V (AC_000007) Virus Tagged ORF Clone

CAT#: VC100041

Myc-DDK-tagged ORF clone of viral ORF for V [Human adenovirus 2], codon optimized for human cell expression, AP_000172

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: V
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100041 represents NCBI reference of AP_000172 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTAAGAGGAAAATCAAGGAAGAGATGCTGCAGGTCATAGCACCCGAGATCTACGGGCCACCTAAGA
  AGGAAGAACAAGACTACAAACCCAGGAAACTCAAGCGGGTCAAAAAGAAGAAGAAAGATGACGATGATGA
  TGAGCTGGACGACGAGGTGGAGCTGCTGCATGCTACGGCTCCTAGACGGCGGGTCCAGTGGAAAGGGAGA
  AGAGTTCGCCGCGTGCTGAGGCCGGGCACAACTGTGGTGTTCACACCCGGCGAGAGATCTACTAGGACCT
  ATAAAAGAGTCTACGATGAGGTGTACGGTGATGAAGATCTCCTTGAACAGGCAAACGAACGCCTGGGAGA
  GTTCGCGTACGGCAAGCGCCATAAAGACATGCTGGCTCTGCCCCTGGACGAAGGAAATCCTACACCCTCT
  TTGAAGCCTGTGACACTGCAGCAGGTGTTGCCCACACTGGCCCCCTCTGAAGAGAAACGCGGACTCAAAA
  GAGAGTCCGGGGATCTCGCTCCAACTGTCCAGCTGATGGTACCTAAAAGGCAAAGACTGGAAGATGTTCT
  GGAGAAAATGACCGTAGAGCCTGGCCTTGAACCTGAAGTGCGAGTGCGCCCTATCAAGCAGGTGGCACCA
  GGCCTGGGAGTTCAGACAGTTGATGTGCAGATCCCCACTACATCAAGCACTAGCATTGCAACAGCGACGG
  AAGGAATGGAAACACAGACCTCTCCCGTCGCTAGTGCCGTGGCCGATGCTGCCGTGCAAGCCGCCGCCGC
  TGCAGCTTCTAAGACATCTACCGAGGTGCAGACAGATCCCTGGATGTTCCGGGTATCAGCCCCAAGGAGG
  CCTCGCAGAAGCAGAAAGTATGGAACCGCATCAGCTCTGTTGCCCGAATACGCTCTCCATCCCTCAATAG
  CTCCCACTCCCGGCTATCGGGGTTATACCTACAGGCCACGCCGGCGCGCTACCACAAGACGCCGCACAAC
  CACCGGAACACGGCGCAGGAGACGAAGGCGGCAGCCTGTGCTTGCACCCATATCCGTGCGGAGAGTCGCA
  AGAGAGGGTGGCCGCACCCTGGTCCTTCCTACTGCACGGTATCACCCCAGCATTGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100041 representing AP_000172
  Red=Cloning sites Green=Tags
  
  MSKRKIKEEMLQVIAPEIYGPPKKEEQDYKPRKLKRVKKKKKDDDDDELDDEVELLHATAPRRRVQWKGR
  RVRRVLRPGTTVVFTPGERSTRTYKRVYDEVYGDEDLLEQANERLGEFAYGKRHKDMLALPLDEGNPTPS
  LKPVTLQQVLPTLAPSEEKRGLKRESGDLAPTVQLMVPKRQRLEDVLEKMTVEPGLEPEVRVRPIKQVAP
  GLGVQTVDVQIPTTSSTSIATATEGMETQTSPVASAVADAAVQAAAAAASKTSTEVQTDPWMFRVSAPRR
  PRRSRKYGTASALLPEYALHPSIAPTPGYRGYTYRPRRRATTRRRTTTGTRRRRRRRQPVLAPISVRRVA
  REGGRTLVLPTARYHPSIV
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000007
• ORF Size: 1107 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000007.1, AP_000172
• RefSeq ORF: 1107
• MW: 41.7 kDa