP_000162 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCCTGGGAATGTCTGGAGGACTTCAGCGCTGTCCGGAATCTGCTTGAACAGAGCAGCAATAGTA
  CTTCATGGTTCTGGAGGTTTCTGTGGGGGTCCAGTCAAGCGAAGCTTGTCTGCCGAATCAAAGAAGACTA
  TAAATGGGAATTTGAAGAGCTGCTCAAGTCATGCGGGGAGCTGTTCGATAGTCTGAATCTCGGACACCAG
  GCTCTCTTCCAAGAGAAGGTGATTAAAACTCTGGATTTTTCAACACCAGGACGCGCAGCGGCCGCCGTGG
  CTTTTCTGAGCTTCATAAAAGATAAGTGGAGCGAAGAAACTCACTTGTCAGGTGGCTATCTGCTGGATTT
  CCTGGCGATGCATCTGTGGCGGGCAGTGGTACGCCACAAGAACCGGCTCCTCCTGCTGTCTAGTGTGCGG
  CCCGCCATCATCCCTACCGAGGAACAGCAGCAGGAAGAGGCCAGGAGAAGACGACGACAAGAACAGAGTC
  CCTGGAACCCCCGGGCAGGTCTTGACCCTAGAGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100055 representing AP_000162
  Red=Cloning sites Green=Tags
  
  MEAWECLEDFSAVRNLLEQSSNSTSWFWRFLWGSSQAKLVCRIKEDYKWEFEELLKSCGELFDSLNLGHQ
  ALFQEKVIKTLDFSTPGRAAAAVAFLSFIKDKWSEETHLSGGYLLDFLAMHLWRAVVRHKNRLLLLSSVR
  PAIIPTEEQQQEEARRRRRQEQSPWNPRAGLDPRE
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000007
• ORF Size: 525 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000007.1, AP_000162
• RefSeq ORF: 525
• MW: 20.5 kDa