E3 RID beta (AC_000007) Virus Tagged ORF Clone

CAT#: VC100065

Myc-DDK-tagged ORF clone of viral ORF for E3 RID beta [Human adenovirus 2], codon optimized for human cell expression, AP_000187

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E3 RID beta
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100065 represents NCBI reference of AP_000187 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCGGAGCGTGATATTTGTCCTTCTGATTTTTTGCGCACTGCCCGTGCTGTGCTCTCAGACAAGCG
  CCCCCCCAAAGCGGCACATCAGCTGTAGGTTCACTCAGATATGGAATATCCCTTCATGCTACAATAAGCA
  GTCAGATCTCAGTGAGGCTTGGCTGTACGCTATTATTTCCGTTATGGTGTTCTGCTCCACCATATTTGCC
  CTGGCAATTTATCCTTATCTTGATATCGGCTGGAATGCTATCGACGCAATGAACCACCCTACCTTTCCGG
  TGCCTGCTGTAATCCCCCTCCAACAGGTCATAGCACCGATTAACCAACCCAGACCTCCTTCTCCAACCCC
  CACCGAGATTTCTTACTTCAATCTTACGGGCGGGGACGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100065 representing AP_000187
  Red=Cloning sites Green=Tags
  
  MKRSVIFVLLIFCALPVLCSQTSAPPKRHISCRFTQIWNIPSCYNKQSDLSEAWLYAIISVMVFCSTIFA
  LAIYPYLDIGWNAIDAMNHPTFPVPAVIPLQQVIAPINQPRPPSPTPTEISYFNLTGGDD
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000007
• ORF Size: 390 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000007.1, AP_000187
• RefSeq ORF: 390
• MW: 14.5 kDa