E4 ORF4 (AC_000007) Virus Tagged ORF Clone

CAT#: VC100069

Myc-DDK-tagged ORF clone of viral ORF for E4 ORF4 [Human adenovirus 2], codon optimized for human cell expression, AP_000193

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E4 ORF4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100069 represents NCBI reference of AP_000193 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCTCCCTGCCCTGCCAGCTCCCCCAGTTTGTGACTCACAGAACGAGTGCGTAGGCTGGCTGGGGG
  TGGCGTACTCAGCGGTGGTGGATGTGATCAGGGCTGCTGCACACGAGGGTGTATACATCGAACCTGAGGC
  CCGGGGCCGGCTTGATGCCCTGAGGGAGTGGATCTATTACAACTACTATACCGAGAGGGCCAAACGACGC
  GATCGACGGAGGCGCAGCGTGTGTCACGCCCGAACCTGGTTTTGTTTCCGGAAGTATGACTACGTGCGGA
  GGTCTATCTGGCACGATACCACGACTAACACTATCAGCGTTGTCTCCGCTCACTCTGTGCAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100069 representing AP_000193
  Red=Cloning sites Green=Tags
  
  MVLPALPAPPVCDSQNECVGWLGVAYSAVVDVIRAAAHEGVYIEPEARGRLDALREWIYYNYYTERAKRR
  DRRRRSVCHARTWFCFRKYDYVRRSIWHDTTTNTISVVSAHSVQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000007
• ORF Size: 342 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000007.1, AP_000193
• RefSeq ORF: 342
• MW: 13.3 kDa